CoronaVIR: Collection and compilation of Computational Resources on Novel Coronavirus (SARS-CoV-2 or COVID-19)

Welcome to Page of Predicted Diagnostic Primers for nCOV-2019 (CoVID-19)

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This page provides the information of Predicted Diagnostic Primers which can be used for the diagnosis of CoVID-19. These primers are predicted by Primer3_Core tool with default parameters. These primers are designated from the complete genome of 53 different nucleotide sequences samples taken from NCBI Virus Resource. These primers can be considered as "Universal" primer pair sets as these are common among all the samples.

Note: These primers need experimental Validation.

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Table representing the predicted universal primers by Primer3_core of nCoV-2019 strain diagnosis

ID	Fwd	Rev	Oligo	Fwd_length	Rev_length	Oligo_length	Fwd_Tm	Rev_Tm	Oligo_Tm	Fwd_GC	Rev_GC	Oligo_GC	Target_size
CornaVIR_1	CTCTTCTCGTTCCTCATCACG	CCAGACATTTTGCTCTCAAGC	TTGCTGCTGCTTGACAGATT	21	21	20	59.997	60.008	59.746	52.381	47.619	45	162
CornaVIR_2	AGTCAAGCCTCTTCTCGTTCC	CCAGACATTTTGCTCTCAAGC	TTGCTGCTGCTTGACAGATT	21	21	20	60.009	60.008	59.746	52.381	47.619	45	170
CornaVIR_3	GTCAAGCCTCTTCTCGTTCCT	CCAGACATTTTGCTCTCAAGC	TTGCTGCTGCTTGACAGATT	21	21	20	60.009	60.008	59.746	52.381	47.619	45	169
CornaVIR_4	AGCCTCTTCTCGTTCCTCATC	CCAGACATTTTGCTCTCAAGC	TTGCTGCTGCTTGACAGATT	21	21	20	59.972	60.008	59.746	52.381	47.619	45	165
CornaVIR_5	GGGGGACAACCAATCACTAAT	TAACCTTTCCACATACCGCAG	CAGTTACACCGGAAGCCAAT	21	21	20	59.933	60.004	59.993	47.619	47.619	50	278