CoronaVIR: Collection and compilation of Computational Resources on Novel Coronavirus (SARS-CoV-2 or COVID-19)

Welcome to Page of Diagnostic Primers

--------------------------------------------------------------------------------------------------------------------------------------------------------

This page provides the information of Predicted Diagnostic Primers which can be used for the diagnosis of CoVID-19. These primers are predicted by Primer3_Core tool with default parameters. These primers are designated for ORF1ab-gene of the strain based in template sequences of the genes taken from NCBI Virus Resource.

Note: These primers need experimental Validation.

--------------------------------------------------------------------------------------------------------------------------------------------------------

Table representing the predicted primers by Primer3_core for ORF1ab-gene of nCoV-2019 strain diagnosis

Template Source ID	Template Source Country	Gene	Fwd_Primer seq	Rev_Primer seq	oligo seq	Fwd_Primer Start Position	Fwd_Length	Rev_Primer Start Position	Rev_Length	Oligo Start pos	Oligo_Length	Target_Product size	Fwd_Primer Tm	Rev_Primer Tm	Oligo Tm	Fwd (GC%)	Rev (GC%)	Oligo (GC%)
MT163738	Iran	ORF1ab	ACCGCAAGGTTCTTCTTCGTA	CGGGTAACACCACTGCTATGT	GGAGCTGGTGGCCATAGTTA	117	21	276	21	152	20	160	61.147	59.928	60.096	47.619	52.381	55
MT163738	Iran	ORF1ab	ACCGCAAGGTTCTTCTTCGTA	ACGGGTAACACCACTGCTATG	GGAGCTGGTGGCCATAGTTA	117	21	277	21	152	20	161	61.147	59.928	60.096	47.619	52.381	55
MT163738	Iran	ORF1ab	ACCGCAAGGTTCTTCTTCGTA	CACGGGTAACACCACTGCTAT	GGAGCTGGTGGCCATAGTTA	117	21	278	21	152	20	162	61.147	59.928	60.096	47.619	52.381	55
MT163738	Iran	ORF1ab	CCGCAAGGTTCTTCTTCGTA	CGGGTAACACCACTGCTATGT	GGAGCTGGTGGCCATAGTTA	118	20	276	21	152	20	159	60.378	59.928	60.096	47.619	52.381	55
MT163738	Iran	ORF1ab	CCGCAAGGTTCTTCTTCGTA	ACGGGTAACACCACTGCTATG	GGAGCTGGTGGCCATAGTTA	118	20	277	21	152	20	160	60.378	59.928	60.096	50	52.381	55
MT072667	Belgium	ORF1ab	TTGCGTAGTGATGTGCTATTACC	GAGAGCCTTTGCGAGATGAC	TGGAGCAATGGATACAACTAGC	517	23	654	20	594	22	138	59.223	60.104	59.252	43.478	55	45.455
MT072667	Belgium	ORF1ab	GTTGCGTAGTGATGTGCTATTACC	GAGAGCCTTTGCGAGATGAC	TGGAGCAATGGATACAACTAGC	516	24	654	20	594	22	139	59.984	60.104	59.252	45.833	55	45.455
MT072667	Belgium	ORF1ab	TTGCGTAGTGATGTGCTATTACCT	GAGAGCCTTTGCGAGATGAC	TGGAGCAATGGATACAACTAGC	517	24	654	20	594	22	138	60.102	60.104	59.252	41.667	55	45.455
MT072667	Belgium	ORF1ab	TGCGTAGTGATGTGCTATTACC	GAGAGCCTTTGCGAGATGAC	TGGAGCAATGGATACAACTAGC	518	22	654	20	594	22	137	57.889	60.104	59.252	45.455	55	45.455
MT072667	Belgium	ORF1ab	TGCGTAGTGATGTGCTATTACCT	GAGAGCCTTTGCGAGATGAC	TGGAGCAATGGATACAACTAGC	518	23	654	20	594	22	137	58.87	60.104	59.252	43.478	55	45.455