CoronaVIR: Collection and compilation of Computational Resources on Novel Coronavirus (SARS-CoV-2 or COVID-19)

Welcome to Page of Diagnostic Primers

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This page provides the information of Predicted Diagnostic Primers which can be used for the diagnosis of CoVID-19. These primers are predicted by Primer3_Core tool with default parameters. These primers are designated for M-gene of the strain based in template sequences of the genes taken from NCBI Virus Resource.

Note: These primers need experimental Validation.

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Table representing the predicted primers by Primer3_core for M-gene of nCoV-2019 strain diagnosis

Template Source ID	Template Source country	Gene	Fwd_Primer seq	Rev_Primer seq	oligo seq	Fwd_Primer Start Position	Fwd_Length	Rev_Primer Start Position	Rev_Length	Oligo Start pos	Oligo_Length	Target_Product size	Fwd_Primer Tm	Rev_Primer Tm	Oligo Tm	Fwd (GC%)	Rev (GC%)	Oligo (GC%)
MT008023, MT008022	Itlay	M	TTGTGCTTGCTGCTGTTTAC	TACGAAGATGTCCACGAAGGA	TTCAGACTGTTTGCGCGTAC	12	20	269	21	116	20	258	57.723	60.642	60.058	45	47.619	50
MT008023, MT008022	Itlay	M	TTGTGCTTGCTGCTGTTTAC	ACGAAGATGTCCACGAAGGAT	TTCAGACTGTTTGCGCGTAC	12	20	268	21	116	20	257	57.723	60.898	60.058	45	47.619	50
MT008023, MT008022	Itlay	M	TTTTGTGCTTGCTGCTGTTTA	CAATACGAAGATGTCCACGA	TTCAGACTGTTTGCGCGTAC	10	20	272	21	116	20	263	59.679	57.118	60.058	38.095	45	50
MT008023, MT008022	Itlay	M	TTGTGCTTGCTGCTGTTTAC	ATACGAAGATGTCCACGAAGG	TTCAGACTGTTTGCGCGTAC	12	20	270	21	116	20	259	57.723	59.066	60.058	45	47.619	50
MT008023, MT008022	Itlay	M	TTGTGCTTGCTGCTGTTTAC	CGAAGATGTCCACGAAGGAT	TTCAGACTGTTTGCGCGTAC	12	20	267	21	116	20	256	57.723	60.073	60.058	45	50	50