CoronaVIR: Collection and compilation of Computational Resources on Novel Coronavirus (SARS-CoV-2 or COVID-19)

Welcome to Page of Diagnostic Primers

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This page provides the information of Predicted Diagnostic Primers which can be used for the diagnosis of CoVID-19. These primers are predicted by Primer3_Core tool with default parameters. These primers are designated for complete genome of CoVID-19 strain (53 different genome sequences) and various genes, i.e. N, ORF1ab, E, M, and ORF1ab-RdRP of the strain based on their sequences obtained from NCBI Virus Resource.

Note: These primers need experimental Validation.

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Predicted primers by Primer3_core for Diagnosis of nCoV-2019 strain

Table representing the predicted universal primers (based on 53 complete genome sequences) by Primer3_core of nCoV-2019 strain diagnosis

ID	Fwd	Rev	Oligo	Fwd_length	Rev_length	Oligo_length	Fwd_Tm	Rev_Tm	Oligo_Tm	Fwd_GC	Rev_GC	Oligo_GC	Target_size
CornaVIR_1	CTCTTCTCGTTCCTCATCACG	CCAGACATTTTGCTCTCAAGC	TTGCTGCTGCTTGACAGATT	21	21	20	59.997	60.008	59.746	52.381	47.619	45	162
CornaVIR_2	AGTCAAGCCTCTTCTCGTTCC	CCAGACATTTTGCTCTCAAGC	TTGCTGCTGCTTGACAGATT	21	21	20	60.009	60.008	59.746	52.381	47.619	45	170
CornaVIR_3	GTCAAGCCTCTTCTCGTTCCT	CCAGACATTTTGCTCTCAAGC	TTGCTGCTGCTTGACAGATT	21	21	20	60.009	60.008	59.746	52.381	47.619	45	169
CornaVIR_4	AGCCTCTTCTCGTTCCTCATC	CCAGACATTTTGCTCTCAAGC	TTGCTGCTGCTTGACAGATT	21	21	20	59.972	60.008	59.746	52.381	47.619	45	165
CornaVIR_5	GGGGGACAACCAATCACTAAT	TAACCTTTCCACATACCGCAG	CAGTTACACCGGAAGCCAAT	21	21	20	59.933	60.004	59.993	47.619	47.619	50	278