Summary of Modifications in Cell Lines



This page is designed to facilitate users to browse assays where particular modification in cell line has been done to study the various aspects of Herceptin resistance.

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Serial No. Alterations in Cell lines Number Of Assays Number Of Cell lines
1 BT474 modified to resistant clone BT-HRc3, Infected with lentiviral GDF15 shRNA (shGDF)11
2 BT474 modified to resistant clone BT474/TR201
3 BT474 modified to resistant clone BT474/TR, Treadted with 2-DEOXY-D-GLUCOSE (Glycolysis inhibitor) 41
4 BT474 modified to resistant clone BT474/TR, Treadted withOXAMATE (Glycolysis inhibitor) 41
5 Make cell line resistant to trastuzumab121
6 NSC23766 (Inhibitor of RAC1)11
7 SKBR3 modified to resistant SKBR3/mAbHER211
8 SKBR3 modified to resistant SKBR3/mAbHER311
9 SKBR3 modified to resistant SKBR3/mAbHER411
10 SKBR3 modified to resistant SKBR3/mAbHER511
11 SKBR3 modified to resistant SKBR3/mAbHER611
12 SKBR3 modified to resistant SKBR3/mAbHER711
13 Trasfected with GRB7 siRNA153
14 Trasfected with GRB7 siRNA with Trasfected with HER2 siRNA33
15 Trasfected with GSDM1 siRNA123
16 Trasfected with HER2 siRNA423
17 Trasfected with PERLD1 siRNA123
18 Trasfected with PPP1R1B siRNA153
19 Trasfected with PPP1R1B siRNA with Trasfected with HER2 siRNA 33
20 Trasfected with PSMD3 siRNA153
21 Trasfected with PSMD3 siRNA with Trasfected with HER2 siRNA33
22 Trasfected with SCRAMBLE siRNA243
23 Trasfected with STARD3 siRNA63
24 Trasfected with STARD3 siRNA with Trasfected with HER2 siRNA33
25 Trasfected with THRA siRNA153
26 Trasfected with THRA siRNA with Trasfected with HER2 siRNA 33
27 Trasfected with ZPBP2 siRNA153
28 Trasfected with ZPBP2 siRNA with Trasfected with HER2 siRNA 33
29 Trastuzumab resistant BT474 (BT474R)81
30 Trastuzumab resistant SKBR3 (SKBR-3R)81
31 Treated with Laminin rich extracellular matrix culture (IrECM)161
3217-AAG (Down regulate ERBB2)102
33AU565 Culture in 2D MONOLAYER 61
34AU565 Culture in 3D IrECM61
35Anti-Estrogen inhibitor (Fulvestrant) , Treated MCF7Ca with Letrozole for 56 week 11
36Anti-Estrogen inhibitor (Fulvestrant)r , Treated MCF7Ca with Letrozole for 56 week 11
37Anti-Estrogen inhibitor (Tamoxifen ) , Treated MCF7Ca with Letrozole for 56 week 21
38Aromatase inhibitor (Anastrozole ), Treated MCF7Ca with letrozole for 56 week 21
39Aromatase inhibitor (Exemestane ), Treated MCF7Ca with letrozole for 56 week 21
40BT474 Transfected with p110a subunit of PI3K (BT474H1047R)81
41BT474 modifie to resitanr clone BT474-TR, Inhibition of Akt and S6K phosphorylation21
42BT474 modified to clone BT474 SC91
43BT474 modified to clone BT474-c331
44BT474 modified to resistant BT474 (HR2), Trasfected by RshRNA to knockdown IGF-IR82
45BT474 modified to resistant clone BT-HRc3, Infected with lentiviral Control sh RNA (shC)11
46BT474 modified to resistant clone BT/HerR121
47BT474 modified to resistant clone BT474 /TR31
48BT474 modified to resistant clone BT474 HR551
49BT474 modified to resistant clone BT474 HR651
50BT474 modified to resistant clone BT474- HR541
51BT474 modified to resistant clone BT474- HR641
52BT474 modified to resistant clone BT474-HR2091
53BT474 modified to resistant clone BT474-HRc191
54BT474 modified to resistant clone BTres1201
55BT474 modified to resistant clone BTres261
56BT474 varient51
57BT549 Culture in 2D MONOLAYER 61
58BT549 Culture in 3D IrECM61
59Cell line modified to MCF-7/HER2111
60Cell treated with Glycolysis inhibitor204
61Cell treated with Glycolysis inhibitor, BT474 modified to resistant clone BT474/TR41
62Cell treated with lactate dehydrogenase inhibitor 204
63Cell treated with lactate dehydrogenase inhibitor , BT474 modified to resistant clone BT474/TR41
64Cyclin D kinase 4/6 inhibitor (PD 0332991)183
65Ectopic expression of CYCLIN E21
66Empty vector203
67Empty vector tranfected in MCF-7/HER-2/EV21
68Empty vector tranfected in SKBR3-EV151
69Estrogen receptor inhibition, Cell line modified to MCF-7/HER251
70Exogenous stimulation by BTC11
71Exogenous stimulation by EGF11
72Exogenous stimulation by HRG 11
73Expression of cleaved caspase 311
74Expression of scrambled SIRNA 11
75Expression of scrambled SIRNA, Make cell line resistant to trastuzumab11
76HCC1569 Culture in 2D MONOLAYER 61
77HCC1569 Culture in 3D IrECM61
78HCC1954 treated with LY294002 ( PI3K inhibitor)11
79HER2 silencing in BT47411
80HER2 silencing in JIMT411
81HER2 silencing in KPL411
82HER2 silencing in MCF711
83HER2 silencing in SKBR311
84High express cyclinE, Make cell line resistant to trastuzumab21
85High express p2731
86High express p27, Make cell line resistant to trastuzumab21
87IGF-IR TRANSFECTED, SKBR3 modified to clone SKBR3/IGF-IR131
88Infected with lentiviral Control sh RNA (shC)53
89Infected with lentiviral GDF15 shRNA (shGDF)53
90Inhibition of ADAM17 121
91Inhibition of Akt and S6K phosphorylation108
92Inhibition of Topo IIα41
93Inhibition of all panHER receptors 61
94Inhibitor of IGF-IR tyrosine kinase121
95Knockdown ATG1231
96Knockdown of CYCLIN E by specific Si RNA21
97Knockdown of CYCLIN E by specific Si RNA, Make cell line resistant to trastuzumab11
98MCF-7 exposed to rHuEPO, MCF7 modified to clone MCF7-HER-1871
99MCF10A modified to MCF10A. HER2 81
100MCF7 modified to clone MCF7-HER-18341
101MCF7 modified to clone MCF7/HER231
102MCF7 transfected by erbB-2 (MCF7/erbB2)41
103MDA-MB-453 exposed to rHuEPO21
104MDA-MB-453 modified to resistant cell line (MDA-MB-453R)81
105MOCK TRANSFECTED, SKBR3 modified to clone SKBR3/neo131
106MOCK TRANSFECTED, SKBR3 modified to clone SKBR3/neo 21
107MODIFICATION OF CELL LINE 11
108Make cell line resistant to trastuzumab161
109Met receptor knockdown 42
110NF-kB downregulation21
111No expression of cleaved caspase 331
112None88245
113Over express TIMP-1 11
114Over express TIMP-1, SKBR3 modified to clone SKBR3 C2 CLONE11
115Over express TIMP-1, SKBR3 modified to clone SKBR3 C3 CLONE11
116Over express TIMP-1, SKBR3 modified to clone SKBR3 C4 CLONE11
117Over express TIMP-1, SKBR3 modified to clone SKBR3 C5 CLONE11
118Over express TIMP-1, SKBR3 modified to clone SKBR3 CI CLONE11
119PCDGF/GP88 Cdna stably transfected in SKBR3 (SKBR3-GP88)31
120PI3K INHIBITOR321
121PI3K INHIBITOR, Trasfected by MYR-AKT1, SKBR3 modified to clone SKBR3/AA2811
122PI3K INHIBITOR,Trasfected by MYR-AKT1, SKBR3 modified to clone SKBR3/AA2811
123PIK3CA mt124
124PIK3CA wt124
125Parental SKBR3101
126Parental SKBR3, transfected with 100 ng of pCMV-FLAG-p27kip131
127Parental SKBR3, transfected with 25 ng of pCMV-FLAG-p27kip131
128Parental SKBR3, transfected with vector control31
129Pretreated with GDF15 for 48 HRS72
130SK-HRc3 (SKBR3 Resistant cell line), Infected with lentiviral Control sh RNA (shC)11
131SK-HRc3 (SKBR3 Resistant cell line), Infected with lentiviral GDF15 shRNA (shGDF)11
132SKBR3 Culture in 2D MONOLAYER 61
133SKBR3 Culture in 3D IrECM61
134SKBR3 Resistant cell line (SKBR3- HR), Tranfected with HSP27 SiRNA41
135SKBR3 Resistant cell line (SKBR3- HR), Tranfected with control NS SiRNA41
136SKBR3 exposed to rHuEPO41
137SKBR3 modified to resistant SKBR3 (POOL2), Trasfected by B3shRNA to knockdown erbB322
138SKBR3 modified to resistant SKBR3 (POOL2), Trasfected by B3shRNA to knockdown erbB422
139SKBR3 modified to resistant SKBR3 (POOL2), Trasfected by B3shRNA to knockdown erbB522
140SKBR3 modified to resistant SKBR3 (POOL2), Trasfected by B3shRNA to knockdown erbB622
141SKBR3 modified to resistant cell line (SKBR3R)81
142SKBR3 modified to resistant clone SKBR3-HRp2191
143SKBR3 modified to resistant clone SKBR3-HRp3191
144SKBR3 modified to resistant clone SKBR3-R, Transfected with dominant negative SPRY2Y55F21
145SKBR3 modified to resistant clone SKBR3-R, Transfected with dominant negative SPRY2Y55F 11
146SKBR3 modified to resistant clone SKBR3-pool291
147SKBR3 modified to resistant clone SKBR3/R51
148SKBR3 modified to resistant clone SKBR3/R, Trasfected with EPOR shRNA 11
149SKBR3 modified to resistant clone SKBR3/R, Trasfected with srambled shRNA11
150Stable GDF15 overexpresed in BT474 (GDFc2)Clone61
151Stable GDF15 overexpresed in BT474 (GDFc3)Clone61
152Stable GDF15 overexpresed in SKBR3 (GDFc3) Clone11
153Stable GDF15 overexpresed in SKBR3 (GDFc4) Clone11
154Stable GDF15 overexpresed in SKBR3 (GDFc5) Clone11
155T47D trasfected by HER231
156T47D trasfected by P95her231
157TRANFECTED BY MYR-AKT1 , BT474 modified to clone BT474/AA911
158TRANFECTED BY MYR-AKT1, BT474 modified to clone BT474/AA911
159TRANFECTED BY MYR-AKT1, SKBR3 modified to clone SKBR3/AA2861
160TRANFECTED BY dominant negative akt1, BT474 modified to clone BT474/DN521
161TRANFECTED BY dominant negative akt1, SKBR3 modified to clone SKBR3/DN951
162TRANFECTED BY pUSEamp with , BT474 modified to clone BT474/V21
163TRANFECTED BY pUSEamp with , SKBR3 modified to clone SKBR3/V3351
164TRANSDUCED PTEN ANTISENCE 11
165TRANSFECTED BY pBabe161
166TRANSFECTED BY pBabe-FoxM181
167Tamoxifen Resistant cell line 61
168Transdused by CA-EphA242
169Transdused by CA-EphA2, MCF10A modified to MCF10A. HER2 21
170Transdused by KD-EPHA2 to knockdown 21
171Transdused by KD-EPHA2 to knockdown, MCF10A modified to MCF10A. HER2 21
172Transdused by antisence PTEN72
173Transdused by mismatched oligonucleotide52
174Transfected and overexpress with Alk5TD21
175Transfected with HER2 and overexpressed 126
176Transfected with HSF1 SiRNA122
177Transfected with HSF1 SiRNA222
178Transfected with P3xflag PTEN mut, BT474 modified to resistant clone R-BT47461
179Transfected with P3xflagvector alone, BT474 modified to resistant clone R-BT47461
180Transfected with PCDNA3.0/TDARPP.A vector41
181Transfected with PCDNA3.0/TDARPP.A with DARPP-32 D vector 61
182Transfected with SKBR3 PLENTI7.321
183Transfected with SKBR3-IGF-IR to overexpress IGF-IR201
184Transfected with SKBR3-IGF-IR to overexpress IGF-IR with Trasfected with nonspecific siRNA11
185Transfected with SKBR3-IGF-IR to overexpress IGF-IR with Trasfected with specific Tiam 1 siRNA11
186Transfected with SPRY242
187Transfected with SPRY2, SKBR3 modified to resistant clone SKBR3-R21
188Transfected with anti-miR-20011
189Transfected with dominant negative SPRY2Y55F42
190Transfected with empty PCDNA3.0/NEO1 vector21
191Transfected with empty PCDNA3.0/NEO2 vector21
192Transfected with empty vector42
193Transfected with empty vector, SKBR3 modified to resistant clone SKBR3-R21
194Transfected with miR-21 MIMICS283
195Transfected with miR-21 mimics181
196Transfected with miR-21 mimics with p3XFLAG121
197Transfected with miR-21 mimics with p3XFLAG-PTEN-MUT61
198Transfected with miR-21ASO, BT474 modified to resistant clone R-BT474263
199Transfected with miR-21ASO, MDA-MB-453 modified to resistant clone R-MDA-MB-45321
200Transfected with miR-21ASO, SKBR3modified to resistant clone R-SKBR321
201Transfected with non specific Lin4 MIMICS283
202Transfected with non specific Lin4ASO, BT474 modified to resistant clone R-BT474263
203Transfected with non specific Lin4ASO, MDA-MB-453 modified to resistant clone R-MDA-MB-45321
204Transfected with non specific Lin4ASO, SKBR3modified to resistant clone R-SKBR321
205Transfected with non specific mock703
206Transfected with non specific mock, BT474 modified to resistant clone R-BT474323
207Transfected with non specific mock, MDA-MB-453 modified to resistant clone R-MDA-MB-45321
208Transfected with non specific mock, SKBR3 modified to resistant clone R-SKBR321
209Transfected with nonspecific siCtrl 21
210Transfected with siSLC to knock down SLC25A4321
211Transfeted with IGF1R si-RNA82
212Transfeted with empty vector and overexpresed 41
213Transfeted with scrambled siRNA112
214Transfeted with t-DARP siRNA 31
215Transfeted with t-DARPP-1 and overexpresed81
216Transfeted with t-DARPPsiRNA 31
217Trasfected BY specific SiRNA- FoxM1 11
218Trasfected by EMPTY VECTOR Pcdna3, MCF7 modified to HER-2 overexpress clone MCF7-HER221
219Trasfected by FOXO1A83
220Trasfected by FOXO1A, BT474 modified to clone BT474/AA921
221Trasfected by FOXO1A, MCF7 modified to HER-2 overexpress clone MCF7-HER221
222Trasfected by FOXO1A, SKBR3 modified to clone SKBR3/AA2821
223Trasfected by P-1257 peptide63
224Trasfected by P-SCR peptide 63
225Trasfected by empty vecto Pcdna3, SKBR3 modified to clone SKBR3/AA2811
226Trasfected by empty vector Pcdna352
227Trasfected by empty vector Pcdna3, BT474 modified to clone BT474/AA921
228Trasfected by empty vector Pcdna3, SKBR3 modified to clone SKBR3/AA2811
229Trasfected by non specific SiRNA162
230Trasfected by non specific SiRNA 112
231Trasfected by non specific SiRNA, MDA-MB-453 modified to resistant cell line (MDA-MB-453R)21
232Trasfected by non specific SiRNA, SKBR3 modified to resistant cell line (SKBR3R)21
233Trasfected by pBabe303
234Trasfected by pBabe-FoxM1153
235Trasfected by specific SiRNA- FoxM1132
236Trasfected by specific SiRNA- FoxM1 92
237Trasfected by specific SiRNA- FoxM1, MDA-MB-453 modified to resistant cell line (MDA-MB-453R)11
238Trasfected by specific SiRNA- FoxM1, SKBR3 modified to resistant cell line (SKBR3R)21
239Trasfected by specific SiRNA- FoxM1, SKBR3 modified to resistant cell line (SKBR3R) 11
240Trasfected by specific- FoxM1 CONTROL11
241Trasfected by specific- FoxM1, MDA-MB-453 modified to resistant cell line (MDA-MB-453R) 11
242Trasfected with Control shRNA31
243Trasfected with Ctrl siRNA121
244Trasfected with Ctrl siRNA, BT474 modified to resistant clone BTres161
245Trasfected with EPOR shRNA 21
246Trasfected with GSDM1 siRNA with Trasfected with HER2 siRNA 33
247Trasfected with HER2 siRNA483
248Trasfected with PERLD1 siRNA with Trasfected with HER2 siRNA33
249Trasfected with SIRNA against IQGAP(SIIQ12)101
250Trasfected with SIRNA against IQGAP(SIIQ12) and resistant SIRNA21
251Trasfected with SIRNA against IQGAP(SIIQ12) and vector21
252Trasfected with SIRNA against IQGAP(SIIQ12) and vector 11
253Trasfected with SIRNA against IQGAP(SIIQ12), SKBR3 modified to resistant clone SKBR3-TR21
254Trasfected with SIRNA against IQGAP(SIIQ14), SKBR3 modified to resistant clone SKBR3-TR21
255Trasfected with SIRNA against RENILLA LUSIFERASE(SIREN)101
256Trasfected with SIRNA against RENILLA LUSIFERASE(SIREN) and vector61
257Trasfected with SIRNA against RENILLA LUSIFERASE(SIREN) and vector, SKBR3 modified to resistant clone SKBR3-TR11
258Trasfected with SIRNA against RENILLA LUSIFERASE(SIREN) and vector,SKBR3 modified to resistant clone SKBR3-TR11
259Trasfected with SIRNAs against IQGAP AND P27(SIIQ12 AND SIP27)41
260Trasfected with SKBR3-PTEN-sh13 to knock down PTEN201
261Trasfected with SKBR3-PTEN-sh13 to knock down PTEN with Trasfected with nonspecific siRNA11
262Trasfected with SKBR3-PTEN-sh13 to knock down PTEN with Trasfected with specific Tiam 1 siRNA11
263Trasfected with SKBR3-PTEN-sh19 to knock down PTEN181
264Trasfected with SKBR3-Pmko.1 emptyvector 21
265Trasfected with STARD3 siRNA153
266Trasfected with antisense PTEN, BT474 modified to clone BT474.m1321
267Trasfected with antisense PTEN, BT474 modified to clone BT474.m1 31
268Trasfected with non specific oligonucleoted, BT474 modified to clone BT474.m1 141
269Trasfected with non specific shRNA21
270Trasfected with specific CTRL SIRNA 11
271Trasfected with specific CTRL SIRNA, Transfected with Alk5TD11
272Trasfected with specific ERBB3 SIRNA 21
273Trasfected with specific ERBB3 SIRNA , Transfected with Alk5TD11
274Trasfected with specific MUC1 shRNA41
275Trasfected with specific MUC1 siRNA, BT474 modified to resistant clone BTres161
276Trasfected with specific TACE SIRNA 11
277Trasfected with specific TACE SIRNA, Transfected with Alk5TD11
278Trasfected with srambled shRNA 11
279Trastuzuamb Resistant SKBR3 (pool1)191
280Trastuzuamb Resistant SKBR3 (pool1), a-IR3 blocks the receptor-binding domain of IGF-IR61
281Trastuzuamb Resistant SKBR3 (pool1), transfected with 100 ng of pCMV-FLAG-p27kip131
282Trastuzuamb Resistant SKBR3 (pool1), transfected with 25 ng of pCMV-FLAG-p27kip131
283Trastuzuamb Resistant SKBR3 (pool1), transfected with vector control31
284Trastuzuamb Resistant SKBR3 (pool2)281
285Trastuzuamb Resistant SKBR3 (pool2), Inhibitor of IGF-IR tyrosine kinase61
286Trastuzuamb Resistant SKBR3 (pool2), a-IR3 blocks the receptor-binding domain of IGF-IR61
287Trastuzuamb Resistant SKBR3 (pool2), transfected with 100 ng of pCMV-FLAG-p27kip131
288Trastuzuamb Resistant SKBR3 (pool2), transfected with 25 ng of pCMV-FLAG-p27kip131
289Trastuzuamb Resistant SKBR3 (pool2), transfected with vector control31
290Trastuzumab resistant refractory pool 2 BT474 (BT-HRp3)31
291Trastuzumab resistant refractory pool 2 SKBR3 (SK-HRp2)31
292Treadted with 2-DEOXY-D-GLUCOSE (Glycolysis inhibitor) 163
293Treadted withOXAMATE (Glycolysis inhibitor) 163
294Treated MCF7Ca with Letrozole for 56 week 111
295Treated MCF7Ca with letrozole for 56 week 121
296Treated with 17-AAG ( degrade HER2 protein )21
297Treated with 17β-ESTRADIOL(E2) 21
298Treated with ABT-37 (Bcl-2 inhibitor)62
299Treated with ABT-37 (Bcl-2 inhibitor), BT474 modified to clone BT474-c321
300Treated with ABT-37 (Bcl-2 inhibitor), BT474 modified to resistant clone BT474-HRc121
301Treated with CBZ ( HDAC6 inhibitor )31
302Treated with CBZ ( HDAC6 inhibitor ) with Treated with 17-AAG ( degrade HER2 protein )11
303Treated with Curcumin to inhibit signal transduction pathway(PI3K/AKT, MAPK, NF-KB)151
304Treated with EVEROLIMUS (Mtor inhibitor) 51
305Treated with EVEROLIMUS (Mtor inhibitor), BT474 modified to clone BT474 SC71
306Treated with IGF-IR Inhibitor (NVP-AEW541) 203
307Treated with LY294002 (PI3K inhibitor ), BT474 modified to resistant clone BT474-HRc121
308Treated with LY294002(Inhibitor of PI3K)21
309Treated with NVP-AEW541( IGF1R INHIBITOR )82
310Treated with NVP-AEW541( IGF1R INHIBITOR ), BT474 modified to resistant clone BT474 /TR21
311Treated with NaB (HISTONE DEACETYLASE inhibitor )42
312Treated with PBMC mononuclear cells ratio to target cells (80:1)93
313Treated with PPAR GAAMMA inhibitor TGZ (TROGLITAZONE)41
314Treated with PPAR GAAMMA inhibitor TGZ (TROGLITAZONE), MCF7 modified to clone MCF7/HER221
315Treated with SF1126 (PI3K INHIBITOR), BT474 modified to resistant clone BT474-HRp241
316Treated with SF1126 (PI3K INHIBITOR), BT474 modified to resistant clone BT474-HRp341
317Treated with SF1126 (PI3K INHIBITOR), SKBR3 modified to resistant clone SKBR3-HRp2101
318Treated with SF1126 (PI3K INHIBITOR), SKBR3 modified to resistant clone SKBR3-HRp3101
319Treated with erythropoitin( EPO)63
320Treated withPD98059(Inhibitor of MEK)21
321Treated withWEDELOLACTONE (IKK inhibitor ), BT474 modified to resistant clone BT474-HRc121
322Treatment with heregulin HRGβ1 (10 ng/ml)21
323UACC839 treated with LY294002 ( PI3K inhibitor)11
324WILD TYPE21
325Wild type 31
326control, BT474 modified to clone BT474/AA921
327control, BT474 modified to clone BT474/DN521
328control, MCF7 modified to HER-2 overexpress clone MCF7-HER221
329erbB-2 tranfected MCF7/erbB231
330knockdown siRNA FOXO1A, BT474 modified to clone BT474/DN521
331knockdown siRNA FOXO1A, MCF7 modified to HER-2 overexpress clone MCF7-HER221
332knockdown siRNA NEGATIVE CONTROL42
333knockdown siRNA NEGATIVE CONTROL, BT474 modified to clone BT474/DN521
334knockdown siRNA NEGATIVE CONTROL, MCF7 modified to HER-2 overexpress clone MCF7-HER211
335low express cyclinE11
336β1-Integrin vector transfected in MCF-7/HER-2/β121
337β1-Integrin vector transfected in SKBR3/β1-C1121