CoReMicrob: Computational Resources for Micorbiome

Major Tools for genome assembly from metagenomics data

This page provides informating reagrding the tools used to assemble genomes from the complex metagenomic samples, called as metagenome assembled genomes (MAG's).

Metagenome assembly tools

Tool Name Description PubMed ID
IBDA-UD A de novo assembler for single-cell and metagenomic sequencing data with highly uneven depth. 22495754
MEGAHIT A fast and scalable metagenome assembler driven by advanced methodologies and community practices. 27012178
metaSPAdes SPAdes is a versatile toolkit designed for assembly and analysis of sequencing data. SPAdes is primarily developed for Illumina sequencing data, but can be used for IonTorrent as well. 28298430
MetaVelvet A de novo metagenome assembly from short sequence reads. 22821567
MetaVelvet-SL An extension of the Velvet assembler to a de novo metagenomic assembler utilizing supervised learning. 25431440
MetaVelvet-DL A MetaVelvet deep learning extension for de novo metagenome assembly. 34078257
metaplasmidSPAdes Plasmid detection and assembly in genomic and metagenomic data sets. 31048319
metaviralSPAdes A assembly of viruses from metagenomic data. 32413137
Vclust Ultrafast and accurate sequence alignment and clustering of viral genomes. bioRxiv
OPERA-MS Hybrid metagenomic assembly enables high-resolution analysis of resistance determinants and mobile elements in human microbiomes. 31359005
Canu A scalable and accurate long-read assembly via adaptive k-mer weighting and repeat separation. 28298431
metaFlye A scalable long-read metagenome assembly using repeat graphs. 33020656
NECAT Efficient assembly of nanopore reads via highly accurate and intact error correction. 33397900
MECAT Fast mapping, error correction, and de novo assembly for single-molecule sequencing reads. 28945707
wtdbg2 A de novo sequence assembler for long noisy reads produced by PacBio or Oxford Nanopore Technologies (ONT). 31819265
Shasta A long read assembler. 32686750
metaMDBG High-quality metagenome assembly from long accurate reads. 38168989
SMAGLinker Integrate single amplified genome (SAG) and metagenome to reconstruct qualified microbial genomes. 34641955
ccSAG Obtaining high-quality draft genomes from uncultured microbes by cleaning and co-assembly of single-cell amplified genomes. 29391438

Major Tools for Assembly Quality Check

Tool Name Description PubMed ID
MetaQuast For evaluation of metagenome assemblies. 26614127
DeepMAsED For evaluating the quality of metagenomic assemblies. 32096824
REAPR A universal tool for genome assembly evaluation. 23710727
CheckM Assessing the quality of microbial genomes recovered from isolates, single cells, and metagenomes. 25977477
BUSCO Assessing Genome Assembly and Annotation Completeness. 31020564
CheckV Assesses the quality and completeness of metagenome-assembled viral genomes. 33349699
AMBER Assessment of Metagenome BinnERs. 29893851

Major Tools for OTU Clustering

Tool Name Description PubMed ID
Vsearch The tools for OTU clustering, diversity analyses, and taxonomic assignment. 27781170
UPARSE UPARSE is a method for generating clusters (OTUs) from next-generation sequencing reads of marker genes such as 16S rRNA. 23955772
OptiClust Improved method for assigning amplicon-based sequence data to operational taxonomic units. 28289728