Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004251                                                              21     229
NP_783864                                                              17    4312
NP_055928                                                              17    4312
NP_006257                                                              17    4312
NP_001035827                                                           17    4312
NP_001258705                                                           17    4312
NP_001258704                                                           17    4312
NP_001258703                                                           17    4312
XP_005256618                                                           17    4312
XP_005256617                                                           17    4312
XP_005262701                                                           17    4312
NP_036466                                                              16    5631
XP_006714538                                                           16    5631
NP_001138                                                              16    7355
NP_001112359                                                           16    7355
NP_001122312                                                           16    7355
NP_001122311                                                           16    7355
NP_000711                                                              16    7355
XP_005265505                                                           16    7355
XP_006713388                                                           16    7355
NP_001271230                                                           15    9605
NP_001271231                                                           15    9605
NP_061155                                                              15    9605
NP_525023                                                              15    9605
XP_006721855                                                           15    9605
XP_006721854                                                           15    9605
XP_006721853                                                           15    9605
XP_005249968                                                           15    9605
XP_005249966                                                           15    9605
XP_006715864                                                           15    9605
XP_005249965                                                           15    9605
XP_006715810                                                           15    9605
XP_006715809                                                           15    9605

>NP_004251
          Length = 1208

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   YVQLNMKQK 9
           YV+LNMKQK
Sbjct: 353 YVRLNMKQK 361



>NP_783864
          Length = 1044

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   YVQLNMKQK 9
           YV  N KQK
Sbjct: 480 YVDFNSKQK 488



>NP_055928
          Length = 2961

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   YVQLNMKQ 8
           YVQ+N+K+
Sbjct: 356 YVQINIKR 363



>NP_006257
          Length = 914

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YVQLNM 6
           YVQLNM
Sbjct: 227 YVQLNM 232



>NP_001035827
          Length = 859

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YVQLNM 6
           YVQLNM
Sbjct: 172 YVQLNM 177



>NP_001258705
          Length = 902

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YVQLNM 6
           YVQLNM
Sbjct: 215 YVQLNM 220



>NP_001258704
          Length = 913

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YVQLNM 6
           YVQLNM
Sbjct: 226 YVQLNM 231



>NP_001258703
          Length = 885

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YVQLNM 6
           YVQLNM
Sbjct: 198 YVQLNM 203



>XP_005256618
          Length = 2962

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   YVQLNMKQ 8
           YVQ+N+K+
Sbjct: 356 YVQINIKR 363



>XP_005256617
          Length = 2966

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   YVQLNMKQ 8
           YVQ+N+K+
Sbjct: 356 YVQINIKR 363



>XP_005262701
          Length = 1035

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   YVQLNMKQK 9
           YV  N KQK
Sbjct: 480 YVDFNSKQK 488



>NP_036466
          Length = 2058

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YVQLNMKQK 9
           +VQLN+ QK
Sbjct: 224 FVQLNICQK 232



>XP_006714538
          Length = 2035

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YVQLNMKQK 9
           +VQLN+ QK
Sbjct: 224 FVQLNICQK 232



>NP_001138
          Length = 496

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YVQLNMKQK 9
           Y+Q NMK++
Sbjct: 100 YIQDNMKKE 108



>NP_001112359
          Length = 495

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YVQLNMKQK 9
           Y+Q NMK++
Sbjct: 100 YIQDNMKKE 108



>NP_001122312
          Length = 2161

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           +LNMK+K
Sbjct: 850 ELNMKEK 856



>NP_001122311
          Length = 2137

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           +LNMK+K
Sbjct: 850 ELNMKEK 856



>NP_000711
          Length = 2181

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           +LNMK+K
Sbjct: 870 ELNMKEK 876



>XP_005265505
          Length = 2146

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           +LNMK+K
Sbjct: 850 ELNMKEK 856



>XP_006713388
          Length = 2194

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           +LNMK+K
Sbjct: 870 ELNMKEK 876



>NP_001271230
          Length = 1087

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           Q+N+KQK
Sbjct: 676 QVNIKQK 682



>NP_001271231
          Length = 1086

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           Q+N+KQK
Sbjct: 675 QVNIKQK 681



>NP_061155
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           Q+N+KQK
Sbjct: 713 QVNIKQK 719



>NP_525023
          Length = 1617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4 LNMKQK 9
         +NMKQK
Sbjct: 1 MNMKQK 6



>XP_006721855
          Length = 1551

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4 LNMKQK 9
         +NMKQK
Sbjct: 1 MNMKQK 6



>XP_006721854
          Length = 1577

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4 LNMKQK 9
         +NMKQK
Sbjct: 1 MNMKQK 6



>XP_006721853
          Length = 1617

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4 LNMKQK 9
         +NMKQK
Sbjct: 1 MNMKQK 6



>XP_005249968
          Length = 673

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           +LNM+QK
Sbjct: 663 ELNMEQK 669



>XP_005249966
          Length = 948

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           +LNM+QK
Sbjct: 938 ELNMEQK 944



>XP_006715864
          Length = 957

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           +LNM+QK
Sbjct: 947 ELNMEQK 953



>XP_005249965
          Length = 958

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           +LNM+QK
Sbjct: 948 ELNMEQK 954



>XP_006715810
          Length = 1105

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           Q+N+KQK
Sbjct: 676 QVNIKQK 682



>XP_006715809
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   QLNMKQK 9
           Q+N+KQK
Sbjct: 713 QVNIKQK 719



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.126    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 425,923
Number of extensions: 700
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)