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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     390
NP_001075109                                                           20     390
NP_000724                                                              16    7355
NP_001008777                                                           16    7355
NP_076924                                                              16    7355
NP_997300                                                              15    9605
NP_001269886                                                           15    9605
NP_001186711                                                           15    9605
XP_005260625                                                           15    9605
XP_005250316                                                           15    9605
NP_036304                                                              15    9605
NP_001156971                                                           15    9605
NP_277041                                                              15    9605

>NP_008835
          Length = 4128

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    YVAVSRGSK 9
            YVAV+RGSK
Sbjct: 4077 YVAVARGSK 4085



>NP_001075109
          Length = 4097

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    YVAVSRGSK 9
            YVAV+RGSK
Sbjct: 4046 YVAVARGSK 4054



>NP_000724
          Length = 207

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   YVAVSRGSK 9
           YV   RGSK
Sbjct: 96  YVCYPRGSK 104



>NP_001008777
          Length = 452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  YVAVSRGSK 9
          Y++ S GSK
Sbjct: 81 YISTSSGSK 89



>NP_076924
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   AVSRGSK 9
           AVSRG+K
Sbjct: 95  AVSRGAK 101



>NP_997300
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   YVAVSRGS 8
           Y+A++RG+
Sbjct: 156 YIAMNRGA 163



>NP_001269886
          Length = 284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   YVAVSRGS 8
           Y+A++RG+
Sbjct: 120 YIAMNRGA 127



>NP_001186711
          Length = 406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  VAVSRGS 8
          +AVSRGS
Sbjct: 21 LAVSRGS 27



>XP_005260625
          Length = 121

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   VSRGSK 9
           VSRGSK
Sbjct: 111 VSRGSK 116



>XP_005250316
          Length = 561

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YVAVSRGSK 9
           YV + RG+K
Sbjct: 215 YVVLCRGAK 223



>NP_036304
          Length = 618

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YVAVSRGSK 9
           YV + RG+K
Sbjct: 215 YVVLCRGAK 223



>NP_001156971
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YVAVSRGSK 9
           YV + RG+K
Sbjct: 165 YVVLCRGAK 173



>NP_277041
          Length = 580

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YVAVSRGSK 9
           YV + RG+K
Sbjct: 177 YVVLCRGAK 185



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.123    0.319 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 495,205
Number of extensions: 695
Number of successful extensions: 13
Number of sequences better than 10000.0: 13
Number of HSP's gapped: 13
Number of HSP's successfully gapped: 13
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)