Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064592                                                              17    2528
NP_065816                                                              17    2528
XP_005252384                                                           17    2528
XP_006710573                                                           17    2528
XP_006710572                                                           17    2528
XP_006710571                                                           17    2528
XP_006710570                                                           17    2528
XP_006710569                                                           17    2528
XP_006710568                                                           17    2528
XP_006710567                                                           17    2528
XP_006710566                                                           17    2528
XP_005245859                                                           17    2528
NP_001165599                                                           16    5631
NP_757366                                                              16    5631
NP_009111                                                              16    5631
NP_776173                                                              16    5631
XP_005276340                                                           16    5631
XP_005254400                                                           16    5631
XP_005254399                                                           16    5631
XP_006719419                                                           16    5631
XP_006719416                                                           16    5631
XP_006719415                                                           16    5631
XP_006719414                                                           16    5631
XP_006719412                                                           16    5631
XP_006719411                                                           16    5631
XP_005277735                                                           16    5631
XP_005249981                                                           16    5631
NP_001810                                                              16    7355
NP_001121113                                                           15    9605
NP_001017402                                                           15    9605
NP_000219                                                              15    9605
NP_065896                                                              15    9605
XP_006719582                                                           15    9605
XP_005253643                                                           15    9605
XP_005253642                                                           15    9605
XP_006719581                                                           15    9605
XP_006719580                                                           15    9605
XP_005253640                                                           15    9605
XP_006719579                                                           15    9605
XP_005253639                                                           15    9605
XP_005273181                                                           15    9605
>NP_064592
          Length = 1561

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    YSRKSAQFQ 9
            Y RKS +FQ
Sbjct: 1343 YKRKSVKFQ 1351
>NP_065816
          Length = 5183

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    YSRKSAQF 8
            Y RK+AQF
Sbjct: 3461 YGRKAAQF 3468
>XP_005252384
          Length = 841

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YSRKSAQFQ 9
           Y RKS +FQ
Sbjct: 623 YKRKSVKFQ 631
>XP_006710573
          Length = 5177

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    YSRKSAQF 8
            Y RK+AQF
Sbjct: 3455 YGRKAAQF 3462
>XP_006710572
          Length = 5188

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    YSRKSAQF 8
            Y RK+AQF
Sbjct: 3466 YGRKAAQF 3473
>XP_006710571
          Length = 5194

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    YSRKSAQF 8
            Y RK+AQF
Sbjct: 3472 YGRKAAQF 3479
>XP_006710570
          Length = 5195

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    YSRKSAQF 8
            Y RK+AQF
Sbjct: 3473 YGRKAAQF 3480
>XP_006710569
          Length = 5212

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    YSRKSAQF 8
            Y RK+AQF
Sbjct: 3490 YGRKAAQF 3497
>XP_006710568
          Length = 5221

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    YSRKSAQF 8
            Y RK+AQF
Sbjct: 3499 YGRKAAQF 3506
>XP_006710567
          Length = 5222

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    YSRKSAQF 8
            Y RK+AQF
Sbjct: 3500 YGRKAAQF 3507
>XP_006710566
          Length = 5222

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    YSRKSAQF 8
            Y RK+AQF
Sbjct: 3500 YGRKAAQF 3507
>XP_005245859
          Length = 5223

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1    YSRKSAQF 8
            Y RK+AQF
Sbjct: 3501 YGRKAAQF 3508
>NP_001165599
          Length = 1331

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SRKSAQFQ 9
          SRKSA+F+
Sbjct: 10 SRKSAKFR 17
>NP_757366
          Length = 1332

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SRKSAQFQ 9
          SRKSA+F+
Sbjct: 10 SRKSAKFR 17
>NP_009111
          Length = 583

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YSRKSAQFQ 9
           YS+KSA ++
Sbjct: 506 YSQKSAMYE 514
>NP_776173
          Length = 758

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSRKSAQ 7
           YSRKSA+
Sbjct: 677 YSRKSAK 683
>XP_005276340
          Length = 158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  YSRKSAQ 7
          YSRKSA+
Sbjct: 87 YSRKSAK 93
>XP_005254400
          Length = 1378

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SRKSAQFQ 9
          SRKSA+F+
Sbjct: 57 SRKSAKFR 64
>XP_005254399
          Length = 1379

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SRKSAQFQ 9
          SRKSA+F+
Sbjct: 57 SRKSAKFR 64
>XP_006719419
          Length = 443

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSRKSAQ 7
           YSRKSA+
Sbjct: 362 YSRKSAK 368
>XP_006719416
          Length = 615

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSRKSAQ 7
           YSRKSA+
Sbjct: 534 YSRKSAK 540
>XP_006719415
          Length = 615

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSRKSAQ 7
           YSRKSA+
Sbjct: 534 YSRKSAK 540
>XP_006719414
          Length = 708

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSRKSAQ 7
           YSRKSA+
Sbjct: 627 YSRKSAK 633
>XP_006719412
          Length = 724

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSRKSAQ 7
           YSRKSA+
Sbjct: 677 YSRKSAK 683
>XP_006719411
          Length = 738

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSRKSAQ 7
           YSRKSA+
Sbjct: 657 YSRKSAK 663
>XP_005277735
          Length = 552

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YSRKSAQFQ 9
           YS+KSA ++
Sbjct: 475 YSQKSAMYE 483
>XP_005249981
          Length = 158

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1  YSRKSAQ 7
          YSRKSA+
Sbjct: 87 YSRKSAK 93
>NP_001810
          Length = 677

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YSRKSAQF 8
           Y +KSA+F
Sbjct: 613 YRKKSAEF 620
>NP_001121113
          Length = 1172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   YSRKSAQFQ 9
           Y RK  QF+
Sbjct: 696 YQRKREQFE 704
>NP_001017402
          Length = 1172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   YSRKSAQFQ 9
           Y RK  QF+
Sbjct: 696 YQRKREQFE 704
>NP_000219
          Length = 1172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   YSRKSAQFQ 9
           Y RK  QF+
Sbjct: 696 YQRKREQFE 704
>NP_065896
          Length = 1349

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SRKSAQF 8
           SRK AQF
Sbjct: 254 SRKMAQF 260
>XP_006719582
          Length = 1295

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SRKSAQF 8
           SRK AQF
Sbjct: 254 SRKMAQF 260
>XP_005253643
          Length = 1331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SRKSAQF 8
           SRK AQF
Sbjct: 188 SRKMAQF 194
>XP_005253642
          Length = 1343

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SRKSAQF 8
           SRK AQF
Sbjct: 254 SRKMAQF 260
>XP_006719581
          Length = 1380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SRKSAQF 8
           SRK AQF
Sbjct: 254 SRKMAQF 260
>XP_006719580
          Length = 1381

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SRKSAQF 8
           SRK AQF
Sbjct: 254 SRKMAQF 260
>XP_005253640
          Length = 1397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SRKSAQF 8
           SRK AQF
Sbjct: 254 SRKMAQF 260
>XP_006719579
          Length = 1397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SRKSAQF 8
           SRK AQF
Sbjct: 254 SRKMAQF 260
>XP_005253639
          Length = 1397

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   SRKSAQF 8
           SRK AQF
Sbjct: 254 SRKMAQF 260
>XP_005273181
          Length = 1172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   YSRKSAQFQ 9
           Y RK  QF+
Sbjct: 696 YQRKREQFE 704
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.117    0.306 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 338,737
Number of extensions: 401
Number of successful extensions: 41
Number of sequences better than 10000.0: 41
Number of HSP's gapped: 41
Number of HSP's successfully gapped: 41
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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