Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              20     509
NP_001075109                                                           20     509
NP_005671                                                              18    1135
NP_116753                                                              18    1935
NP_116754                                                              18    1935
NP_001265548                                                           18    1935
NP_116755                                                              18    1935
NP_001186480                                                           17    3301
NP_001186552                                                           17    3301
NP_001186481                                                           17    3301
NP_071377                                                              17    3301
XP_005265433                                                           17    3301
XP_005265432                                                           17    3301
XP_005265431                                                           17    3301
NP_002259                                                              16    5631
NP_001243089                                                           16    5631
NP_079273                                                              16    5631
NP_006257                                                              16    5631
NP_001035827                                                           16    5631
NP_001258705                                                           16    5631
NP_001258704                                                           16    5631
NP_001258703                                                           16    5631
NP_001001922                                                           16    5631
XP_006721017                                                           16    5631
XP_006721016                                                           16    5631
NP_055752                                                              16    7355
NP_001230124                                                           16    7355
XP_006717783                                                           16    7355
XP_006717782                                                           16    7355
NP_579875                                                              15    9605
NP_064525                                                              15    9605
NP_002368                                                              15    9605
NP_758847                                                              15    9605
NP_056060                                                              15    9605
NP_001138238                                                           15    9605
NP_000112                                                              15    9605
NP_001191008                                                           15    9605
NP_002449                                                              15    9605
NP_690001                                                              15    9605
NP_001191010                                                           15    9605
NP_001191009                                                           15    9605
NP_001190994                                                           15    9605
NP_001190996                                                           15    9605
NP_003306                                                              15    9605
NP_001190999                                                           15    9605
NP_001190998                                                           15    9605
NP_858056                                                              15    9605
NP_001190995                                                           15    9605
NP_001190997                                                           15    9605
NP_002282                                                              15    9605
XP_006722117                                                           15    9605
XP_006722116                                                           15    9605
XP_006722115                                                           15    9605
XP_006722114                                                           15    9605
XP_006722113                                                           15    9605
XP_005269089                                                           15    9605
XP_006719577                                                           15    9605
XP_006719576                                                           15    9605
XP_006719575                                                           15    9605
XP_006719574                                                           15    9605
XP_006719573                                                           15    9605
XP_005269086                                                           15    9605
XP_006719572                                                           15    9605
XP_006719571                                                           15    9605
XP_005269085                                                           15    9605
XP_006719570                                                           15    9605
XP_006719569                                                           15    9605
XP_006719568                                                           15    9605
XP_006719567                                                           15    9605
XP_006715101                                                           15    9605
XP_006715100                                                           15    9605
XP_005249021                                                           15    9605
XP_006715099                                                           15    9605
XP_006715098                                                           15    9605
XP_006715097                                                           15    9605
XP_006715096                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           YS SYELIL
Sbjct: 647 YSFSYELIL 655
>NP_001075109
          Length = 4097

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           YS SYELIL
Sbjct: 647 YSFSYELIL 655
>NP_005671
          Length = 588

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           YSLSY+ IL
Sbjct: 535 YSLSYQFIL 543
>NP_116753
          Length = 1037

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           Y+ SYEL+L
Sbjct: 699 YNYSYELVL 707
>NP_116754
          Length = 1048

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           Y+ SYEL+L
Sbjct: 710 YNYSYELVL 718
>NP_001265548
          Length = 1037

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           Y+ SYEL+L
Sbjct: 699 YNYSYELVL 707
>NP_116755
          Length = 1340

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           Y+ SYEL+L
Sbjct: 710 YNYSYELVL 718
>NP_001186480
          Length = 248

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSLSY+L
Sbjct: 179 YSLSYDL 185
>NP_001186552
          Length = 251

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSLSY+L
Sbjct: 182 YSLSYDL 188
>NP_001186481
          Length = 238

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSLSY+L
Sbjct: 169 YSLSYDL 175
>NP_071377
          Length = 238

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSLSY+L
Sbjct: 169 YSLSYDL 175
>XP_005265433
          Length = 164

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSLSY+L
Sbjct: 95  YSLSYDL 101
>XP_005265432
          Length = 164

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSLSY+L
Sbjct: 95  YSLSYDL 101
>XP_005265431
          Length = 238

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSLSY+L
Sbjct: 169 YSLSYDL 175
>NP_002259
          Length = 521

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YSLSYELI 8
           Y L+YE+I
Sbjct: 474 YKLAYEII 481
>NP_001243089
          Length = 543

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YS++YEL
Sbjct: 330 YSINYEL 336
>NP_079273
          Length = 682

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YS++YEL
Sbjct: 330 YSINYEL 336
>NP_006257
          Length = 914

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           YSLS EL++
Sbjct: 525 YSLSRELLI 533
>NP_001035827
          Length = 859

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           YSLS EL++
Sbjct: 470 YSLSRELLI 478
>NP_001258705
          Length = 902

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           YSLS EL++
Sbjct: 513 YSLSRELLI 521
>NP_001258704
          Length = 913

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           YSLS EL++
Sbjct: 524 YSLSRELLI 532
>NP_001258703
          Length = 885

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           YSLS EL++
Sbjct: 496 YSLSRELLI 504
>NP_001001922
          Length = 324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SLSYELIL 9
           SLSY LIL
Sbjct: 224 SLSYTLIL 231
>XP_006721017
          Length = 366

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YS++YEL
Sbjct: 330 YSINYEL 336
>XP_006721016
          Length = 682

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YS++YEL
Sbjct: 330 YSINYEL 336
>NP_055752
          Length = 1132

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSL+Y+L
Sbjct: 178 YSLTYDL 184
>NP_001230124
          Length = 219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSL+Y+L
Sbjct: 178 YSLTYDL 184
>XP_006717783
          Length = 641

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSL+Y+L
Sbjct: 178 YSLTYDL 184
>XP_006717782
          Length = 1089

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YSLSYEL 7
           YSL+Y+L
Sbjct: 135 YSLTYDL 141
>NP_579875
          Length = 436

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  YSLSYELI 8
          YSLS EL+
Sbjct: 20 YSLSRELL 27
>NP_064525
          Length = 414

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 335 NLAYEIIL 342
>NP_002368
          Length = 325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  YSLSYEL 7
          Y+L YEL
Sbjct: 91 YALDYEL 97
>NP_758847
          Length = 425

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  YSLSYELI 8
          YSLS EL+
Sbjct: 20 YSLSRELL 27
>NP_056060
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1045 NLTYEIIL 1052
>NP_001138238
          Length = 438

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YSLSYEL 7
           Y L+YEL
Sbjct: 216 YKLAYEL 222
>NP_000112
          Length = 508

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  YSLSYEL 7
          YS SY+L
Sbjct: 77 YSFSYQL 83
>NP_001191008
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 200 NLAYEIIL 207
>NP_002449
          Length = 5762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   SYELIL 9
           SYELIL
Sbjct: 961 SYELIL 966
>NP_690001
          Length = 1248

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1169 NLAYEIIL 1176
>NP_001191010
          Length = 254

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 175 NLAYEIIL 182
>NP_001191009
          Length = 266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 175 NLAYEIIL 182
>NP_001190994
          Length = 290

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 199 NLAYEIIL 206
>NP_001190996
          Length = 394

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 304 NLAYEIIL 311
>NP_003306
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YSLSYEL 7
           Y L+YEL
Sbjct: 272 YKLAYEL 278
>NP_001190999
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 335 NLAYEIIL 342
>NP_001190998
          Length = 450

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 359 NLAYEIIL 366
>NP_858056
          Length = 510

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 419 NLAYEIIL 426
>NP_001190995
          Length = 357

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 267 NLAYEIIL 274
>NP_001190997
          Length = 460

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 395 NLAYEIIL 402
>NP_002282
          Length = 1786

 Score = 15.4 bits (28), Expect =  9605
 Identities = 3/9 (33%), Positives = 8/9 (88%)

Query: 1   YSLSYELIL 9
           YS+ Y++++
Sbjct: 606 YSMEYDILI 614
>XP_006722117
          Length = 401

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YSLSYEL 7
           Y L+YEL
Sbjct: 160 YKLAYEL 166
>XP_006722116
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YSLSYEL 7
           Y L+YEL
Sbjct: 216 YKLAYEL 222
>XP_006722115
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YSLSYEL 7
           Y L+YEL
Sbjct: 216 YKLAYEL 222
>XP_006722114
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YSLSYEL 7
           Y L+YEL
Sbjct: 216 YKLAYEL 222
>XP_006722113
          Length = 513

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YSLSYEL 7
           Y L+YEL
Sbjct: 272 YKLAYEL 278
>XP_005269089
          Length = 266

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLSYELIL 9
           +L+YE+IL
Sbjct: 175 NLAYEIIL 182
>XP_006719577
          Length = 1184

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1105 NLAYEIIL 1112
>XP_006719576
          Length = 1199

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1109 NLAYEIIL 1116
>XP_006719575
          Length = 1200

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1109 NLAYEIIL 1116
>XP_006719574
          Length = 1224

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1133 NLAYEIIL 1140
>XP_006719573
          Length = 1235

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1169 NLAYEIIL 1176
>XP_005269086
          Length = 1256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1165 NLAYEIIL 1172
>XP_006719572
          Length = 1259

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1169 NLAYEIIL 1176
>XP_006719571
          Length = 1260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1194 NLAYEIIL 1201
>XP_005269085
          Length = 1260

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1169 NLAYEIIL 1176
>XP_006719570
          Length = 1273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1194 NLAYEIIL 1201
>XP_006719569
          Length = 1283

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1193 NLAYEIIL 1200
>XP_006719568
          Length = 1284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1194 NLAYEIIL 1201
>XP_006719567
          Length = 1284

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1193 NLAYEIIL 1200
>XP_006715101
          Length = 1150

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1045 NLTYEIIL 1052
>XP_006715100
          Length = 1151

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1046 NLTYEIIL 1053
>XP_005249021
          Length = 1155

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1066 NLTYEIIL 1073
>XP_006715099
          Length = 1156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1067 NLTYEIIL 1074
>XP_006715098
          Length = 1161

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1066 NLTYEIIL 1073
>XP_006715097
          Length = 1171

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1066 NLTYEIIL 1073
>XP_006715096
          Length = 1172

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SLSYELIL 9
            +L+YE+IL
Sbjct: 1067 NLTYEIIL 1074
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.323    0.143    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 522,500
Number of extensions: 864
Number of successful extensions: 76
Number of sequences better than 10000.0: 76
Number of HSP's gapped: 76
Number of HSP's successfully gapped: 76
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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