Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              20     299
XP_005251512                                                           20     299
NP_112598                                                              17    2528
NP_077305                                                              17    4312
NP_001037729                                                           16    5631
NP_872353                                                              16    5631
NP_059980                                                              16    5631
NP_055230                                                              16    5631
NP_001278220                                                           16    7355
NP_037428                                                              16    7355
XP_006710652                                                           16    7355
XP_006710651                                                           16    7355
XP_005270844                                                           16    7355
NP_000531                                                              15    9605
NP_001036188                                                           15    9605
NP_002218                                                              15    9605
NP_008870                                                              15    9605
NP_001271260                                                           15    9605
NP_001615                                                              15    9605
NP_954652                                                              15    9605
NP_001230181                                                           15    9605
NP_079478                                                              15    9605
NP_003128                                                              15    9605
XP_006725233                                                           15    9605
XP_006725232                                                           15    9605
XP_006725231                                                           15    9605
XP_006723382                                                           15    9605
XP_006723381                                                           15    9605
XP_006723380                                                           15    9605
XP_005268078                                                           15    9605
XP_006720285                                                           15    9605
XP_005267988                                                           15    9605
XP_005267987                                                           15    9605
XP_005250608                                                           15    9605
XP_006716161                                                           15    9605
XP_005250607                                                           15    9605
XP_005250606                                                           15    9605
XP_005266896                                                           15    9605
XP_005270898                                                           15    9605
XP_006710687                                                           15    9605
NP_478144                                                              15    9605
NP_037416                                                              15    9605
>NP_004963
          Length = 1132

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   YSKSFFEAA 9
           YS+SFFEAA
Sbjct: 590 YSESFFEAA 598
>XP_005251512
          Length = 1132

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   YSKSFFEAA 9
           YS+SFFEAA
Sbjct: 590 YSESFFEAA 598
>NP_112598
          Length = 5088

 Score = 17.3 bits (33), Expect =  2528
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YSKSFFEAA 9
           + KSFF+AA
Sbjct: 297 HKKSFFQAA 305
>NP_077305
          Length = 240

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKSFFEA 8
           +KSFFEA
Sbjct: 190 AKSFFEA 196
>NP_001037729
          Length = 139

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  YSKSFFEA 8
          YSK +F+A
Sbjct: 35 YSKCYFQA 42
>NP_872353
          Length = 227

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KSFFEA 8
           KSFFEA
Sbjct: 218 KSFFEA 223
>NP_059980
          Length = 235

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KSFFEA 8
           KSFFEA
Sbjct: 226 KSFFEA 231
>NP_055230
          Length = 1053

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   KSFFEA 8
           KSFFEA
Sbjct: 773 KSFFEA 778
>NP_001278220
          Length = 219

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   KSFFEAA 9
           K FFEAA
Sbjct: 100 KRFFEAA 106
>NP_037428
          Length = 684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SFFEAA 9
          SFFEAA
Sbjct: 45 SFFEAA 50
>XP_006710652
          Length = 665

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SFFEAA 9
          SFFEAA
Sbjct: 26 SFFEAA 31
>XP_006710651
          Length = 684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SFFEAA 9
          SFFEAA
Sbjct: 45 SFFEAA 50
>XP_005270844
          Length = 684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  SFFEAA 9
          SFFEAA
Sbjct: 45 SFFEAA 50
>NP_000531
          Length = 5038

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    KSFFEAA 9
            +SFFE+A
Sbjct: 3093 RSFFESA 3099
>NP_001036188
          Length = 5033

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    KSFFEAA 9
            +SFFE+A
Sbjct: 3093 RSFFESA 3099
>NP_002218
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKSFFEAA 9
           S +FFEAA
Sbjct: 632 SLAFFEAA 639
>NP_008870
          Length = 1332

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    YSKSFFEA 8
            +SKSFF +
Sbjct: 1125 HSKSFFSS 1132
>NP_001271260
          Length = 457

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFFEAA 9
           K+FF+AA
Sbjct: 430 KTFFQAA 436
>NP_001615
          Length = 1723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1    YSKSFFE 7
            Y K FFE
Sbjct: 1223 YEKPFFE 1229
>NP_954652
          Length = 228

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   YSKSFF 6
           Y KSFF
Sbjct: 123 YKKSFF 128
>NP_001230181
          Length = 143

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SKSFFEA 8
          +K+FFEA
Sbjct: 92 AKNFFEA 98
>NP_079478
          Length = 239

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SKSFFEA 8
           +K+FFEA
Sbjct: 188 AKNFFEA 194
>NP_003128
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   YSKSFF 6
           YSK FF
Sbjct: 586 YSKEFF 591
>XP_006725233
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSKSFF 6
           +SKSFF
Sbjct: 490 FSKSFF 495
>XP_006725232
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSKSFF 6
           +SKSFF
Sbjct: 490 FSKSFF 495
>XP_006725231
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSKSFF 6
           +SKSFF
Sbjct: 490 FSKSFF 495
>XP_006723382
          Length = 5027

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    KSFFEAA 9
            +SFFE+A
Sbjct: 3093 RSFFESA 3099
>XP_006723381
          Length = 5031

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    KSFFEAA 9
            +SFFE+A
Sbjct: 3092 RSFFESA 3098
>XP_006723380
          Length = 5032

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3    KSFFEAA 9
            +SFFE+A
Sbjct: 3093 RSFFESA 3099
>XP_005268078
          Length = 1272

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    YSKSFFEA 8
            +SKSFF +
Sbjct: 1065 HSKSFFSS 1072
>XP_006720285
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSKSFF 6
           +SKSFF
Sbjct: 490 FSKSFF 495
>XP_005267988
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSKSFF 6
           +SKSFF
Sbjct: 490 FSKSFF 495
>XP_005267987
          Length = 766

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSKSFF 6
           +SKSFF
Sbjct: 490 FSKSFF 495
>XP_005250608
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   YSKSFF 6
           YSK FF
Sbjct: 650 YSKEFF 655
>XP_006716161
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   YSKSFF 6
           YSK FF
Sbjct: 650 YSKEFF 655
>XP_005250607
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   YSKSFF 6
           YSK FF
Sbjct: 661 YSKEFF 666
>XP_005250606
          Length = 767

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   YSKSFF 6
           YSK FF
Sbjct: 698 YSKEFF 703
>XP_005266896
          Length = 2131

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1    YSKSFFE 7
            Y K FFE
Sbjct: 1631 YEKPFFE 1637
>XP_005270898
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKSFFEAA 9
           S +FFEAA
Sbjct: 632 SLAFFEAA 639
>XP_006710687
          Length = 1154

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SKSFFEAA 9
           S +FFEAA
Sbjct: 632 SLAFFEAA 639
>NP_478144
          Length = 873

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YSKSFF 6
           +SKSFF
Sbjct: 597 FSKSFF 602
>NP_037416
          Length = 494

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSFFEAA 9
           K+FF+AA
Sbjct: 467 KTFFQAA 473
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.124    0.335 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 425,481
Number of extensions: 720
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

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