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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001230688                                                           16    5631
NP_004196                                                              16    5631
NP_741994                                                              16    5631
XP_005271779                                                           16    5631
XP_005271778                                                           16    5631
NP_775754                                                              16    7355
NP_525021                                                              16    7355
NP_115971                                                              16    7355
NP_004496                                                              16    7355
XP_005256902                                                           16    7355
NP_835454                                                              15    9605
NP_073617                                                              15    9605

>NP_001230688
          Length = 362

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YRLYAVS 7
           Y LYAVS
Sbjct: 298 YNLYAVS 304



>NP_004196
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YRLYAVS 7
           Y LYAVS
Sbjct: 541 YNLYAVS 547



>NP_741994
          Length = 396

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YRLYAVS 7
           Y LYAVS
Sbjct: 332 YNLYAVS 338



>XP_005271779
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YRLYAVS 7
           Y LYAVS
Sbjct: 541 YNLYAVS 547



>XP_005271778
          Length = 605

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YRLYAVS 7
           Y LYAVS
Sbjct: 541 YNLYAVS 547



>NP_775754
          Length = 531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1  YRLYAVS 7
          +R+YAVS
Sbjct: 19 FRIYAVS 25



>NP_525021
          Length = 1543

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YRLYAVSLV 9
           Y LY +SL+
Sbjct: 217 YSLYGLSLI 225



>NP_115971
          Length = 1604

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    YRLYAVS 7
            Y LYA+S
Sbjct: 1510 YNLYAIS 1516



>NP_004496
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    YRLYAVS 7
            Y LYA+S
Sbjct: 1312 YNLYAIS 1318



>XP_005256902
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    YRLYAVS 7
            Y LYA+S
Sbjct: 1312 YNLYAIS 1318



>NP_835454
          Length = 562

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YRLYAVS 7
           YR++AVS
Sbjct: 547 YRMHAVS 553



>NP_073617
          Length = 391

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YRLYAVSLV 9
           +RLY +SL+
Sbjct: 195 HRLYFISLL 203



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.337    0.146    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 363,599
Number of extensions: 441
Number of successful extensions: 12
Number of sequences better than 10000.0: 12
Number of HSP's gapped: 12
Number of HSP's successfully gapped: 12
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)