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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_596870                                                              17    3301
NP_060102                                                              16    5631
NP_149099                                                              16    7355
NP_056153                                                              16    7355
XP_005262622                                                           16    7355
XP_005262565                                                           16    7355
XP_006723795                                                           16    7355
XP_006723794                                                           16    7355
XP_006723793                                                           16    7355
XP_006723792                                                           16    7355
XP_006723791                                                           16    7355
XP_006723790                                                           16    7355
NP_714927                                                              16    7355
NP_001191339                                                           15    9605
NP_002994                                                              15    9605
NP_001007076                                                           15    9605
NP_057074                                                              15    9605
NP_950240                                                              15    9605
NP_001137473                                                           15    9605
NP_001137471                                                           15    9605
NP_001137470                                                           15    9605
NP_001191337                                                           15    9605
NP_835224                                                              15    9605
NP_056173                                                              15    9605
NP_001034662                                                           15    9605
XP_006724049                                                           15    9605
XP_006724048                                                           15    9605
XP_005262712                                                           15    9605
XP_005262711                                                           15    9605
XP_005247001                                                           15    9605
NP_005006                                                              15    9605
NP_001929                                                              15    9605

>NP_596870
          Length = 5604

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    YRASSQMEN 9
            YR +SQ EN
Sbjct: 4253 YRENSQFEN 4261



>NP_060102
          Length = 580

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RASSQMEN 9
           RAS+Q EN
Sbjct: 167 RASAQQEN 174



>NP_149099
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           +RA + MEN
Sbjct: 216 FRAGANMEN 224



>NP_056153
          Length = 1541

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YRASSQ 6
           YRASSQ
Sbjct: 152 YRASSQ 157



>XP_005262622
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           +RA + MEN
Sbjct: 138 FRAGANMEN 146



>XP_005262565
          Length = 323

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           +RA + MEN
Sbjct: 138 FRAGANMEN 146



>XP_006723795
          Length = 1480

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YRASSQ 6
           YRASSQ
Sbjct: 142 YRASSQ 147



>XP_006723794
          Length = 1490

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YRASSQ 6
           YRASSQ
Sbjct: 152 YRASSQ 157



>XP_006723793
          Length = 1513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YRASSQ 6
           YRASSQ
Sbjct: 124 YRASSQ 129



>XP_006723792
          Length = 1513

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YRASSQ 6
           YRASSQ
Sbjct: 124 YRASSQ 129



>XP_006723791
          Length = 1531

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YRASSQ 6
           YRASSQ
Sbjct: 142 YRASSQ 147



>XP_006723790
          Length = 1540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YRASSQ 6
           YRASSQ
Sbjct: 151 YRASSQ 156



>NP_714927
          Length = 401

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           +RA + MEN
Sbjct: 216 FRAGANMEN 224



>NP_001191339
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           YR + ++EN
Sbjct: 506 YRTAEELEN 514



>NP_002994
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           YR + ++EN
Sbjct: 506 YRTAEELEN 514



>NP_001007076
          Length = 709

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  YRASSQME 8
          YR SSQ++
Sbjct: 68 YRRSSQLD 75



>NP_057074
          Length = 755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YRASSQME 8
           YR SSQ++
Sbjct: 114 YRRSSQLD 121



>NP_950240
          Length = 694

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YRASSQME 8
           YR SSQ++
Sbjct: 114 YRRSSQLD 121



>NP_001137473
          Length = 681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           YR + ++EN
Sbjct: 472 YRTAEELEN 480



>NP_001137471
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           YR + ++EN
Sbjct: 506 YRTAEELEN 514



>NP_001137470
          Length = 715

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           YR + ++EN
Sbjct: 506 YRTAEELEN 514



>NP_001191337
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           YR + ++EN
Sbjct: 506 YRTAEELEN 514



>NP_835224
          Length = 696

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YRASSQME 8
           YR +SQ+E
Sbjct: 364 YRQASQLE 371



>NP_056173
          Length = 939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RASSQMEN 9
           R SSQ EN
Sbjct: 558 RLSSQFEN 565



>NP_001034662
          Length = 719

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   YRASSQMEN 9
           YR + ++EN
Sbjct: 506 YRTAEELEN 514



>XP_006724049
          Length = 978

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RASSQMEN 9
           R SSQ EN
Sbjct: 597 RLSSQFEN 604



>XP_006724048
          Length = 868

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   RASSQMEN 9
           R SSQ EN
Sbjct: 487 RLSSQFEN 494



>XP_005262712
          Length = 789

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YRASSQME 8
           YR SSQ++
Sbjct: 148 YRRSSQLD 155



>XP_005262711
          Length = 791

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YRASSQME 8
           YR SSQ++
Sbjct: 148 YRRSSQLD 155



>XP_005247001
          Length = 655

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YRASSQME 8
           YR +SQ+E
Sbjct: 364 YRQASQLE 371



>NP_005006
          Length = 495

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YRASSQM 7
           Y+ASS+M
Sbjct: 256 YKASSEM 262



>NP_001929
          Length = 176

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RASSQMEN 9
           +ASS++EN
Sbjct: 103 KASSRLEN 110



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.299    0.105    0.256 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 327,629
Number of extensions: 371
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)