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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001240775                                                           16    7355
NP_001491                                                              16    7355
NP_055426                                                              16    7355
NP_004648                                                              16    7355
XP_005248757                                                           16    7355
XP_006715468                                                           16    7355
XP_005248756                                                           16    7355
XP_006715467                                                           16    7355
XP_006715129                                                           16    7355

>NP_001240775
          Length = 342

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YQASTS 6
           YQASTS
Sbjct: 121 YQASTS 126



>NP_001491
          Length = 372

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YQASTS 6
           YQASTS
Sbjct: 151 YQASTS 156



>NP_055426
          Length = 5596

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YQASTSLVT 9
            YQ   SLVT
Sbjct: 3660 YQTGASLVT 3668



>NP_004648
          Length = 425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YQASTS 6
           YQASTS
Sbjct: 103 YQASTS 108



>XP_005248757
          Length = 3591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YQASTSLVT 9
            YQ   SLVT
Sbjct: 1655 YQTGASLVT 1663



>XP_006715468
          Length = 3744

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YQASTSLVT 9
            YQ   SLVT
Sbjct: 1808 YQTGASLVT 1816



>XP_005248756
          Length = 3905

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YQASTSLVT 9
            YQ   SLVT
Sbjct: 1969 YQTGASLVT 1977



>XP_006715467
          Length = 5550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YQASTSLVT 9
            YQ   SLVT
Sbjct: 3660 YQTGASLVT 3668



>XP_006715129
          Length = 290

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YQASTS 6
           YQASTS
Sbjct: 151 YQASTS 156



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.302    0.106    0.251 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 242,127
Number of extensions: 285
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)