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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              20     509
XP_005265476                                                           16    7355
NP_003232                                                              16    7355
NP_212134                                                              15    9605
NP_671492                                                              15    9605
NP_001004065                                                           15    9605
NP_001185585                                                           15    9605
NP_009134                                                              15    9605
XP_006723601                                                           15    9605
XP_005260712                                                           15    9605
NP_001130034                                                           15    9605

>NP_073713
          Length = 985

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   YNSRQAVMS 9
           Y+SRQAVMS
Sbjct: 92  YDSRQAVMS 100



>XP_005265476
          Length = 601

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YNSRQAV 7
           YN++QAV
Sbjct: 174 YNTKQAV 180



>NP_003232
          Length = 684

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YNSRQAV 7
           YN++QAV
Sbjct: 257 YNTKQAV 263



>NP_212134
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   YNSRQAVMS 9
           YN   AVMS
Sbjct: 376 YNMAMAVMS 384



>NP_671492
          Length = 1090

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NSRQAV 7
           NSRQAV
Sbjct: 566 NSRQAV 571



>NP_001004065
          Length = 948

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NSRQAV 7
           NSRQAV
Sbjct: 424 NSRQAV 429



>NP_001185585
          Length = 961

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NSRQAV 7
           NSRQAV
Sbjct: 424 NSRQAV 429



>NP_009134
          Length = 1103

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NSRQAV 7
           NSRQAV
Sbjct: 566 NSRQAV 571



>XP_006723601
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   YNSRQAVMS 9
           YN   AVMS
Sbjct: 376 YNMAMAVMS 384



>XP_005260712
          Length = 469

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   YNSRQAVMS 9
           YN   AVMS
Sbjct: 376 YNMAMAVMS 384



>NP_001130034
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   NSRQAV 7
           NSRQAV
Sbjct: 335 NSRQAV 340



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.312    0.111    0.278 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 283,620
Number of extensions: 89
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)