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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           18    1135
NP_036462                                                              18    1135
NP_001243397                                                           18    1135
XP_005269721                                                           18    1135
NP_003957                                                              17    3301
XP_006714571                                                           17    3301
XP_006714570                                                           17    3301
XP_006714569                                                           17    3301
NP_997204                                                              17    4312
NP_001127880                                                           16    5631
NP_000824                                                              16    5631
NP_001127879                                                           16    5631
NP_001070894                                                           16    5631
XP_005255325                                                           16    5631
XP_005255324                                                           16    5631
NP_848622                                                              15    9605

>NP_001243398
          Length = 1781

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MRTKSASL 9
            MRTKSASL
Sbjct: 1633 MRTKSASL 1640



>NP_036462
          Length = 2073

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MRTKSASL 9
            MRTKSASL
Sbjct: 1925 MRTKSASL 1932



>NP_001243397
          Length = 1890

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MRTKSASL 9
            MRTKSASL
Sbjct: 1742 MRTKSASL 1749



>XP_005269721
          Length = 2073

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    MRTKSASL 9
            MRTKSASL
Sbjct: 1925 MRTKSASL 1932



>NP_003957
          Length = 1074

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   YMRTKSAS 8
           YMRT+S S
Sbjct: 862 YMRTRSCS 869



>XP_006714571
          Length = 917

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   YMRTKSAS 8
           YMRT+S S
Sbjct: 705 YMRTRSCS 712



>XP_006714570
          Length = 1074

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   YMRTKSAS 8
           YMRT+S S
Sbjct: 862 YMRTRSCS 869



>XP_006714569
          Length = 1074

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   YMRTKSAS 8
           YMRT+S S
Sbjct: 862 YMRTRSCS 869



>NP_997204
          Length = 480

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1   YMRTKS 6
           YMRTKS
Sbjct: 212 YMRTKS 217



>NP_001127880
          Length = 1281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    YMRTKSAS 8
            Y++TKS+S
Sbjct: 1105 YLKTKSSS 1112



>NP_000824
          Length = 1464

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    YMRTKSAS 8
            Y++TKS+S
Sbjct: 1105 YLKTKSSS 1112



>NP_001127879
          Length = 1464

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    YMRTKSAS 8
            Y++TKS+S
Sbjct: 1105 YLKTKSSS 1112



>NP_001070894
          Length = 210

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  YMRTKSASL 9
          Y+RT  ASL
Sbjct: 54 YLRTSPASL 62



>XP_005255325
          Length = 1333

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    YMRTKSAS 8
            Y++TKS+S
Sbjct: 1157 YLKTKSSS 1164



>XP_005255324
          Length = 1516

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1    YMRTKSAS 8
            Y++TKS+S
Sbjct: 1157 YLKTKSSS 1164



>NP_848622
          Length = 1124

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   YMRTKS 6
           YMRT+S
Sbjct: 562 YMRTRS 567



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.315    0.114    0.282 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 265,510
Number of extensions: 293
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)