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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_055365                                                              20     509
NP_001258358                                                           17    4312
NP_001258357                                                           17    4312
NP_055862                                                              17    4312
NP_001258356                                                           17    4312
XP_005245845                                                           17    4312
XP_005245844                                                           17    4312
NP_001180584                                                           16    5631
NP_001180586                                                           16    5631
NP_001180583                                                           16    5631
NP_001093877                                                           16    5631
NP_001180582                                                           16    5631
NP_001028218                                                           16    5631
NP_001093878                                                           16    5631
XP_006722356                                                           16    5631
NP_060626                                                              15    9605
NP_056193                                                              15    9605

>NP_055365
          Length = 1032

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   YMITMRLAQ 9
           YMI MRLAQ
Sbjct: 207 YMIAMRLAQ 215



>NP_001258358
          Length = 971

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MITMRLAQ 9
           MIT RLAQ
Sbjct: 955 MITKRLAQ 962



>NP_001258357
          Length = 992

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MITMRLAQ 9
           MIT RLAQ
Sbjct: 976 MITKRLAQ 983



>NP_055862
          Length = 993

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MITMRLAQ 9
           MIT RLAQ
Sbjct: 977 MITKRLAQ 984



>NP_001258356
          Length = 992

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MITMRLAQ 9
           MIT RLAQ
Sbjct: 976 MITKRLAQ 983



>XP_005245845
          Length = 995

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MITMRLAQ 9
           MIT RLAQ
Sbjct: 979 MITKRLAQ 986



>XP_005245844
          Length = 996

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   MITMRLAQ 9
           MIT RLAQ
Sbjct: 980 MITKRLAQ 987



>NP_001180584
          Length = 187

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YMITMRLA 8
           YM+ +RLA
Sbjct: 124 YMVVLRLA 131



>NP_001180586
          Length = 187

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YMITMRLA 8
           YM+ +RLA
Sbjct: 124 YMVVLRLA 131



>NP_001180583
          Length = 187

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YMITMRLA 8
           YM+ +RLA
Sbjct: 124 YMVVLRLA 131



>NP_001093877
          Length = 187

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YMITMRLA 8
           YM+ +RLA
Sbjct: 124 YMVVLRLA 131



>NP_001180582
          Length = 187

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YMITMRLA 8
           YM+ +RLA
Sbjct: 124 YMVVLRLA 131



>NP_001028218
          Length = 187

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YMITMRLA 8
           YM+ +RLA
Sbjct: 124 YMVVLRLA 131



>NP_001093878
          Length = 173

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YMITMRLA 8
           YM+ +RLA
Sbjct: 110 YMVVLRLA 117



>XP_006722356
          Length = 173

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YMITMRLA 8
           YM+ +RLA
Sbjct: 110 YMVVLRLA 117



>NP_060626
          Length = 4363

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    YMITMRL 7
            YM+ MRL
Sbjct: 3223 YMVRMRL 3229



>NP_056193
          Length = 4388

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    YMITMRL 7
            YM+ MRL
Sbjct: 3248 YMVRMRL 3254



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.345    0.138    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 229,732
Number of extensions: 181
Number of successful extensions: 17
Number of sequences better than 10000.0: 17
Number of HSP's gapped: 17
Number of HSP's successfully gapped: 17
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.8 bits)
S2: 28 (15.4 bits)