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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              18    1135
NP_001075109                                                           18    1135
NP_078961                                                              17    4312
XP_006719666                                                           17    4312
NP_996555                                                              15    9605
NP_001241649                                                           15    9605
NP_001241647                                                           15    9605
NP_001241650                                                           15    9605
NP_001241652                                                           15    9605
NP_002456                                                              15    9605
NP_001241651                                                           15    9605
NP_996557                                                              15    9605
NP_001241648                                                           15    9605
NP_996556                                                              15    9605
XP_006719474                                                           15    9605
XP_005268933                                                           15    9605
XP_006719473                                                           15    9605
XP_006719472                                                           15    9605
XP_006719471                                                           15    9605
XP_006719470                                                           15    9605
XP_006719469                                                           15    9605
XP_006719468                                                           15    9605

>NP_008835
          Length = 4128

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    YMIIANALS 9
            Y IIANALS
Sbjct: 3334 YRIIANALS 3342



>NP_001075109
          Length = 4097

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    YMIIANALS 9
            Y IIANALS
Sbjct: 3334 YRIIANALS 3342



>NP_078961
          Length = 723

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   MIIANAL 8
           MIIANAL
Sbjct: 631 MIIANAL 637



>XP_006719666
          Length = 657

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 2   MIIANAL 8
           MIIANAL
Sbjct: 565 MIIANAL 571



>NP_996555
          Length = 1148

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 513 LVIANALT 520



>NP_001241649
          Length = 1134

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 476 LVIANALT 483



>NP_001241647
          Length = 1173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 488 LVIANALT 495



>NP_001241650
          Length = 1127

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 469 LVIANALT 476



>NP_001241652
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 475 LVIANALT 482



>NP_002456
          Length = 1171

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 513 LVIANALT 520



>NP_001241651
          Length = 1120

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 462 LVIANALT 469



>NP_996557
          Length = 1123

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 488 LVIANALT 495



>NP_001241648
          Length = 1146

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 488 LVIANALT 495



>NP_996556
          Length = 1141

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 488 LVIANALT 495



>XP_006719474
          Length = 1164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 488 LVIANALT 495



>XP_005268933
          Length = 1165

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 501 LVIANALT 508



>XP_006719473
          Length = 1166

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 513 LVIANALT 520



>XP_006719472
          Length = 1170

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 488 LVIANALT 495



>XP_006719471
          Length = 1177

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 513 LVIANALT 520



>XP_006719470
          Length = 1183

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 501 LVIANALT 508



>XP_006719469
          Length = 1189

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 513 LVIANALT 520



>XP_006719468
          Length = 1195

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   MIIANALS 9
           ++IANAL+
Sbjct: 513 LVIANALT 520



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.134    0.343 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 203,962
Number of extensions: 426
Number of successful extensions: 22
Number of sequences better than 10000.0: 22
Number of HSP's gapped: 22
Number of HSP's successfully gapped: 22
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)