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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036462                                                              18    1482
NP_001243397                                                           18    1482
XP_005269721                                                           18    1482
XP_005274178                                                           17    4312
XP_005274177                                                           17    4312
XP_005274175                                                           17    4312
NP_065731                                                              17    4312
NP_001041683                                                           17    4312
NP_001180569                                                           16    7355
NP_957705                                                              16    7355
NP_001244894                                                           16    7355
NP_001138498                                                           16    7355
NP_112196                                                              16    7355
NP_001189352                                                           16    7355
XP_006711313                                                           16    7355
XP_006711312                                                           16    7355
NP_073747                                                              15    9605
NP_000483                                                              15    9605
XP_006715905                                                           15    9605
XP_005271187                                                           15    9605

>NP_036462
          Length = 2073

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   YISASTRKV 9
           YISAST KV
Sbjct: 419 YISASTLKV 427



>NP_001243397
          Length = 1890

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   YISASTRKV 9
           YISAST KV
Sbjct: 419 YISASTLKV 427



>XP_005269721
          Length = 2073

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   YISASTRKV 9
           YISAST KV
Sbjct: 419 YISASTLKV 427



>XP_005274178
          Length = 780

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YISASTR 7
           Y+SASTR
Sbjct: 458 YLSASTR 464



>XP_005274177
          Length = 781

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YISASTR 7
           Y+SASTR
Sbjct: 458 YLSASTR 464



>XP_005274175
          Length = 807

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YISASTR 7
           Y+SASTR
Sbjct: 458 YLSASTR 464



>NP_065731
          Length = 808

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YISASTR 7
           Y+SASTR
Sbjct: 458 YLSASTR 464



>NP_001041683
          Length = 791

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   YISASTR 7
           Y+SASTR
Sbjct: 458 YLSASTR 464



>NP_001180569
          Length = 1294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YISASTRKV 9
           +ISAST K+
Sbjct: 704 FISASTWKI 712



>NP_957705
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YISASTRKV 9
           +ISAST K+
Sbjct: 816 FISASTWKI 824



>NP_001244894
          Length = 1382

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YISASTRKV 9
           +ISAST K+
Sbjct: 747 FISASTWKI 755



>NP_001138498
          Length = 2299

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   YISASTR 7
           Y++ASTR
Sbjct: 922 YVTASTR 928



>NP_112196
          Length = 733

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   YISASTRK 8
           YI+AST K
Sbjct: 216 YIAASTAK 223



>NP_001189352
          Length = 577

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  YISASTRK 8
          YI+AST K
Sbjct: 60 YIAASTAK 67



>XP_006711313
          Length = 942

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YISASTRKV 9
           +ISAST K+
Sbjct: 465 FISASTWKI 473



>XP_006711312
          Length = 1100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   YISASTRKV 9
           +ISAST K+
Sbjct: 510 FISASTWKI 518



>NP_073747
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   YISASTRK 8
           Y+S+S+RK
Sbjct: 326 YMSSSSRK 333



>NP_000483
          Length = 1480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASTRKV 9
           +ASTRKV
Sbjct: 788 TASTRKV 794



>XP_006715905
          Length = 1588

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SASTRKV 9
           +ASTRKV
Sbjct: 896 TASTRKV 902



>XP_005271187
          Length = 406

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   YISASTRK 8
           Y+S+S+RK
Sbjct: 200 YMSSSSRK 207



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.118    0.288 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 286,059
Number of extensions: 378
Number of successful extensions: 20
Number of sequences better than 10000.0: 20
Number of HSP's gapped: 20
Number of HSP's successfully gapped: 20
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)