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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_064450                                                              20     509
XP_005273257                                                           20     509
NP_878917                                                              16    5631
NP_878918                                                              16    5631
NP_057196                                                              16    5631
NP_001240766                                                           16    5631
XP_005267517                                                           16    5631
XP_005267514                                                           16    5631
XP_005267513                                                           16    5631
XP_005267512                                                           16    5631
XP_005267511                                                           16    5631
NP_055177                                                              16    7355
NP_932164                                                              16    7355
NP_005914                                                              15    9605
NP_001268451                                                           15    9605
NP_001268449                                                           15    9605
NP_877954                                                              15    9605
NP_001268450                                                           15    9605
NP_115874                                                              15    9605
NP_001013863                                                           15    9605
NP_060190                                                              15    9605
XP_005259649                                                           15    9605
XP_006722854                                                           15    9605
XP_006722853                                                           15    9605
XP_005258138                                                           15    9605
XP_005267049                                                           15    9605
XP_005267047                                                           15    9605
XP_006715545                                                           15    9605
XP_005267048                                                           15    9605
NP_001129955                                                           15    9605

>NP_064450
          Length = 1722

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   YIKITTRQL 9
           YIK TTRQL
Sbjct: 418 YIKTTTRQL 426



>XP_005273257
          Length = 1726

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   YIKITTRQL 9
           YIK TTRQL
Sbjct: 418 YIKTTTRQL 426



>NP_878917
          Length = 556

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  YIKITTRQL 9
          Y+K+TT+ L
Sbjct: 89 YLKMTTKTL 97



>NP_878918
          Length = 6907

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    YIKITTRQL 9
            Y+K+TT+ L
Sbjct: 6455 YLKMTTKTL 6463



>NP_057196
          Length = 805

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YIKITTRQL 9
           YIK T R+L
Sbjct: 96  YIKTTLREL 104



>NP_001240766
          Length = 758

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  YIKITTRQL 9
          YIK T R+L
Sbjct: 49 YIKTTLREL 57



>XP_005267517
          Length = 6883

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    YIKITTRQL 9
            Y+K+TT+ L
Sbjct: 6416 YLKMTTKTL 6424



>XP_005267514
          Length = 6908

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    YIKITTRQL 9
            Y+K+TT+ L
Sbjct: 6455 YLKMTTKTL 6463



>XP_005267513
          Length = 6921

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    YIKITTRQL 9
            Y+K+TT+ L
Sbjct: 6455 YLKMTTKTL 6463



>XP_005267512
          Length = 6922

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    YIKITTRQL 9
            Y+K+TT+ L
Sbjct: 6455 YLKMTTKTL 6463



>XP_005267511
          Length = 6922

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1    YIKITTRQL 9
            Y+K+TT+ L
Sbjct: 6455 YLKMTTKTL 6463



>NP_055177
          Length = 386

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IKITTRQL 9
           +KIT RQL
Sbjct: 307 LKITLRQL 314



>NP_932164
          Length = 338

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IKITTRQL 9
           +KIT RQL
Sbjct: 307 LKITLRQL 314



>NP_005914
          Length = 1374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   YIKITTRQ 8
           ++K+TT Q
Sbjct: 524 FVKLTTEQ 531



>NP_001268451
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ITTRQL 9
           ITTRQL
Sbjct: 661 ITTRQL 666



>NP_001268449
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ITTRQL 9
           ITTRQL
Sbjct: 708 ITTRQL 713



>NP_877954
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ITTRQL 9
           ITTRQL
Sbjct: 692 ITTRQL 697



>NP_001268450
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ITTRQL 9
           ITTRQL
Sbjct: 748 ITTRQL 753



>NP_115874
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   ITTRQL 9
           ITTRQL
Sbjct: 708 ITTRQL 713



>NP_001013863
          Length = 403

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IKITTRQL 9
           + +TTRQ+
Sbjct: 187 VSVTTRQM 194



>NP_060190
          Length = 449

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IKITTRQL 9
           + +TTRQ+
Sbjct: 187 VSVTTRQM 194



>XP_005259649
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IKITTRQL 9
           + +TTRQ+
Sbjct: 264 VSVTTRQM 271



>XP_006722854
          Length = 525

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IKITTRQL 9
           + +TTRQ+
Sbjct: 264 VSVTTRQM 271



>XP_006722853
          Length = 526

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   IKITTRQL 9
           + +TTRQ+
Sbjct: 264 VSVTTRQM 271



>XP_005258138
          Length = 337

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  YIKITTRQL 9
          Y+K T RQ+
Sbjct: 15 YLKATGRQM 23



>XP_005267049
          Length = 808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   YIKITTRQ 8
           ++K+TT Q
Sbjct: 633 FVKLTTEQ 640



>XP_005267047
          Length = 1448

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   YIKITTRQ 8
           ++K+TT Q
Sbjct: 633 FVKLTTEQ 640



>XP_006715545
          Length = 1483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   YIKITTRQ 8
           ++K+TT Q
Sbjct: 633 FVKLTTEQ 640



>XP_005267048
          Length = 1484

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   YIKITTRQ 8
           ++K+TT Q
Sbjct: 633 FVKLTTEQ 640



>NP_001129955
          Length = 164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YIKITTRQL 9
           YI+ TTR L
Sbjct: 151 YIRNTTRYL 159



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.142    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 350,033
Number of extensions: 480
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)