Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006209                                                              21     175
XP_006713721                                                           21     175
NP_002477                                                              17    3301
XP_005252054                                                           17    3301
NP_003801                                                              17    4312
NP_620168                                                              17    4312
NP_056312                                                              17    4312
NP_001273416                                                           17    4312
NP_001171473                                                           17    4312
XP_006722720                                                           17    4312
XP_006722719                                                           17    4312
XP_006722718                                                           17    4312
XP_006722717                                                           17    4312
XP_006720531                                                           17    4312
XP_005254344                                                           17    4312
NP_001005499                                                           16    5631
NP_001230396                                                           16    5631
NP_061834                                                              16    5631
NP_689646                                                              16    5631
NP_940841                                                              16    5631
NP_001001895                                                           16    5631
XP_006724076                                                           16    5631
XP_006718830                                                           16    5631
NP_001138432                                                           16    5631
NP_001035521                                                           16    7355
NP_001004730                                                           16    7355
>NP_006209
          Length = 1068

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  YIFISVTQE 9
          YIF+SVTQE
Sbjct: 68 YIFVSVTQE 76
>XP_006713721
          Length = 1068

 Score = 21.2 bits (43), Expect =   175
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  YIFISVTQE 9
          YIF+SVTQE
Sbjct: 68 YIFVSVTQE 76
>NP_002477
          Length = 790

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   FISVTQE 9
           F+SVTQE
Sbjct: 152 FVSVTQE 158
>XP_005252054
          Length = 723

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3  FISVTQE 9
          F+SVTQE
Sbjct: 85 FVSVTQE 91
>NP_003801
          Length = 281

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   IFISVTQE 9
           IF+SVT E
Sbjct: 256 IFVSVTNE 263
>NP_620168
          Length = 1300

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YIFISVTQE 9
           Y+F+S TQ+
Sbjct: 608 YVFLSRTQD 616
>NP_056312
          Length = 555

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   YIFISVTQ 8
           +IFIS+TQ
Sbjct: 377 WIFISLTQ 384
>NP_001273416
          Length = 494

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   YIFISVTQ 8
           +IFIS+TQ
Sbjct: 316 WIFISLTQ 323
>NP_001171473
          Length = 1263

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YIFISVTQE 9
           Y+F+S TQ+
Sbjct: 561 YVFLSRTQD 569
>XP_006722720
          Length = 820

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YIFISVTQE 9
           Y+F+S TQ+
Sbjct: 608 YVFLSRTQD 616
>XP_006722719
          Length = 1224

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YIFISVTQE 9
           Y+F+S TQ+
Sbjct: 522 YVFLSRTQD 530
>XP_006722718
          Length = 1310

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YIFISVTQE 9
           Y+F+S TQ+
Sbjct: 608 YVFLSRTQD 616
>XP_006722717
          Length = 1310

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   YIFISVTQE 9
           Y+F+S TQ+
Sbjct: 608 YVFLSRTQD 616
>XP_006720531
          Length = 415

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   YIFISVTQ 8
           +IFIS+TQ
Sbjct: 237 WIFISLTQ 244
>XP_005254344
          Length = 556

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1   YIFISVTQ 8
           +IFIS+TQ
Sbjct: 378 WIFISLTQ 385
>NP_001005499
          Length = 312

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YIFISVTQ 8
           YIF+ VT+
Sbjct: 102 YIFLGVTE 109
>NP_001230396
          Length = 526

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YIFISVTQE 9
           YIF+  TQ+
Sbjct: 264 YIFVDPTQQ 272
>NP_061834
          Length = 661

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YIFISVTQE 9
           YIF+  TQ+
Sbjct: 302 YIFVDPTQQ 310
>NP_689646
          Length = 612

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YIFISVTQE 9
           YIFI  T+E
Sbjct: 296 YIFIHKTEE 304
>NP_940841
          Length = 612

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YIFISVTQE 9
           YIFI  T+E
Sbjct: 296 YIFIHKTEE 304
>NP_001001895
          Length = 623

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YIFISVTQE 9
           YIF+  TQ+
Sbjct: 264 YIFVDPTQQ 272
>XP_006724076
          Length = 642

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YIFISVTQE 9
           YIF+  TQ+
Sbjct: 283 YIFVDPTQQ 291
>XP_006718830
          Length = 612

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YIFISVTQE 9
           YIFI  T+E
Sbjct: 296 YIFIHKTEE 304
>NP_001138432
          Length = 544

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  YIFISVTQE 9
          Y+F +VTQ+
Sbjct: 86 YVFNAVTQD 94
>NP_001035521
          Length = 106

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  YIFISVTQE 9
          Y F S++QE
Sbjct: 74 YTFYSISQE 82
>NP_001004730
          Length = 310

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  YIFISVTQE 9
          +IF+ +TQ+
Sbjct: 12 FIFMGITQD 20
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.141    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 343,394
Number of extensions: 571
Number of successful extensions: 26
Number of sequences better than 10000.0: 26
Number of HSP's gapped: 26
Number of HSP's successfully gapped: 26
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)

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