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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004963                                                              20     299
XP_005251512                                                           20     299
NP_004275                                                              17    3301
NP_001243265                                                           17    3301
NP_078844                                                              17    3301
NP_001243267                                                           17    3301
XP_006711702                                                           17    3301
XP_006711701                                                           17    3301
XP_006711700                                                           17    3301
XP_006711699                                                           17    3301
NP_006059                                                              17    4312
XP_005262694                                                           17    4312
XP_005262693                                                           17    4312
NP_958934                                                              15    9605
NP_005536                                                              15    9605

>NP_004963
          Length = 1132

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  YHNMSALMS 9
          YHNM ALMS
Sbjct: 81 YHNMFALMS 89



>XP_005251512
          Length = 1132

 Score = 20.4 bits (41), Expect =   299
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  YHNMSALMS 9
          YHNM ALMS
Sbjct: 81 YHNMFALMS 89



>NP_004275
          Length = 897

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YHNMSAL 7
           YH MSAL
Sbjct: 279 YHGMSAL 285



>NP_001243265
          Length = 797

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YHNMSAL 7
           YH MSAL
Sbjct: 179 YHGMSAL 185



>NP_078844
          Length = 784

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YHNMSAL 7
           YH MSAL
Sbjct: 166 YHGMSAL 172



>NP_001243267
          Length = 693

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1  YHNMSAL 7
          YH MSAL
Sbjct: 75 YHGMSAL 81



>XP_006711702
          Length = 784

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YHNMSAL 7
           YH MSAL
Sbjct: 166 YHGMSAL 172



>XP_006711701
          Length = 792

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YHNMSAL 7
           YH MSAL
Sbjct: 279 YHGMSAL 285



>XP_006711700
          Length = 803

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YHNMSAL 7
           YH MSAL
Sbjct: 279 YHGMSAL 285



>XP_006711699
          Length = 825

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YHNMSAL 7
           YH MSAL
Sbjct: 207 YHGMSAL 213



>NP_006059
          Length = 796

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YHNMSALMS 9
           YH + ALM+
Sbjct: 748 YHKLKALMT 756



>XP_005262694
          Length = 796

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YHNMSALMS 9
           YH + ALM+
Sbjct: 748 YHKLKALMT 756



>XP_005262693
          Length = 796

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   YHNMSALMS 9
           YH + ALM+
Sbjct: 748 YHKLKALMT 756



>NP_958934
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HNMSAL 7
           HN+SAL
Sbjct: 119 HNLSAL 124



>NP_005536
          Length = 428

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   HNMSAL 7
           HN+SAL
Sbjct: 119 HNLSAL 124



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.321    0.116    0.329 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 332,241
Number of extensions: 217
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)