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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_003290                                                              20     390
NP_443199                                                              18    1482
XP_005248167                                                           17    3301
NP_001121636                                                           17    4312
NP_000323                                                              17    4312
NP_036245                                                              16    5631
XP_006711324                                                           16    5631
XP_006711323                                                           16    5631
XP_005245116                                                           16    5631
NP_940905                                                              16    7355
NP_001107604                                                           16    7355
XP_005246845                                                           16    7355
XP_006712765                                                           16    7355
XP_006712764                                                           16    7355
XP_005246844                                                           16    7355
XP_006712763                                                           16    7355
XP_006712762                                                           16    7355
XP_006712761                                                           16    7355
XP_006711360                                                           16    7355
XP_006711359                                                           16    7355
XP_006711357                                                           16    7355
NP_001028252                                                           16    7355
NP_055441                                                              16    7355
NP_061918                                                              15    9605
NP_001129663                                                           15    9605
NP_001171894                                                           15    9605
NP_056308                                                              15    9605
NP_003117                                                              15    9605
XP_005245596                                                           15    9605

>NP_003290
          Length = 803

 Score = 20.0 bits (40), Expect =   390
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   YFMAGSIRK 9
           YFMAGS RK
Sbjct: 539 YFMAGSSRK 547



>NP_443199
          Length = 378

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YFMAGSIRK 9
           YF  GS+RK
Sbjct: 314 YFFVGSLRK 322



>XP_005248167
          Length = 592

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   YFMAGSIR 8
           YF+ GSIR
Sbjct: 371 YFVKGSIR 378



>NP_001121636
          Length = 815

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YFMAGSI 7
           YFM GSI
Sbjct: 573 YFMKGSI 579



>NP_000323
          Length = 815

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   YFMAGSI 7
           YFM GSI
Sbjct: 573 YFMKGSI 579



>NP_036245
          Length = 337

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YFMAGSIRK 9
           YF+A  IRK
Sbjct: 309 YFIARKIRK 317



>XP_006711324
          Length = 281

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YFMAGSIRK 9
           YF+A  IRK
Sbjct: 253 YFIARKIRK 261



>XP_006711323
          Length = 306

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YFMAGSIRK 9
           YF+A  IRK
Sbjct: 278 YFIARKIRK 286



>XP_005245116
          Length = 336

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   YFMAGSIRK 9
           YF+A  IRK
Sbjct: 308 YFIARKIRK 316



>NP_940905
          Length = 1135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YFMAGSI 7
           Y+M GSI
Sbjct: 775 YYMVGSI 781



>NP_001107604
          Length = 2694

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YFMAGSIRK 9
            YF  GSI K
Sbjct: 1915 YFYDGSIEK 1923



>XP_005246845
          Length = 1624

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1   YFMAGSIRK 9
           YF  GSI K
Sbjct: 832 YFYDGSIEK 840



>XP_006712765
          Length = 2679

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YFMAGSIRK 9
            YF  GSI K
Sbjct: 1887 YFYDGSIEK 1895



>XP_006712764
          Length = 2697

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YFMAGSIRK 9
            YF  GSI K
Sbjct: 1944 YFYDGSIEK 1952



>XP_005246844
          Length = 2707

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YFMAGSIRK 9
            YF  GSI K
Sbjct: 1915 YFYDGSIEK 1923



>XP_006712763
          Length = 2716

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YFMAGSIRK 9
            YF  GSI K
Sbjct: 1944 YFYDGSIEK 1952



>XP_006712762
          Length = 2723

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YFMAGSIRK 9
            YF  GSI K
Sbjct: 1944 YFYDGSIEK 1952



>XP_006712761
          Length = 2736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 6/9 (66%)

Query: 1    YFMAGSIRK 9
            YF  GSI K
Sbjct: 1944 YFYDGSIEK 1952



>XP_006711360
          Length = 699

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YFMAGSI 7
           Y+M GSI
Sbjct: 332 YYMVGSI 338



>XP_006711359
          Length = 752

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YFMAGSI 7
           Y+M GSI
Sbjct: 385 YYMVGSI 391



>XP_006711357
          Length = 1142

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   YFMAGSI 7
           Y+M GSI
Sbjct: 775 YYMVGSI 781



>NP_001028252
          Length = 351

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2  FMAGSI 7
          FMAGSI
Sbjct: 49 FMAGSI 54



>NP_055441
          Length = 306

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2 FMAGSI 7
         FMAGSI
Sbjct: 4 FMAGSI 9



>NP_061918
          Length = 913

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   YFMAGSI 7
           YF++GS+
Sbjct: 662 YFLSGSL 668



>NP_001129663
          Length = 851

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MAGSIRK 9
           MAG++RK
Sbjct: 192 MAGALRK 198



>NP_001171894
          Length = 905

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   YFMAGSI 7
           YF++GS+
Sbjct: 662 YFLSGSL 668



>NP_056308
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   MAGSIRK 9
           MAG++RK
Sbjct: 192 MAGALRK 198



>NP_003117
          Length = 2419

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    YFMAGSIRK 9
            YF+ GS+ K
Sbjct: 2179 YFLDGSLLK 2187



>XP_005245596
          Length = 362

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   YFMAGSIR 8
           YF+A S+R
Sbjct: 330 YFLAWSLR 337



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.336    0.144    0.422 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 418,200
Number of extensions: 631
Number of successful extensions: 29
Number of sequences better than 10000.0: 29
Number of HSP's gapped: 29
Number of HSP's successfully gapped: 29
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.4 bits)
S2: 28 (15.4 bits)