Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001243398                                                           18    1135
NP_036462                                                              18    1135
NP_001243397                                                           18    1135
XP_005269721                                                           18    1135
NP_036418                                                              17    2528
NP_001135891                                                           17    2528
NP_001135897                                                           17    2528
NP_001135893                                                           17    2528
NP_001135898                                                           17    2528
NP_001135895                                                           17    2528
NP_001135892                                                           17    2528
NP_001135894                                                           17    2528
NP_001135904                                                           17    2528
NP_001135903                                                           17    2528
NP_001135902                                                           17    2528
NP_001135901                                                           17    2528
NP_001135899                                                           17    2528
NP_001135890                                                           17    2528
NP_001135896                                                           17    2528
NP_001135900                                                           17    2528
NP_009003                                                              16    5631
NP_001269485                                                           16    5631
NP_001269486                                                           16    5631
NP_001269487                                                           16    5631
XP_005247137                                                           16    5631
NP_060499                                                              16    7355
XP_006719430                                                           15    9605
XP_006719429                                                           15    9605
XP_006719428                                                           15    9605
XP_005253933                                                           15    9605
NP_001084                                                              15    9605
YP_003024038                                                           15    9605
>NP_001243398
          Length = 1781

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    YANSASLFT 9
            YANSASL T
Sbjct: 1551 YANSASLST 1559
>NP_036462
          Length = 2073

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    YANSASLFT 9
            YANSASL T
Sbjct: 1843 YANSASLST 1851
>NP_001243397
          Length = 1890

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    YANSASLFT 9
            YANSASL T
Sbjct: 1660 YANSASLST 1668
>XP_005269721
          Length = 2073

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    YANSASLFT 9
            YANSASL T
Sbjct: 1843 YANSASLST 1851
>NP_036418
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135891
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135897
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135893
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135898
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135895
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135892
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135894
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135904
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135903
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135902
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135901
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135899
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135890
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135896
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_001135900
          Length = 288

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   NSASLFT 9
           NSASLFT
Sbjct: 266 NSASLFT 272
>NP_009003
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  YANSASLF 8
          YAN  SLF
Sbjct: 88 YANRTSLF 95
>NP_001269485
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  YANSASLF 8
          YAN  SLF
Sbjct: 88 YANRTSLF 95
>NP_001269486
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  YANSASLF 8
          YAN  SLF
Sbjct: 88 YANRTSLF 95
>NP_001269487
          Length = 397

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  YANSASLF 8
          YAN  SLF
Sbjct: 88 YANRTSLF 95
>XP_005247137
          Length = 414

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  YANSASLF 8
          YAN  SLF
Sbjct: 88 YANRTSLF 95
>NP_060499
          Length = 272

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  YANSASLF 8
          Y NS SLF
Sbjct: 10 YVNSHSLF 17
>XP_006719430
          Length = 2256

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   YANSASLF 8
           Y N+ SLF
Sbjct: 308 YGNAVSLF 315
>XP_006719429
          Length = 2395

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   YANSASLF 8
           Y N+ SLF
Sbjct: 510 YGNAVSLF 517
>XP_006719428
          Length = 2458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   YANSASLF 8
           Y N+ SLF
Sbjct: 510 YGNAVSLF 517
>XP_005253933
          Length = 2458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   YANSASLF 8
           Y N+ SLF
Sbjct: 510 YGNAVSLF 517
>NP_001084
          Length = 2458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1   YANSASLF 8
           Y N+ SLF
Sbjct: 510 YGNAVSLF 517
>YP_003024038
          Length = 380

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  YANSASLF 8
          +AN AS+F
Sbjct: 83 HANGASMF 90
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.116    0.305 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 319,867
Number of extensions: 319
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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