Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              22      79
NP_001075109                                                           22      79
NP_694999                                                              18    1482
NP_001277060                                                           18    1482
NP_001028733                                                           18    1482
NP_065940                                                              18    1482
XP_005258073                                                           18    1482
XP_005258072                                                           18    1482
NP_004668                                                              17    3301
NP_001002906                                                           17    3301
NP_689588                                                              17    3301
NP_821174                                                              17    4312
NP_060340                                                              17    4312
NP_116570                                                              16    5631
NP_116572                                                              16    5631
NP_116571                                                              16    5631
NP_116569                                                              16    5631
NP_001135797                                                           16    5631
NP_001135796                                                           16    5631
NP_003044                                                              16    5631
NP_001129163                                                           16    5631
NP_653267                                                              16    5631
NP_849143                                                              16    5631
XP_005266247                                                           16    5631
XP_005266245                                                           16    5631
XP_006716082                                                           16    5631
XP_006716081                                                           16    5631
XP_005250456                                                           16    5631
XP_006716080                                                           16    5631
XP_006716079                                                           16    5631
NP_001001954                                                           16    5631
NP_003431                                                              16    5631
NP_000104                                                              16    7355
NP_056099                                                              16    7355
NP_057263                                                              16    7355
NP_001129430                                                           16    7355
NP_001129428                                                           16    7355
NP_001129427                                                           16    7355
NP_003297                                                              16    7355
NP_060684                                                              16    7355
XP_005272048                                                           16    7355
XP_006714680                                                           16    7355
XP_006710565                                                           16    7355
XP_006710564                                                           16    7355
XP_005270743                                                           16    7355
NP_001129429                                                           16    7355
NP_001035805                                                           16    7355
NP_005752                                                              15    9605
NP_000287                                                              15    9605
NP_001009944                                                           15    9605
NP_004548                                                              15    9605
NP_006019                                                              15    9605
NP_001099001                                                           15    9605
NP_065870                                                              15    9605
NP_001156001                                                           15    9605
NP_036603                                                              15    9605
NP_057323                                                              15    9605
XP_006726693                                                           15    9605
XP_005256732                                                           15    9605
XP_006720958                                                           15    9605
XP_006720957                                                           15    9605
XP_006720956                                                           15    9605
XP_006720955                                                           15    9605
XP_006720954                                                           15    9605
XP_006720953                                                           15    9605
XP_006720952                                                           15    9605
XP_006719249                                                           15    9605
XP_005248808                                                           15    9605
XP_005248807                                                           15    9605
XP_005248806                                                           15    9605
XP_005248805                                                           15    9605
XP_005248804                                                           15    9605
XP_005248803                                                           15    9605
XP_005248802                                                           15    9605
XP_005248801                                                           15    9605
XP_005248800                                                           15    9605
NP_055053                                                              15    9605
>NP_008835
          Length = 4128

 Score = 22.3 bits (46), Expect =    79
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    WHCSLVALR 9
            WHCSL ALR
Sbjct: 1829 WHCSLDALR 1837
>NP_001075109
          Length = 4097

 Score = 22.3 bits (46), Expect =    79
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    WHCSLVALR 9
            WHCSL ALR
Sbjct: 1829 WHCSLDALR 1837
>NP_694999
          Length = 686

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   WHCSLVAL 8
           W C LVAL
Sbjct: 479 WDCGLVAL 486
>NP_001277060
          Length = 849

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   WHCSLVALR 9
           W CS+VAL+
Sbjct: 432 WLCSVVALQ 440
>NP_001028733
          Length = 1004

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   WHCSLVALR 9
           W CS+VAL+
Sbjct: 432 WLCSVVALQ 440
>NP_065940
          Length = 704

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   WHCSLVALR 9
           W CS+VAL+
Sbjct: 432 WLCSVVALQ 440
>XP_005258073
          Length = 787

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   WHCSLVALR 9
           W CS+VAL+
Sbjct: 215 WLCSVVALQ 223
>XP_005258072
          Length = 914

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   WHCSLVALR 9
           W CS+VAL+
Sbjct: 342 WLCSVVALQ 350
>NP_004668
          Length = 117

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  HCSLVALR 9
          HC+++A+R
Sbjct: 22 HCNILAIR 29
>NP_001002906
          Length = 117

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2  HCSLVALR 9
          HC+++A+R
Sbjct: 22 HCNILAIR 29
>NP_689588
          Length = 777

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HCSLVAL 8
           HC LVAL
Sbjct: 581 HCRLVAL 587
>NP_821174
          Length = 1023

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  WHCSLVAL 8
          W C LVAL
Sbjct: 20 WLCLLVAL 27
>NP_060340
          Length = 1024

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1  WHCSLVAL 8
          W C LVAL
Sbjct: 20 WLCLLVAL 27
>NP_116570
          Length = 833

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HCSLVALR 9
           +CSL ALR
Sbjct: 786 YCSLPALR 793
>NP_116572
          Length = 831

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HCSLVALR 9
           +CSL ALR
Sbjct: 784 YCSLPALR 791
>NP_116571
          Length = 850

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HCSLVALR 9
           +CSL ALR
Sbjct: 803 YCSLPALR 810
>NP_116569
          Length = 852

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HCSLVALR 9
           +CSL ALR
Sbjct: 805 YCSLPALR 812
>NP_001135797
          Length = 472

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   WHCSLVAL 8
           W CSL+ +
Sbjct: 366 WLCSLIGM 373
>NP_001135796
          Length = 493

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   WHCSLVAL 8
           W CSL+ +
Sbjct: 334 WLCSLIGM 341
>NP_003044
          Length = 525

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   WHCSLVAL 8
           W CSL+ +
Sbjct: 366 WLCSLIGM 373
>NP_001129163
          Length = 525

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 6/8 (75%)

Query: 1   WHCSLVAL 8
           W CSL+ +
Sbjct: 366 WLCSLIGM 373
>NP_653267
          Length = 4753

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1    WHCSLVA 7
            W C LVA
Sbjct: 1508 WQCVLVA 1514
>NP_849143
          Length = 415

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   WHCSLV 6
           W CSL+
Sbjct: 271 WDCSLI 276
>XP_005266247
          Length = 354

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   WHCSLV 6
           WH SL+
Sbjct: 265 WHASLI 270
>XP_005266245
          Length = 423

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   WHCSLV 6
           WH SL+
Sbjct: 334 WHASLI 339
>XP_006716082
          Length = 711

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HCSLVALR 9
           +CSL ALR
Sbjct: 664 YCSLPALR 671
>XP_006716081
          Length = 738

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HCSLVALR 9
           +CSL ALR
Sbjct: 691 YCSLPALR 698
>XP_005250456
          Length = 878

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HCSLVALR 9
           +CSL ALR
Sbjct: 831 YCSLPALR 838
>XP_006716080
          Length = 886

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HCSLVALR 9
           +CSL ALR
Sbjct: 839 YCSLPALR 846
>XP_006716079
          Length = 904

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   HCSLVALR 9
           +CSL ALR
Sbjct: 857 YCSLPALR 864
>NP_001001954
          Length = 324

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  WHCSLVAL 8
          W+ SL+AL
Sbjct: 42 WNLSLIAL 49
>NP_003431
          Length = 457

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   WHCSLV 6
           WH SL+
Sbjct: 368 WHASLI 373
>NP_000104
          Length = 332

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 1   WHCSLV 6
           WH SL+
Sbjct: 253 WHSSLI 258
>NP_056099
          Length = 3375

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    WHCSLVALR 9
            + CSL ALR
Sbjct: 1185 YSCSLEALR 1193
>NP_057263
          Length = 977

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WHCSLVA 7
           WH +LVA
Sbjct: 471 WHPTLVA 477
>NP_001129430
          Length = 804

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WHCSLVA 7
           WH +LVA
Sbjct: 298 WHPTLVA 304
>NP_001129428
          Length = 828

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WHCSLVA 7
           WH +LVA
Sbjct: 471 WHPTLVA 477
>NP_001129427
          Length = 893

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WHCSLVA 7
           WH +LVA
Sbjct: 471 WHPTLVA 477
>NP_003297
          Length = 982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WHCSLVA 7
           WH +LVA
Sbjct: 471 WHPTLVA 477
>NP_060684
          Length = 524

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2  HCSLV 6
          HCSLV
Sbjct: 21 HCSLV 25
>XP_005272048
          Length = 851

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   CSLVAL 8
           CSLVAL
Sbjct: 697 CSLVAL 702
>XP_006714680
          Length = 1002

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3   CSLVAL 8
           CSLVAL
Sbjct: 848 CSLVAL 853
>XP_006710565
          Length = 3140

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   WHCSLVALR 9
           + CSL ALR
Sbjct: 950 YSCSLEALR 958
>XP_006710564
          Length = 3432

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    WHCSLVALR 9
            + CSL ALR
Sbjct: 1242 YSCSLEALR 1250
>XP_005270743
          Length = 3453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1    WHCSLVALR 9
            + CSL ALR
Sbjct: 1263 YSCSLEALR 1271
>NP_001129429
          Length = 836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WHCSLVA 7
           WH +LVA
Sbjct: 471 WHPTLVA 477
>NP_001035805
          Length = 471

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 3   CSLVALR 9
           C LVALR
Sbjct: 138 CMLVALR 144
>NP_005752
          Length = 1568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2   HCSLV 6
           HCSL+
Sbjct: 755 HCSLI 759
>NP_000287
          Length = 4302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   WHCSLVALR 9
           W C   ALR
Sbjct: 762 WACPACALR 770
>NP_001009944
          Length = 4303

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   WHCSLVALR 9
           W C   ALR
Sbjct: 762 WACPACALR 770
>NP_004548
          Length = 2003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 5/8 (62%)

Query: 1    WHCSLVAL 8
            W C  VAL
Sbjct: 1949 WPCDWVAL 1956
>NP_006019
          Length = 441

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 5/7 (71%)

Query: 1  WHCSLVA 7
          W C LVA
Sbjct: 10 WACILVA 16
>NP_001099001
          Length = 1319

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HCSLVAL 8
           H SLVAL
Sbjct: 169 HASLVAL 175
>NP_065870
          Length = 1302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HCSLVAL 8
           H SLVAL
Sbjct: 126 HASLVAL 132
>NP_001156001
          Length = 1515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HCSLVAL 8
           H SLVAL
Sbjct: 169 HASLVAL 175
>NP_036603
          Length = 973

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   WHCSLVA 7
           WH +L+A
Sbjct: 472 WHPTLIA 478
>NP_057323
          Length = 3530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HCSLVALR 9
            +C LVAL+
Sbjct: 1845 YCCLVALK 1852
>XP_006726693
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   WHCSLVALR 9
           W C   ALR
Sbjct: 159 WACPACALR 167
>XP_005256732
          Length = 3531

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    HCSLVALR 9
            +C LVAL+
Sbjct: 1846 YCCLVALK 1853
>XP_006720958
          Length = 2675

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   WHCSLVALR 9
           W C   ALR
Sbjct: 799 WACPACALR 807
>XP_006720957
          Length = 2688

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   WHCSLVALR 9
           W C   ALR
Sbjct: 799 WACPACALR 807
>XP_006720956
          Length = 3895

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   WHCSLVALR 9
           W C   ALR
Sbjct: 354 WACPACALR 362
>XP_006720955
          Length = 4279

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   WHCSLVALR 9
           W C   ALR
Sbjct: 738 WACPACALR 746
>XP_006720954
          Length = 4334

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   WHCSLVALR 9
           W C   ALR
Sbjct: 799 WACPACALR 807
>XP_006720953
          Length = 4339

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   WHCSLVALR 9
           W C   ALR
Sbjct: 799 WACPACALR 807
>XP_006720952
          Length = 4340

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 5/9 (55%)

Query: 1   WHCSLVALR 9
           W C   ALR
Sbjct: 799 WACPACALR 807
>XP_006719249
          Length = 1555

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/5 (80%), Positives = 5/5 (100%)

Query: 2   HCSLV 6
           HCSL+
Sbjct: 755 HCSLI 759
>XP_005248808
          Length = 1121

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HCSLVAL 8
           H SLVAL
Sbjct: 169 HASLVAL 175
>XP_005248807
          Length = 1182

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  HCSLVAL 8
          H SLVAL
Sbjct: 6  HASLVAL 12
>XP_005248806
          Length = 1317

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HCSLVAL 8
           H SLVAL
Sbjct: 169 HASLVAL 175
>XP_005248805
          Length = 1345

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HCSLVAL 8
           H SLVAL
Sbjct: 169 HASLVAL 175
>XP_005248804
          Length = 1378

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  HCSLVAL 8
          H SLVAL
Sbjct: 6  HASLVAL 12
>XP_005248803
          Length = 1455

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2  HCSLVAL 8
          H SLVAL
Sbjct: 83 HASLVAL 89
>XP_005248802
          Length = 1515

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HCSLVAL 8
           H SLVAL
Sbjct: 169 HASLVAL 175
>XP_005248801
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HCSLVAL 8
           H SLVAL
Sbjct: 169 HASLVAL 175
>XP_005248800
          Length = 1541

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   HCSLVAL 8
           H SLVAL
Sbjct: 169 HASLVAL 175
>NP_055053
          Length = 762

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 5/7 (71%)

Query: 1   WHCSLVA 7
           W C L+A
Sbjct: 374 WKCLLIA 380
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.338    0.136    0.603 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 551,644
Number of extensions: 554
Number of successful extensions: 77
Number of sequences better than 10000.0: 77
Number of HSP's gapped: 77
Number of HSP's successfully gapped: 77
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.5 bits)
S2: 28 (15.4 bits)

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