Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_073713                                                              19     869
NP_001092882                                                           17    2528
NP_006757                                                              17    2528
NP_001092883                                                           17    2528
XP_005273706                                                           17    2528
NP_859529                                                              17    3301
NP_001182684                                                           17    3301
NP_872577                                                              17    4312
NP_000186                                                              17    4312
NP_003569                                                              17    4312
NP_001186673                                                           17    4312
XP_006717881                                                           17    4312
XP_005269815                                                           17    4312
XP_005269813                                                           17    4312
XP_005269814                                                           17    4312
XP_005269812                                                           17    4312
NP_008864                                                              16    5631
NP_001128493                                                           16    5631
NP_001128495                                                           16    5631
NP_001128494                                                           16    5631
NP_115589                                                              16    5631
XP_006712179                                                           16    5631
XP_005264655                                                           16    5631
XP_005264654                                                           16    5631
XP_005264653                                                           16    5631
NP_001018048                                                           16    7355
NP_056001                                                              16    7355
NP_001018047                                                           16    7355
NP_150648                                                              16    7355
NP_859049                                                              16    7355
NP_001269781                                                           16    7355
NP_659449                                                              16    7355
NP_001121371                                                           16    7355
NP_001269092                                                           16    7355
NP_001121370                                                           16    7355
NP_000075                                                              16    7355
NP_078930                                                              16    7355
NP_001269782                                                           16    7355
NP_001269783                                                           16    7355
XP_005252445                                                           16    7355
XP_006717455                                                           16    7355
XP_005263550                                                           16    7355
NP_598004                                                              15    9605
NP_068570                                                              15    9605
NP_851564                                                              15    9605
NP_851565                                                              15    9605
NP_001106995                                                           15    9605
NP_001399                                                              15    9605
XP_005247218                                                           15    9605
XP_005247217                                                           15    9605
XP_005247216                                                           15    9605
XP_005247149                                                           15    9605
XP_006710468                                                           15    9605
XP_005270637                                                           15    9605
>NP_073713
          Length = 985

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   APIIVSTM 9
           APIIVSTM
Sbjct: 547 APIIVSTM 554
>NP_001092882
          Length = 2004

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W P+IVS
Sbjct: 776 WTPVIVS 782
>NP_006757
          Length = 2004

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W P+IVS
Sbjct: 776 WTPVIVS 782
>NP_001092883
          Length = 2004

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W P+IVS
Sbjct: 776 WTPVIVS 782
>XP_005273706
          Length = 1997

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W P+IVS
Sbjct: 769 WTPVIVS 775
>NP_859529
          Length = 377

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   WAPIIVSTM 9
           W+PII++ +
Sbjct: 293 WSPIIITIL 301
>NP_001182684
          Length = 361

 Score = 16.9 bits (32), Expect =  3301
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   WAPIIVSTM 9
           W+PII++ +
Sbjct: 277 WSPIIITIL 285
>NP_872577
          Length = 324

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  APIIVSTM 9
          AP+I+S+M
Sbjct: 52 APVIISSM 59
>NP_000186
          Length = 700

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  APIIVSTM 9
          AP+I+S+M
Sbjct: 52 APVIISSM 59
>NP_003569
          Length = 522

 Score = 16.5 bits (31), Expect =  4312
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   WAPIIVSTM 9
           W P+ +ST+
Sbjct: 170 WVPMFLSTL 178
>NP_001186673
          Length = 208

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  WAPIIVSTM 9
          WA  ++STM
Sbjct: 71 WAAEVISTM 79
>XP_006717881
          Length = 613

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  APIIVSTM 9
          AP+I+S+M
Sbjct: 52 APVIISSM 59
>XP_005269815
          Length = 667

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  APIIVSTM 9
          AP+I+S+M
Sbjct: 52 APVIISSM 59
>XP_005269813
          Length = 700

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  APIIVSTM 9
          AP+I+S+M
Sbjct: 52 APVIISSM 59
>XP_005269814
          Length = 700

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  APIIVSTM 9
          AP+I+S+M
Sbjct: 52 APVIISSM 59
>XP_005269812
          Length = 700

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2  APIIVSTM 9
          AP+I+S+M
Sbjct: 52 APVIISSM 59
>NP_008864
          Length = 718

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   WAPIIV 6
           W PIIV
Sbjct: 425 WVPIIV 430
>NP_001128493
          Length = 381

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WAPIIVST 8
          W P +VST
Sbjct: 63 WEPRVVST 70
>NP_001128495
          Length = 381

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WAPIIVST 8
          W P +VST
Sbjct: 63 WEPRVVST 70
>NP_001128494
          Length = 381

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WAPIIVST 8
          W P +VST
Sbjct: 63 WEPRVVST 70
>NP_115589
          Length = 391

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WAPIIVST 8
          W P +VST
Sbjct: 63 WEPRVVST 70
>XP_006712179
          Length = 251

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WAPIIVST 8
          W P +VST
Sbjct: 63 WEPRVVST 70
>XP_005264655
          Length = 391

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WAPIIVST 8
          W P +VST
Sbjct: 63 WEPRVVST 70
>XP_005264654
          Length = 391

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WAPIIVST 8
          W P +VST
Sbjct: 63 WEPRVVST 70
>XP_005264653
          Length = 391

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 1  WAPIIVST 8
          W P +VST
Sbjct: 63 WEPRVVST 70
>NP_001018048
          Length = 3069

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    APIIVSTM 9
            +P+I++TM
Sbjct: 1654 SPVIINTM 1661
>NP_056001
          Length = 3095

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    APIIVSTM 9
            +P+I++TM
Sbjct: 1654 SPVIINTM 1661
>NP_001018047
          Length = 3135

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    APIIVSTM 9
            +P+I++TM
Sbjct: 1615 SPVIINTM 1622
>NP_150648
          Length = 3174

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2    APIIVSTM 9
            +P+I++TM
Sbjct: 1654 SPVIINTM 1661
>NP_859049
          Length = 287

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W+P I+S
Sbjct: 281 WSPAIIS 287
>NP_001269781
          Length = 316

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W+P I+S
Sbjct: 310 WSPAIIS 316
>NP_659449
          Length = 341

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W+P I+S
Sbjct: 335 WSPAIIS 341
>NP_001121371
          Length = 816

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   WAPIIVST 8
           W+ +I+ST
Sbjct: 192 WSQLIIST 199
>NP_001269092
          Length = 766

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   WAPIIVST 8
           W+ +I+ST
Sbjct: 142 WSQLIIST 149
>NP_001121370
          Length = 816

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   WAPIIVST 8
           W+ +I+ST
Sbjct: 192 WSQLIIST 199
>NP_000075
          Length = 746

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   WAPIIVST 8
           W+ +I+ST
Sbjct: 122 WSQLIIST 129
>NP_078930
          Length = 702

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   APIIVSTM 9
           +P+IV+TM
Sbjct: 401 SPLIVNTM 408
>NP_001269782
          Length = 287

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W+P I+S
Sbjct: 281 WSPAIIS 287
>NP_001269783
          Length = 208

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W+P I+S
Sbjct: 202 WSPAIIS 208
>XP_005252445
          Length = 294

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W+P I+S
Sbjct: 288 WSPAIIS 294
>XP_006717455
          Length = 317

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/7 (57%), Positives = 6/7 (85%)

Query: 1   WAPIIVS 7
           W+P I+S
Sbjct: 311 WSPAIIS 317
>XP_005263550
          Length = 415

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   APIIVSTM 9
           +P+IV+TM
Sbjct: 114 SPLIVNTM 121
>NP_598004
          Length = 545

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   WAPIIVSTM 9
           WA + +ST+
Sbjct: 450 WAAVSISTV 458
>NP_068570
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   WAPIIVSTM 9
           W+P + ST+
Sbjct: 306 WSPEVPSTL 314
>NP_851564
          Length = 538

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   WAPIIVSTM 9
           W+P + ST+
Sbjct: 306 WSPEVPSTL 314
>NP_851565
          Length = 560

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 7/9 (77%)

Query: 1   WAPIIVSTM 9
           W+P + ST+
Sbjct: 328 WSPEVPSTL 336
>NP_001106995
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   WAPIIVSTM 9
           WA +IV+++
Sbjct: 102 WADVIVNSV 110
>NP_001399
          Length = 2923

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   APIIVST 8
           API VST
Sbjct: 502 APIFVST 508
>XP_005247218
          Length = 483

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   WAPIIVSTM 9
           WA +IV+++
Sbjct: 102 WADVIVNSV 110
>XP_005247217
          Length = 602

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   WAPIIVSTM 9
           WA +IV+++
Sbjct: 102 WADVIVNSV 110
>XP_005247216
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   WAPIIVSTM 9
           WA +IV+++
Sbjct: 102 WADVIVNSV 110
>XP_005247149
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1  WAPIIVSTM 9
          W P  VST+
Sbjct: 10 WDPTTVSTV 18
>XP_006710468
          Length = 2916

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   APIIVST 8
           API VST
Sbjct: 502 APIFVST 508
>XP_005270637
          Length = 2921

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   APIIVST 8
           API VST
Sbjct: 502 APIFVST 508
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.328    0.131    0.481 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 406,698
Number of extensions: 524
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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