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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              18    1482
NP_653080                                                              18    1482
XP_005267292                                                           18    1482
XP_005267291                                                           18    1482
XP_005267290                                                           18    1482
NP_003290                                                              15    9605
NP_056234                                                              15    9605
XP_005274542                                                           15    9605
NP_001019765                                                           15    9605
NP_001131032                                                           15    9605

>NP_005091
          Length = 1782

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVSEKLTAS 9
           V SEKLTAS
Sbjct: 345 VASEKLTAS 353



>NP_653080
          Length = 1684

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVSEKLTAS 9
           V SEKLTAS
Sbjct: 247 VASEKLTAS 255



>XP_005267292
          Length = 1677

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVSEKLTAS 9
           V SEKLTAS
Sbjct: 240 VASEKLTAS 248



>XP_005267291
          Length = 1684

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVSEKLTAS 9
           V SEKLTAS
Sbjct: 247 VASEKLTAS 255



>XP_005267290
          Length = 1782

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVSEKLTAS 9
           V SEKLTAS
Sbjct: 345 VASEKLTAS 353



>NP_003290
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VVSEKLTAS 9
           VVS++LT S
Sbjct: 635 VVSQRLTES 643



>NP_056234
          Length = 2828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VSEKLTA 8
            +SEK+TA
Sbjct: 2548 ISEKITA 2554



>XP_005274542
          Length = 2853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2    VSEKLTA 8
            +SEK+TA
Sbjct: 2573 ISEKITA 2579



>NP_001019765
          Length = 145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VSEKLTAS 9
          VSE+LTA+
Sbjct: 68 VSEELTAA 75



>NP_001131032
          Length = 566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  VSEKLTAS 9
          VSE+LTA+
Sbjct: 24 VSEELTAA 31



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.293    0.106    0.224 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 345,330
Number of extensions: 172
Number of successful extensions: 10
Number of sequences better than 10000.0: 10
Number of HSP's gapped: 10
Number of HSP's successfully gapped: 10
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)