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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_722576                                                              18    1935
XP_005262424                                                           18    1935
NP_001152768                                                           16    5631
NP_009181                                                              16    5631
NP_004215                                                              16    5631
XP_005274829                                                           16    5631
XP_006724639                                                           16    5631
XP_006724638                                                           16    5631
XP_005262036                                                           16    5631
XP_005262035                                                           16    5631
XP_005262034                                                           16    5631
NP_001489                                                              15    9605
NP_714921                                                              15    9605
NP_001184044                                                           15    9605
XP_005271739                                                           15    9605
NP_003469                                                              15    9605

>NP_722576
          Length = 3080

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    VVSASISD 8
            VVSASISD
Sbjct: 2649 VVSASISD 2656



>XP_005262424
          Length = 2875

 Score = 17.7 bits (34), Expect =  1935
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 1    VVSASISD 8
            VVSASISD
Sbjct: 2444 VVSASISD 2451



>NP_001152768
          Length = 257

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VVSASISDM 9
           VVS S SDM
Sbjct: 121 VVSTSTSDM 129



>NP_009181
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VVSASISDM 9
           VVS S SDM
Sbjct: 121 VVSTSTSDM 129



>NP_004215
          Length = 617

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VVSASISDM 9
          VVS S++DM
Sbjct: 67 VVSLSVADM 75



>XP_005274829
          Length = 241

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VVSASISDM 9
          VVS S++DM
Sbjct: 67 VVSLSVADM 75



>XP_006724639
          Length = 228

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VVSASISDM 9
           VVS S SDM
Sbjct: 121 VVSTSTSDM 129



>XP_006724638
          Length = 257

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VVSASISDM 9
           VVS S SDM
Sbjct: 121 VVSTSTSDM 129



>XP_005262036
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VVSASISDM 9
           VVS S SDM
Sbjct: 121 VVSTSTSDM 129



>XP_005262035
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VVSASISDM 9
           VVS S SDM
Sbjct: 121 VVSTSTSDM 129



>XP_005262034
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 7/9 (77%)

Query: 1   VVSASISDM 9
           VVS S SDM
Sbjct: 121 VVSTSTSDM 129



>NP_001489
          Length = 637

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VVSASISD 8
           V+SAS+ D
Sbjct: 299 VISASVDD 306



>NP_714921
          Length = 680

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VVSASISDM 9
           +V ASI+DM
Sbjct: 314 MVPASITDM 322



>NP_001184044
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VVSASISD 8
           V+SAS+ D
Sbjct: 261 VISASVDD 268



>XP_005271739
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VVSASISDM 9
           ++SA ++DM
Sbjct: 259 IISAGLADM 267



>NP_003469
          Length = 780

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VVSASISDM 9
           ++SA ++DM
Sbjct: 318 IISAGLADM 326



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.309    0.114    0.250 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 279,052
Number of extensions: 300
Number of successful extensions: 16
Number of sequences better than 10000.0: 16
Number of HSP's gapped: 16
Number of HSP's successfully gapped: 16
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)