Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_783864                                                              16    5631
XP_005262701                                                           16    5631
NP_944494                                                              16    7355
NP_944493                                                              16    7355
NP_002903                                                              16    7355
NP_689777                                                              16    7355
NP_060360                                                              16    7355
NP_056058                                                              16    7355
NP_004382                                                              16    7355
NP_775853                                                              16    7355
NP_919301                                                              16    7355
XP_006716574                                                           16    7355
XP_006716573                                                           16    7355
XP_005250858                                                           16    7355
XP_005250857                                                           16    7355
XP_005267148                                                           16    7355
XP_005267147                                                           16    7355
XP_006715606                                                           16    7355
NP_004106                                                              16    7355
NP_001273304                                                           15    9605
NP_000878                                                              15    9605
NP_000261                                                              15    9605
NP_073605                                                              15    9605
>NP_783864
          Length = 1044

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VRVFGFKA 9
           +RVFG+ A
Sbjct: 452 IRVFGYTA 459
>XP_005262701
          Length = 1035

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   VRVFGFKA 9
           +RVFG+ A
Sbjct: 452 IRVFGYTA 459
>NP_944494
          Length = 475

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VVRVFGFKA 9
           ++R+F FKA
Sbjct: 377 LLRMFSFKA 385
>NP_944493
          Length = 515

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VVRVFGFKA 9
           ++R+F FKA
Sbjct: 417 LLRMFSFKA 425
>NP_002903
          Length = 3130

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  VVRVFG 6
          VVRVFG
Sbjct: 37 VVRVFG 42
>NP_689777
          Length = 3997

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRVFGFKA 9
           +++FGF+A
Sbjct: 735 LKIFGFQA 742
>NP_060360
          Length = 4022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRVFGFKA 9
           +++FGF+A
Sbjct: 735 LKIFGFQA 742
>NP_056058
          Length = 863

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRVFGFKA 9
           +++FGF+A
Sbjct: 735 LKIFGFQA 742
>NP_004382
          Length = 501

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   VVRVFGFKA 9
           V++VFG+++
Sbjct: 107 VLKVFGYRS 115
>NP_775853
          Length = 622

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   RVFGFK 8
           +VFGFK
Sbjct: 278 KVFGFK 283
>NP_919301
          Length = 603

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   RVFGFKA 9
           R FGFK+
Sbjct: 493 RAFGFKS 499
>XP_006716574
          Length = 968

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRVFGFKA 9
           +++FGF+A
Sbjct: 735 LKIFGFQA 742
>XP_006716573
          Length = 1776

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRVFGFKA 9
           +++FGF+A
Sbjct: 735 LKIFGFQA 742
>XP_005250858
          Length = 4022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRVFGFKA 9
           +++FGF+A
Sbjct: 735 LKIFGFQA 742
>XP_005250857
          Length = 4022

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   VRVFGFKA 9
           +++FGF+A
Sbjct: 735 LKIFGFQA 742
>XP_005267148
          Length = 2679

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  VVRVFG 6
          VVRVFG
Sbjct: 37 VVRVFG 42
>XP_005267147
          Length = 2689

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  VVRVFG 6
          VVRVFG
Sbjct: 37 VVRVFG 42
>XP_006715606
          Length = 3130

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 1  VVRVFG 6
          VVRVFG
Sbjct: 37 VVRVFG 42
>NP_004106
          Length = 247

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  VRVFGFK 8
          VR+FG K
Sbjct: 51 VRIFGLK 57
>NP_001273304
          Length = 1169

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   VRVFGFKA 9
           VRV G KA
Sbjct: 703 VRVLGLKA 710
>NP_000878
          Length = 1163

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2   VRVFGFKA 9
           VRV G KA
Sbjct: 703 VRVLGLKA 710
>NP_000261
          Length = 289

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   VRVFGF 7
           +RVFGF
Sbjct: 233 LRVFGF 238
>NP_073605
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VVRVFGFK 8
           +VR FGF+
Sbjct: 952 IVRGFGFQ 959
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.348    0.158    0.466 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 390,969
Number of extensions: 349
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.7 bits)
S2: 28 (15.4 bits)

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