Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              18    1135
XP_006716142                                                           18    1135
XP_005250088                                                           18    1135
XP_006716141                                                           18    1135
XP_005250085                                                           18    1135
XP_006716140                                                           18    1135
XP_005250084                                                           18    1135
XP_005250083                                                           18    1135
XP_005250082                                                           18    1135
NP_848695                                                              18    1482
NP_848696                                                              18    1482
NP_110416                                                              18    1482
XP_005260619                                                           18    1482
NP_001245219                                                           17    3301
NP_775773                                                              17    3301
NP_001245220                                                           17    3301
XP_006714616                                                           17    3301
NP_443142                                                              17    4312
NP_001268432                                                           17    4312
NP_001136269                                                           16    5631
NP_001299                                                              16    5631
NP_001276932                                                           16    7355
NP_079229                                                              16    7355
NP_001130008                                                           16    7355
NP_001136438                                                           16    7355
NP_001164171                                                           16    7355
XP_006722611                                                           16    7355
XP_006722610                                                           16    7355
XP_005253995                                                           16    7355
XP_006719669                                                           16    7355
XP_006714077                                                           16    7355
XP_005247794                                                           16    7355
XP_006713802                                                           16    7355
XP_006713801                                                           16    7355
NP_899231                                                              16    7355
NP_002832                                                              15    9605
NP_005493                                                              15    9605
NP_001271254                                                           15    9605
NP_001181                                                              15    9605
NP_001158245                                                           15    9605
NP_001171500                                                           15    9605
NP_001910                                                              15    9605
NP_113666                                                              15    9605
NP_065692                                                              15    9605
NP_001155057                                                           15    9605
XP_005251837                                                           15    9605
XP_005251835                                                           15    9605
XP_006717058                                                           15    9605
XP_005251833                                                           15    9605
XP_005251831                                                           15    9605
XP_005251830                                                           15    9605
XP_005265410                                                           15    9605
XP_005265409                                                           15    9605
NP_001088                                                              15    9605
>NP_733751
          Length = 4911

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VVLPGV TA
Sbjct: 919 VVLPGVSTA 927
>XP_006716142
          Length = 4927

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VVLPGV TA
Sbjct: 920 VVLPGVSTA 928
>XP_005250088
          Length = 4928

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VVLPGV TA
Sbjct: 920 VVLPGVSTA 928
>XP_006716141
          Length = 4959

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VVLPGV TA
Sbjct: 920 VVLPGVSTA 928
>XP_005250085
          Length = 4979

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VVLPGV TA
Sbjct: 920 VVLPGVSTA 928
>XP_006716140
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VVLPGV TA
Sbjct: 920 VVLPGVSTA 928
>XP_005250084
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VVLPGV TA
Sbjct: 920 VVLPGVSTA 928
>XP_005250083
          Length = 4982

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VVLPGV TA
Sbjct: 919 VVLPGVSTA 927
>XP_005250082
          Length = 4983

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VVLPGV TA
Sbjct: 920 VVLPGVSTA 928
>NP_848695
          Length = 394

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VV+PG+F A
Sbjct: 266 VVIPGIFIA 274
>NP_848696
          Length = 426

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VV+PG+F A
Sbjct: 266 VVIPGIFIA 274
>NP_110416
          Length = 377

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VV+PG+F A
Sbjct: 266 VVIPGIFIA 274
>XP_005260619
          Length = 317

 Score = 18.1 bits (35), Expect =  1482
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           VV+PG+F A
Sbjct: 266 VVIPGIFIA 274
>NP_001245219
          Length = 850

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   PGVFTA 9
           PGVFTA
Sbjct: 273 PGVFTA 278
>NP_775773
          Length = 855

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   PGVFTA 9
           PGVFTA
Sbjct: 273 PGVFTA 278
>NP_001245220
          Length = 752

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   PGVFTA 9
           PGVFTA
Sbjct: 273 PGVFTA 278
>XP_006714616
          Length = 399

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VVLPGVF 7
           V++PGVF
Sbjct: 364 VIIPGVF 370
>NP_443142
          Length = 696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VVLPGVFTA 9
           ++LPG F A
Sbjct: 461 LILPGTFNA 469
>NP_001268432
          Length = 696

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VVLPGVFTA 9
           ++LPG F A
Sbjct: 461 LILPGTFNA 469
>NP_001136269
          Length = 415

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3  LPGVFTA 9
          LPG++TA
Sbjct: 36 LPGLYTA 42
>NP_001299
          Length = 458

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VVLPGVFT 8
           ++LPG++T
Sbjct: 379 LLLPGIYT 386
>NP_001276932
          Length = 493

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  LPGVFTA 9
          LPG FT+
Sbjct: 32 LPGAFTS 38
>NP_079229
          Length = 972

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           +++P VFT+
Sbjct: 881 IIMPPVFTS 889
>NP_001130008
          Length = 616

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   PGVFTA 9
           PGVFT+
Sbjct: 164 PGVFTS 169
>NP_001136438
          Length = 1923

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    VVLPGVF 7
            +VLPG+F
Sbjct: 1567 LVLPGMF 1573
>NP_001164171
          Length = 781

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   PGVFTA 9
           PGVFT+
Sbjct: 329 PGVFTS 334
>XP_006722611
          Length = 1034

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VVLPGVF 7
           +VLPG+F
Sbjct: 678 LVLPGMF 684
>XP_006722610
          Length = 1967

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    VVLPGVF 7
            +VLPG+F
Sbjct: 1611 LVLPGMF 1617
>XP_005253995
          Length = 836

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           +++P VFT+
Sbjct: 745 IIMPPVFTS 753
>XP_006719669
          Length = 944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VVLPGVFTA 9
           +++P VFT+
Sbjct: 853 IIMPPVFTS 861
>XP_006714077
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   PGVFTA 9
           PGVFT+
Sbjct: 454 PGVFTS 459
>XP_005247794
          Length = 518

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  LPGVFTA 9
          LPG FT+
Sbjct: 57 LPGAFTS 63
>XP_006713802
          Length = 520

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  LPGVFTA 9
          LPG FT+
Sbjct: 59 LPGAFTS 65
>XP_006713801
          Length = 520

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3  LPGVFTA 9
          LPG FT+
Sbjct: 59 LPGAFTS 65
>NP_899231
          Length = 328

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   LPGVFT 8
           LPGV+T
Sbjct: 248 LPGVYT 253
>NP_002832
          Length = 1445

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    PGVFT 8
            PGVFT
Sbjct: 1391 PGVFT 1395
>NP_005493
          Length = 2261

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    VVLPGVF 7
            +VLP VF
Sbjct: 1351 IVLPAVF 1357
>NP_001271254
          Length = 352

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VVLPGV 6
           V+LPGV
Sbjct: 251 VILPGV 256
>NP_001181
          Length = 392

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VVLPGV 6
           V+LPGV
Sbjct: 291 VILPGV 296
>NP_001158245
          Length = 300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VVLPGV 6
           V+LPGV
Sbjct: 199 VILPGV 204
>NP_001171500
          Length = 285

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   PGVFTA 9
           PGVF+A
Sbjct: 101 PGVFSA 106
>NP_001910
          Length = 302

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   PGVFTA 9
           PGVF+A
Sbjct: 101 PGVFSA 106
>NP_113666
          Length = 274

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1  VVLPGVF 7
          VV PG+F
Sbjct: 10 VVFPGLF 16
>NP_065692
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  VVLPGVFT 8
          + +PGVF+
Sbjct: 7  IFIPGVFS 14
>NP_001155057
          Length = 265

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  VVLPGVFT 8
          + +PGVF+
Sbjct: 7  IFIPGVFS 14
>XP_005251837
          Length = 1659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    VVLPGVF 7
            +VLP VF
Sbjct: 1353 IVLPAVF 1359
>XP_005251835
          Length = 2074

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    VVLPGVF 7
            +VLP VF
Sbjct: 1353 IVLPAVF 1359
>XP_006717058
          Length = 2100

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    VVLPGVF 7
            +VLP VF
Sbjct: 1353 IVLPAVF 1359
>XP_005251833
          Length = 2203

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    VVLPGVF 7
            +VLP VF
Sbjct: 1293 IVLPAVF 1299
>XP_005251831
          Length = 2263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    VVLPGVF 7
            +VLP VF
Sbjct: 1353 IVLPAVF 1359
>XP_005251830
          Length = 2263

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 1    VVLPGVF 7
            +VLP VF
Sbjct: 1353 IVLPAVF 1359
>XP_005265410
          Length = 1416

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    PGVFT 8
            PGVFT
Sbjct: 1362 PGVFT 1366
>XP_005265409
          Length = 1431

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4    PGVFT 8
            PGVFT
Sbjct: 1377 PGVFT 1381
>NP_001088
          Length = 421

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   PGVFTA 9
           PG++TA
Sbjct: 272 PGIYTA 277
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.149    0.446 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 755,031
Number of extensions: 1048
Number of successful extensions: 54
Number of sequences better than 10000.0: 54
Number of HSP's gapped: 54
Number of HSP's successfully gapped: 54
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)

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