Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              18    1482
NP_653080                                                              18    1482
XP_005267292                                                           18    1482
XP_005267291                                                           18    1482
XP_005267290                                                           18    1482
NP_004583                                                              16    5631
NP_001248340                                                           16    5631
NP_653297                                                              16    5631
NP_004288                                                              16    5631
NP_002058                                                              16    5631
NP_002063                                                              16    5631
NP_004247                                                              16    5631
XP_006719621                                                           16    5631
XP_006711224                                                           16    5631
XP_006711223                                                           16    5631
XP_006711222                                                           16    5631
XP_006711221                                                           16    5631
XP_006711220                                                           16    5631
NP_055873                                                              16    7355
NP_005883                                                              16    7355
NP_079130                                                              16    7355
XP_006722133                                                           16    7355
XP_006722132                                                           16    7355
XP_006722131                                                           16    7355
XP_006722130                                                           16    7355
XP_006722129                                                           16    7355
XP_006722128                                                           16    7355
XP_006722127                                                           16    7355
XP_006722126                                                           16    7355
XP_006722125                                                           16    7355
XP_006719854                                                           16    7355
XP_006719853                                                           16    7355
NP_078838                                                              15    9605
>NP_005091
          Length = 1782

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLLSEYDA 9
           VV LSEYDA
Sbjct: 846 VVPLSEYDA 854
>NP_653080
          Length = 1684

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLLSEYDA 9
           VV LSEYDA
Sbjct: 748 VVPLSEYDA 756
>XP_005267292
          Length = 1677

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLLSEYDA 9
           VV LSEYDA
Sbjct: 741 VVPLSEYDA 749
>XP_005267291
          Length = 1684

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLLSEYDA 9
           VV LSEYDA
Sbjct: 748 VVPLSEYDA 756
>XP_005267290
          Length = 1782

 Score = 18.1 bits (35), Expect =  1482
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VVLLSEYDA 9
           VV LSEYDA
Sbjct: 846 VVPLSEYDA 854
>NP_004583
          Length = 951

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LSEYDA 9
          LSEYDA
Sbjct: 83 LSEYDA 88
>NP_001248340
          Length = 1003

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LSEYDA 9
          LSEYDA
Sbjct: 83 LSEYDA 88
>NP_653297
          Length = 1012

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VLLSEYD 8
           VLLS+YD
Sbjct: 534 VLLSKYD 540
>NP_004288
          Length = 355

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VVLLSEYD 8
           +V LSEYD
Sbjct: 225 LVALSEYD 232
>NP_002058
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VVLLSEYD 8
           +V LSEYD
Sbjct: 229 LVALSEYD 236
>NP_002063
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VVLLSEYD 8
           +V LSEYD
Sbjct: 229 LVALSEYD 236
>NP_004247
          Length = 551

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VVLLSEYDA 9
           V+LL EY A
Sbjct: 470 VILLGEYHA 478
>XP_006719621
          Length = 874

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4  LSEYDA 9
          LSEYDA
Sbjct: 83 LSEYDA 88
>XP_006711224
          Length = 796

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VLLSEYD 8
           VLLS+YD
Sbjct: 534 VLLSKYD 540
>XP_006711223
          Length = 913

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VLLSEYD 8
           VLLS+YD
Sbjct: 534 VLLSKYD 540
>XP_006711222
          Length = 970

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VLLSEYD 8
           VLLS+YD
Sbjct: 492 VLLSKYD 498
>XP_006711221
          Length = 976

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VLLSEYD 8
           VLLS+YD
Sbjct: 534 VLLSKYD 540
>XP_006711220
          Length = 1012

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   VLLSEYD 8
           VLLS+YD
Sbjct: 534 VLLSKYD 540
>NP_055873
          Length = 1905

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VLLSEYDA 9
            + +SEYDA
Sbjct: 1630 IQMSEYDA 1637
>NP_005883
          Length = 1100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VVLLSEYD 8
          V LLS+YD
Sbjct: 53 VQLLSQYD 60
>NP_079130
          Length = 500

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VVLLSEYDA 9
           VVLL +Y+A
Sbjct: 419 VVLLGKYNA 427
>XP_006722133
          Length = 1104

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VVLLSEYD 8
          V LLS+YD
Sbjct: 53 VQLLSQYD 60
>XP_006722132
          Length = 1076

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VVLLSEYD 8
          V LLS+YD
Sbjct: 53 VQLLSQYD 60
>XP_006722131
          Length = 1100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VVLLSEYD 8
          V LLS+YD
Sbjct: 53 VQLLSQYD 60
>XP_006722130
          Length = 1106

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VVLLSEYD 8
          V LLS+YD
Sbjct: 53 VQLLSQYD 60
>XP_006722129
          Length = 1110

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VVLLSEYD 8
          V LLS+YD
Sbjct: 53 VQLLSQYD 60
>XP_006722128
          Length = 1148

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VVLLSEYD 8
          V LLS+YD
Sbjct: 53 VQLLSQYD 60
>XP_006722127
          Length = 1154

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VVLLSEYD 8
          V LLS+YD
Sbjct: 53 VQLLSQYD 60
>XP_006722126
          Length = 1158

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VVLLSEYD 8
          V LLS+YD
Sbjct: 53 VQLLSQYD 60
>XP_006722125
          Length = 1164

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VVLLSEYD 8
          V LLS+YD
Sbjct: 53 VQLLSQYD 60
>XP_006719854
          Length = 1550

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VLLSEYDA 9
            + +SEYDA
Sbjct: 1275 IQMSEYDA 1282
>XP_006719853
          Length = 1809

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2    VLLSEYDA 9
            + +SEYDA
Sbjct: 1534 IQMSEYDA 1541
>NP_078838
          Length = 1094

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2   VLLSEYD 8
           VLLS YD
Sbjct: 842 VLLSAYD 848
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.317    0.136    0.341 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 445,833
Number of extensions: 473
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.7 bits)
S2: 28 (15.4 bits)

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