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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_065681                                                              21     229
NP_066124                                                              21     229
XP_006717999                                                           21     229
NP_003204                                                              17    2528
NP_958851                                                              17    2528
NP_109592                                                              15    9605
NP_002839                                                              15    9605

>NP_065681
          Length = 1072

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VVKATAFHM 9
           VVKATAFH+
Sbjct: 738 VVKATAFHL 746



>NP_066124
          Length = 1114

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VVKATAFHM 9
           VVKATAFH+
Sbjct: 738 VVKATAFHL 746



>XP_006717999
          Length = 1207

 Score = 20.8 bits (42), Expect =   229
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VVKATAFHM 9
           VVKATAFH+
Sbjct: 831 VVKATAFHL 839



>NP_003204
          Length = 434

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VVKATAFH 8
           ++ ATAFH
Sbjct: 138 IISATAFH 145



>NP_958851
          Length = 305

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1  VVKATAFH 8
          ++ ATAFH
Sbjct: 9  IISATAFH 16



>NP_109592
          Length = 1216

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  ATAFHM 9
          ATAFH+
Sbjct: 27 ATAFHV 32



>NP_002839
          Length = 1188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  ATAFHM 9
          ATAFH+
Sbjct: 27 ATAFHV 32



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.339    0.129    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 303,251
Number of extensions: 183
Number of successful extensions: 7
Number of sequences better than 10000.0: 7
Number of HSP's gapped: 7
Number of HSP's successfully gapped: 7
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.6 bits)
S2: 28 (15.4 bits)