Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001245200                                                           19     869
NP_000240                                                              19     869
NP_001161089                                                           19     869
XP_005265218                                                           19     869
NP_006419                                                              18    1935
XP_005255098                                                           18    1935
XP_005255097                                                           18    1935
NP_002205                                                              17    3301
NP_056083                                                              17    3301
XP_005249809                                                           17    3301
XP_005249808                                                           17    3301
XP_005247302                                                           17    3301
NP_001257292                                                           16    5631
NP_001257291                                                           16    5631
NP_005044                                                              16    5631
NP_036519                                                              16    5631
NP_055026                                                              16    7355
NP_068838                                                              16    7355
XP_005262246                                                           16    7355
XP_005262245                                                           16    7355
XP_005262244                                                           16    7355
XP_006724750                                                           16    7355
XP_005262243                                                           16    7355
XP_005249007                                                           16    7355
XP_005265929                                                           16    7355
NP_001012458                                                           16    7355
NP_001234916                                                           15    9605
NP_065877                                                              15    9605
XP_006722374                                                           15    9605
XP_006722373                                                           15    9605
XP_006722370                                                           15    9605
XP_006722365                                                           15    9605
XP_006718330                                                           15    9605
>NP_001245200
          Length = 687

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VTITMKTAD 9
           VTIT KTAD
Sbjct: 113 VTITTKTAD 121
>NP_000240
          Length = 756

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VTITMKTAD 9
           VTIT KTAD
Sbjct: 113 VTITTKTAD 121
>NP_001161089
          Length = 658

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VTITMKTAD 9
          VTIT KTAD
Sbjct: 15 VTITTKTAD 23
>XP_005265218
          Length = 687

 Score = 18.9 bits (37), Expect =   869
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VTITMKTAD 9
           VTIT KTAD
Sbjct: 113 VTITTKTAD 121
>NP_006419
          Length = 256

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTITMKTAD 9
           VT+TM+T D
Sbjct: 125 VTVTMRTLD 133
>XP_005255098
          Length = 256

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTITMKTAD 9
           VT+TM+T D
Sbjct: 125 VTVTMRTLD 133
>XP_005255097
          Length = 287

 Score = 17.7 bits (34), Expect =  1935
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VTITMKTAD 9
           VT+TM+T D
Sbjct: 156 VTVTMRTLD 164
>NP_002205
          Length = 769

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VTITMKTAD 9
           VT+TMK  D
Sbjct: 432 VTVTMKKCD 440
>NP_056083
          Length = 2243

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    TITMKTAD 9
            TITM+T+D
Sbjct: 1401 TITMETSD 1408
>XP_005249809
          Length = 634

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VTITMKTAD 9
           VT+TMK  D
Sbjct: 297 VTVTMKKCD 305
>XP_005249808
          Length = 634

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1   VTITMKTAD 9
           VT+TMK  D
Sbjct: 297 VTVTMKKCD 305
>XP_005247302
          Length = 2248

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2    TITMKTAD 9
            TITM+T+D
Sbjct: 1406 TITMETSD 1413
>NP_001257292
          Length = 308

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1 VTITMKT 7
         VTIT+KT
Sbjct: 3 VTITLKT 9
>NP_001257291
          Length = 362

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1 VTITMKT 7
         VTIT+KT
Sbjct: 3 VTITLKT 9
>NP_005044
          Length = 363

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1 VTITMKT 7
         VTIT+KT
Sbjct: 3 VTITLKT 9
>NP_036519
          Length = 663

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VTITMKTA 8
          VT+TMK A
Sbjct: 76 VTLTMKVA 83
>NP_055026
          Length = 440

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ITMKTAD 9
           +TMKT+D
Sbjct: 383 LTMKTSD 389
>NP_068838
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TITMKTAD 9
           T+T+KT D
Sbjct: 183 TVTIKTED 190
>XP_005262246
          Length = 761

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TITMKTAD 9
           T+T+KT D
Sbjct: 183 TVTIKTED 190
>XP_005262245
          Length = 807

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TITMKTAD 9
           T+T+KT D
Sbjct: 183 TVTIKTED 190
>XP_005262244
          Length = 807

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TITMKTAD 9
           T+T+KT D
Sbjct: 183 TVTIKTED 190
>XP_006724750
          Length = 807

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TITMKTAD 9
           T+T+KT D
Sbjct: 183 TVTIKTED 190
>XP_005262243
          Length = 807

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   TITMKTAD 9
           T+T+KT D
Sbjct: 183 TVTIKTED 190
>XP_005249007
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  VTITMKTAD 9
          +T+T++TA+
Sbjct: 3  ITLTLQTAE 11
>XP_005265929
          Length = 361

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   ITMKTAD 9
           +TMKT+D
Sbjct: 304 LTMKTSD 310
>NP_001012458
          Length = 389

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1  VTITMKTAD 9
          +T+T++TA+
Sbjct: 3  ITLTLQTAE 11
>NP_001234916
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VTITMKTAD 9
           +T+T++T D
Sbjct: 95  LTLTLRTCD 103
>NP_065877
          Length = 426

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VTITMKTAD 9
           +T+T++T D
Sbjct: 239 LTLTLRTCD 247
>XP_006722374
          Length = 944

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VTITMKTAD 9
           VT++ KT D
Sbjct: 710 VTVSSKTGD 718
>XP_006722373
          Length = 948

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VTITMKTAD 9
           VT++ KT D
Sbjct: 714 VTVSSKTGD 722
>XP_006722370
          Length = 956

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VTITMKTAD 9
           VT++ KT D
Sbjct: 722 VTVSSKTGD 730
>XP_006722365
          Length = 981

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VTITMKTAD 9
           VT++ KT D
Sbjct: 747 VTVSSKTGD 755
>XP_006718330
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VTITMKTAD 9
           +T+T++T D
Sbjct: 95  LTLTLRTCD 103
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.314    0.118    0.290 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 302,047
Number of extensions: 501
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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