Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_006501                                                              18    1135
NP_003974                                                              17    3301
NP_001070253                                                           17    3301
NP_008970                                                              16    5631
NP_058641                                                              16    5631
XP_005267344                                                           16    5631
NP_001119577                                                           16    7355
NP_001119578                                                           16    7355
NP_078858                                                              16    7355
NP_001278232                                                           16    7355
NP_001278214                                                           16    7355
NP_001137420                                                           16    7355
NP_116133                                                              16    7355
NP_002604                                                              16    7355
NP_001248745                                                           16    7355
NP_001257625                                                           16    7355
NP_001257624                                                           16    7355
NP_060757                                                              16    7355
NP_078904                                                              16    7355
NP_001182412                                                           16    7355
XP_005255413                                                           16    7355
XP_006720365                                                           16    7355
XP_006720074                                                           16    7355
XP_006717604                                                           16    7355
XP_006717603                                                           16    7355
XP_005249511                                                           16    7355
XP_005275825                                                           16    7355
XP_003960932                                                           16    7355
XP_006710060                                                           16    7355
NP_115758                                                              15    9605
NP_570629                                                              15    9605
NP_001034439                                                           15    9605
NP_001927                                                              15    9605
NP_001277181                                                           15    9605
XP_006715935                                                           15    9605
XP_006715934                                                           15    9605
XP_006710661                                                           15    9605
XP_006710659                                                           15    9605
XP_006710658                                                           15    9605
XP_006710657                                                           15    9605
NP_001053                                                              15    9605
NP_001968                                                              15    9605
>NP_006501
          Length = 513

 Score = 18.5 bits (36), Expect =  1135
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   YFSLSYS 9
           YFSLSYS
Sbjct: 473 YFSLSYS 479
>NP_003974
          Length = 515

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VSYFSLSY 8
           ++YFS SY
Sbjct: 390 INYFSFSY 397
>NP_001070253
          Length = 515

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VSYFSLSY 8
           ++YFS SY
Sbjct: 390 INYFSFSY 397
>NP_008970
          Length = 1174

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VSYFSLSY 8
          V+YFSL Y
Sbjct: 61 VTYFSLWY 68

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FSLSYS 9
           FSLSYS
Sbjct: 512 FSLSYS 517
>NP_058641
          Length = 291

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2  SYFSLSY 8
          SYF+L+Y
Sbjct: 70 SYFNLNY 76
>XP_005267344
          Length = 1174

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VSYFSLSY 8
          V+YFSL Y
Sbjct: 61 VTYFSLWY 68

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FSLSYS 9
           FSLSYS
Sbjct: 512 FSLSYS 517
>NP_001119577
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VSYFSLSY 8
           ++Y+S SY
Sbjct: 382 INYYSFSY 389
>NP_001119578
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VSYFSLSY 8
           ++Y+S SY
Sbjct: 382 INYYSFSY 389
>NP_078858
          Length = 4981

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SYFSLS 7
            SYFSLS
Sbjct: 3556 SYFSLS 3561
>NP_001278232
          Length = 4983

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SYFSLS 7
            SYFSLS
Sbjct: 3558 SYFSLS 3563
>NP_001278214
          Length = 4982

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2    SYFSLS 7
            SYFSLS
Sbjct: 3558 SYFSLS 3563
>NP_001137420
          Length = 248

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SYFSLSY 8
           ++FSLSY
Sbjct: 213 AFFSLSY 219
>NP_116133
          Length = 230

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SYFSLSY 8
           ++FSLSY
Sbjct: 195 AFFSLSY 201
>NP_002604
          Length = 556

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YFSLSYS 9
           YF LSY+
Sbjct: 156 YFGLSYA 162
>NP_001248745
          Length = 454

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YFSLSYS 9
           YF LSY+
Sbjct: 156 YFGLSYA 162
>NP_001257625
          Length = 816

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +Y+S SYS
Sbjct: 323 NYYSTSYS 330
>NP_001257624
          Length = 841

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +Y+S SYS
Sbjct: 323 NYYSTSYS 330
>NP_060757
          Length = 846

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +Y+S SYS
Sbjct: 323 NYYSTSYS 330
>NP_078904
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SYFSLS 7
           SYFSLS
Sbjct: 401 SYFSLS 406
>NP_001182412
          Length = 714

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 2   SYFSLS 7
           SYFSLS
Sbjct: 401 SYFSLS 406
>XP_005255413
          Length = 529

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YFSLSYS 9
           YF LSY+
Sbjct: 129 YFGLSYA 135
>XP_006720365
          Length = 511

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1   VSYFSLSY 8
           ++Y+S SY
Sbjct: 382 INYYSFSY 389
>XP_006720074
          Length = 1051

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   FSLSYS 9
           FSLSYS
Sbjct: 389 FSLSYS 394
>XP_006717604
          Length = 820

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +Y+S SYS
Sbjct: 323 NYYSTSYS 330
>XP_006717603
          Length = 846

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +Y+S SYS
Sbjct: 323 NYYSTSYS 330
>XP_005249511
          Length = 230

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SYFSLSY 8
           ++FSLSY
Sbjct: 195 AFFSLSY 201
>XP_005275825
          Length = 218

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YFSLSYS 9
           YF LSY+
Sbjct: 129 YFGLSYA 135
>XP_003960932
          Length = 396

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YFSLSYS 9
           YF LSY+
Sbjct: 129 YFGLSYA 135
>XP_006710060
          Length = 425

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YFSLSYS 9
           YF LSY+
Sbjct: 129 YFGLSYA 135
>NP_115758
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   YFSLSYS 9
           YF LS+S
Sbjct: 351 YFGLSFS 357
>NP_570629
          Length = 865

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +YFS S+S
Sbjct: 537 TYFSASFS 544
>NP_001034439
          Length = 801

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +YFS S+S
Sbjct: 473 TYFSASFS 480
>NP_001927
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +YFS S+S
Sbjct: 475 TYFSASFS 482
>NP_001277181
          Length = 758

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +YFS S+S
Sbjct: 430 TYFSASFS 437
>XP_006715935
          Length = 804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +YFS S+S
Sbjct: 476 TYFSASFS 483
>XP_006715934
          Length = 804

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           +YFS S+S
Sbjct: 476 TYFSASFS 483
>XP_006710661
          Length = 4057

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    SYFSLSY 8
            S FSLSY
Sbjct: 1561 SQFSLSY 1567
>XP_006710659
          Length = 4556

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    SYFSLSY 8
            S FSLSY
Sbjct: 1537 SQFSLSY 1543
>XP_006710658
          Length = 4563

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    SYFSLSY 8
            S FSLSY
Sbjct: 1544 SQFSLSY 1550
>XP_006710657
          Length = 4579

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 2    SYFSLSY 8
            S FSLSY
Sbjct: 1560 SQFSLSY 1566
>NP_001053
          Length = 433

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SYFSLSYS 9
           SY S++YS
Sbjct: 332 SYISVNYS 339
>NP_001968
          Length = 957

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   YFSLSYS 9
           YF+++YS
Sbjct: 336 YFAMNYS 342
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.125    0.332 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 536,236
Number of extensions: 980
Number of successful extensions: 44
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 44
Number of HSP's successfully gapped: 44
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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