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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_068838                                                              17    3301
XP_005262246                                                           17    3301
XP_005262245                                                           17    3301
XP_005262244                                                           17    3301
XP_006724750                                                           17    3301
XP_005262243                                                           17    3301
NP_000622                                                              16    5631
NP_004481                                                              16    7355
NP_079216                                                              16    7355
XP_005273708                                                           16    7355
XP_006716465                                                           16    7355
XP_005273707                                                           16    7355
XP_006712520                                                           16    7355
XP_005246534                                                           16    7355
NP_004251                                                              15    9605
NP_001460                                                              15    9605
NP_001275906                                                           15    9605
NP_001275905                                                           15    9605
NP_001275907                                                           15    9605
NP_036424                                                              15    9605
NP_000258                                                              15    9605
NP_001035957                                                           15    9605
NP_001269264                                                           15    9605
NP_001245257                                                           15    9605
XP_006722776                                                           15    9605
XP_006721990                                                           15    9605
XP_006721989                                                           15    9605
XP_005258041                                                           15    9605
XP_006721988                                                           15    9605
XP_006721987                                                           15    9605
XP_006721986                                                           15    9605
XP_005258040                                                           15    9605
XP_006721985                                                           15    9605

>NP_068838
          Length = 761

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   VAIEEDL 9
           VAIEEDL
Sbjct: 98  VAIEEDL 104



>XP_005262246
          Length = 761

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   VAIEEDL 9
           VAIEEDL
Sbjct: 98  VAIEEDL 104



>XP_005262245
          Length = 807

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   VAIEEDL 9
           VAIEEDL
Sbjct: 98  VAIEEDL 104



>XP_005262244
          Length = 807

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   VAIEEDL 9
           VAIEEDL
Sbjct: 98  VAIEEDL 104



>XP_006724750
          Length = 807

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   VAIEEDL 9
           VAIEEDL
Sbjct: 98  VAIEEDL 104



>XP_005262243
          Length = 807

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 3   VAIEEDL 9
           VAIEEDL
Sbjct: 98  VAIEEDL 104



>NP_000622
          Length = 339

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   VAIEEDL 9
           +A+EEDL
Sbjct: 255 IAVEEDL 261



>NP_004481
          Length = 540

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSVAIEEDL 9
           +SVA+EE L
Sbjct: 396 LSVAVEEGL 404



>NP_079216
          Length = 1870

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VSVAIEE 7
           VS+AIEE
Sbjct: 515 VSIAIEE 521



>XP_005273708
          Length = 1032

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VSVAIEE 7
           VS+AIEE
Sbjct: 229 VSIAIEE 235



>XP_006716465
          Length = 1826

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VSVAIEE 7
           VS+AIEE
Sbjct: 515 VSIAIEE 521



>XP_005273707
          Length = 1847

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 1   VSVAIEE 7
           VS+AIEE
Sbjct: 515 VSIAIEE 521



>XP_006712520
          Length = 340

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSVAIEEDL 9
           +SVA+EE L
Sbjct: 196 LSVAVEEGL 204



>XP_005246534
          Length = 453

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSVAIEEDL 9
           +SVA+EE L
Sbjct: 309 LSVAVEEGL 317



>NP_004251
          Length = 1208

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SVAIEED 8
           +VA+EED
Sbjct: 291 AVAVEED 297



>NP_001460
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSVAIEEDL 9
           +S+A +EDL
Sbjct: 221 ISIAEDEDL 229



>NP_001275906
          Length = 568

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSVAIEEDL 9
           +S+A +EDL
Sbjct: 180 ISIAEDEDL 188



>NP_001275905
          Length = 609

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSVAIEEDL 9
           +S+A +EDL
Sbjct: 221 ISIAEDEDL 229



>NP_001275907
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSVAIEEDL 9
           +S+A +EDL
Sbjct: 171 ISIAEDEDL 179



>NP_036424
          Length = 1136

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SVAIEEDL 9
           S+A+E+DL
Sbjct: 341 SLALEQDL 348



>NP_000258
          Length = 2818

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SVAIEEDL 9
            ++A+EEDL
Sbjct: 1709 TLALEEDL 1716



>NP_001035957
          Length = 2839

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SVAIEEDL 9
            ++A+EEDL
Sbjct: 1730 TLALEEDL 1737



>NP_001269264
          Length = 1019

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SVAIEEDL 9
           S+A+E+DL
Sbjct: 224 SLALEQDL 231



>NP_001245257
          Length = 1152

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SVAIEEDL 9
           S+A+E+DL
Sbjct: 357 SLALEQDL 364



>XP_006722776
          Length = 1164

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SVAIEEDL 9
           S+A+E+DL
Sbjct: 341 SLALEQDL 348



>XP_006721990
          Length = 2823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SVAIEEDL 9
            ++A+EEDL
Sbjct: 1740 TLALEEDL 1747



>XP_006721989
          Length = 2823

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SVAIEEDL 9
            ++A+EEDL
Sbjct: 1740 TLALEEDL 1747



>XP_005258041
          Length = 2836

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SVAIEEDL 9
            ++A+EEDL
Sbjct: 1709 TLALEEDL 1716



>XP_006721988
          Length = 2846

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SVAIEEDL 9
            ++A+EEDL
Sbjct: 1719 TLALEEDL 1726



>XP_006721987
          Length = 2849

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SVAIEEDL 9
            ++A+EEDL
Sbjct: 1740 TLALEEDL 1747



>XP_006721986
          Length = 2854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SVAIEEDL 9
            ++A+EEDL
Sbjct: 1727 TLALEEDL 1734



>XP_005258040
          Length = 2857

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SVAIEEDL 9
            ++A+EEDL
Sbjct: 1730 TLALEEDL 1737



>XP_006721985
          Length = 2867

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2    SVAIEEDL 9
            ++A+EEDL
Sbjct: 1740 TLALEEDL 1747



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.306    0.129    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 624,392
Number of extensions: 646
Number of successful extensions: 33
Number of sequences better than 10000.0: 33
Number of HSP's gapped: 33
Number of HSP's successfully gapped: 33
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.3 bits)
S2: 28 (15.4 bits)