Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_037440                                                              18    1135
XP_005247460                                                           18    1135
XP_005247459                                                           18    1135
NP_036423                                                              17    2528
XP_006719768                                                           17    2528
NP_001122306                                                           17    4312
NP_000524                                                              17    4312
NP_955772                                                              17    4312
NP_569055                                                              16    5631
NP_000676                                                              16    5631
NP_033611                                                              16    5631
NP_114438                                                              16    5631
NP_004826                                                              16    5631
NP_114038                                                              16    5631
NP_000858                                                              16    5631
NP_036471                                                              16    5631
NP_001257951                                                           16    5631
NP_001257952                                                           16    5631
NP_005289                                                              16    5631
XP_005246577                                                           16    5631
XP_006712545                                                           16    5631
NP_001257906                                                           16    7355
NP_001257904                                                           16    7355
NP_001257905                                                           16    7355
NP_055576                                                              16    7355
NP_064707                                                              16    7355
NP_003244                                                              16    7355
NP_064505                                                              16    7355
NP_056996                                                              16    7355
NP_775760                                                              16    7355
NP_001001479                                                           16    7355
XP_005261097                                                           16    7355
XP_005261096                                                           16    7355
XP_005261095                                                           16    7355
XP_005261094                                                           16    7355
XP_005250985                                                           16    7355
XP_006716634                                                           16    7355
XP_006714502                                                           16    7355
XP_005246155                                                           16    7355
XP_006712703                                                           16    7355
XP_005246154                                                           16    7355
XP_006712699                                                           16    7355
XP_006712698                                                           16    7355
XP_006712697                                                           16    7355
NP_003741                                                              15    9605
NP_060831                                                              15    9605
NP_005283                                                              15    9605
NP_000557                                                              15    9605
NP_001004340                                                           15    9605
NP_001017986                                                           15    9605
XP_006720009                                                           15    9605
XP_005245015                                                           15    9605
XP_005245014                                                           15    9605
NP_001091670                                                           15    9605
NP_001135780                                                           15    9605
>NP_037440
          Length = 319

 Score = 18.5 bits (36), Expect =  1135
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VSTVIWLL 8
           +STV+WL+
Sbjct: 134 ISTVVWLM 141
>XP_005247460
          Length = 319

 Score = 18.5 bits (36), Expect =  1135
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VSTVIWLL 8
           +STV+WL+
Sbjct: 134 ISTVVWLM 141
>XP_005247459
          Length = 319

 Score = 18.5 bits (36), Expect =  1135
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 1   VSTVIWLL 8
           +STV+WL+
Sbjct: 134 ISTVVWLM 141
>NP_036423
          Length = 2120

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   STVIWLL 8
           STV+W+L
Sbjct: 432 STVVWML 438
>XP_006719768
          Length = 2120

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   STVIWLL 8
           STV+W+L
Sbjct: 432 STVVWML 438
>NP_001122306
          Length = 277

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TVIWLL 8
           TV+WLL
Sbjct: 160 TVVWLL 165
>NP_000524
          Length = 277

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TVIWLL 8
           TV+WLL
Sbjct: 160 TVVWLL 165
>NP_955772
          Length = 242

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   TVIWLL 8
           TV+WLL
Sbjct: 125 TVVWLL 130
>NP_569055
          Length = 791

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VIWLLA 9
           V+WLLA
Sbjct: 659 VVWLLA 664
>NP_000676
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VIWLLA 9
           +IWLLA
Sbjct: 151 IIWLLA 156
>NP_033611
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VIWLLA 9
           +IWLLA
Sbjct: 151 IIWLLA 156
>NP_114438
          Length = 388

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VIWLLA 9
           +IWLLA
Sbjct: 180 IIWLLA 185
>NP_004826
          Length = 394

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VIWLLA 9
           +IWLLA
Sbjct: 186 IIWLLA 191
>NP_114038
          Length = 394

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VIWLLA 9
           +IWLLA
Sbjct: 186 IIWLLA 191
>NP_000858
          Length = 481

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   TVIWLLA 9
           TV+WL++
Sbjct: 177 TVVWLIS 183
>NP_036471
          Length = 294

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  STVIWLL 8
          STV WL+
Sbjct: 28 STVFWLI 34
>NP_001257951
          Length = 664

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VIWLLA 9
           V+WLLA
Sbjct: 532 VVWLLA 537
>NP_001257952
          Length = 698

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   VIWLLA 9
           V+WLLA
Sbjct: 566 VVWLLA 571
>NP_005289
          Length = 361

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4  VIWLLA 9
          V+WLLA
Sbjct: 60 VVWLLA 65
>XP_005246577
          Length = 362

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3  TVIWLLA 9
          TV+WL++
Sbjct: 58 TVVWLIS 64
>XP_006712545
          Length = 456

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 3   TVIWLLA 9
           TV+WL++
Sbjct: 152 TVVWLIS 158
>NP_001257906
          Length = 335

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VSTVIW 6
           VST+IW
Sbjct: 100 VSTLIW 105
>NP_001257904
          Length = 366

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VSTVIW 6
           VST+IW
Sbjct: 100 VSTLIW 105
>NP_001257905
          Length = 379

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VSTVIW 6
           VST+IW
Sbjct: 113 VSTLIW 118
>NP_055576
          Length = 360

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 1   VSTVIW 6
           VST+IW
Sbjct: 100 VSTLIW 105
>NP_064707
          Length = 362

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VSTVIWLLA 9
           V  ++WLLA
Sbjct: 164 VCILVWLLA 172
>NP_003244
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    STVIWL 7
            +TVIWL
Sbjct: 1271 TTVIWL 1276
>NP_064505
          Length = 1555

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  VSTVIWLLA 9
          V TV+WL +
Sbjct: 30 VLTVLWLFS 38
>NP_056996
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    VSTVIWLL 8
            VST +WL+
Sbjct: 1261 VSTGLWLM 1268
>NP_775760
          Length = 1585

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   VSTVIW 6
           +STV+W
Sbjct: 565 ISTVLW 570
>NP_001001479
          Length = 350

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  VSTVIWLLA 9
          ++ ++WLLA
Sbjct: 51 MAALVWLLA 59
>XP_005261097
          Length = 1452

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    STVIWL 7
            +TVIWL
Sbjct: 1271 TTVIWL 1276
>XP_005261096
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    STVIWL 7
            +TVIWL
Sbjct: 1271 TTVIWL 1276
>XP_005261095
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    STVIWL 7
            +TVIWL
Sbjct: 1271 TTVIWL 1276
>XP_005261094
          Length = 1591

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2    STVIWL 7
            +TVIWL
Sbjct: 1271 TTVIWL 1276
>XP_005250985
          Length = 1406

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    VSTVIWLL 8
            VST +WL+
Sbjct: 1261 VSTGLWLM 1268
>XP_006716634
          Length = 1420

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1    VSTVIWLL 8
            VST +WL+
Sbjct: 1275 VSTGLWLM 1282
>XP_006714502
          Length = 1064

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  VSTVIW 6
          +STV+W
Sbjct: 48 ISTVLW 53
>XP_005246155
          Length = 362

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VSTVIWLLA 9
           V  ++WLLA
Sbjct: 164 VCILVWLLA 172
>XP_006712703
          Length = 362

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VSTVIWLLA 9
           V  ++WLLA
Sbjct: 164 VCILVWLLA 172
>XP_005246154
          Length = 362

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1   VSTVIWLLA 9
           V  ++WLLA
Sbjct: 164 VCILVWLLA 172
>XP_006712699
          Length = 1544

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  VSTVIWLLA 9
          V TV+WL +
Sbjct: 19 VLTVLWLFS 27
>XP_006712698
          Length = 1547

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  VSTVIWLLA 9
          V TV+WL +
Sbjct: 30 VLTVLWLFS 38
>XP_006712697
          Length = 1549

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1  VSTVIWLLA 9
          V TV+WL +
Sbjct: 24 VLTVLWLFS 32
>NP_003741
          Length = 1382

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 5/6 (83%)

Query: 1   VSTVIW 6
           VSTV W
Sbjct: 279 VSTVFW 284
>NP_060831
          Length = 364

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   STVIWLLA 9
           STV W++A
Sbjct: 95  STVDWIVA 102
>NP_005283
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   STVIWLLA 9
           S  +WLLA
Sbjct: 106 SIALWLLA 113
>NP_000557
          Length = 374

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VSTVIWL 7
           V+TV+W+
Sbjct: 305 VNTVLWV 311
>NP_001004340
          Length = 188

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VSTVIWL 7
           V+TV+W+
Sbjct: 119 VNTVLWV 125
>NP_001017986
          Length = 280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VSTVIWL 7
           V+TV+W+
Sbjct: 211 VNTVLWV 217
>XP_006720009
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   STVIWLLA 9
           S  +WLLA
Sbjct: 106 SIALWLLA 113
>XP_005245015
          Length = 282

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VSTVIWL 7
           V+TV+W+
Sbjct: 213 VNTVLWV 219
>XP_005245014
          Length = 375

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/7 (57%), Positives = 7/7 (100%)

Query: 1   VSTVIWL 7
           V+TV+W+
Sbjct: 306 VNTVLWV 312
>NP_001091670
          Length = 331

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 6/8 (75%)

Query: 2   STVIWLLA 9
           S  +WLLA
Sbjct: 106 SIALWLLA 113
>NP_001135780
          Length = 2156

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 2   STVIW 6
           STVIW
Sbjct: 760 STVIW 764
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.331    0.133    0.453 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 574,100
Number of extensions: 563
Number of successful extensions: 55
Number of sequences better than 10000.0: 55
Number of HSP's gapped: 55
Number of HSP's successfully gapped: 55
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)

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