Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_004324                                                              18    1135
XP_005250103                                                           18    1135
XP_005250102                                                           18    1135
NP_001035194                                                           17    3301
NP_659410                                                              17    4312
XP_006719103                                                           17    4312
XP_005253384                                                           17    4312
XP_005253383                                                           17    4312
NP_065784                                                              16    5631
XP_005247723                                                           16    5631
NP_001273598                                                           15    9605
NP_001273599                                                           15    9605
NP_861522                                                              15    9605
NP_001273597                                                           15    9605
NP_001269973                                                           15    9605
NP_065094                                                              15    9605
NP_055805                                                              15    9605
NP_001269972                                                           15    9605
NP_110436                                                              15    9605
NP_919277                                                              15    9605
XP_005267555                                                           15    9605
XP_006720167                                                           15    9605
XP_006720166                                                           15    9605
XP_005267550                                                           15    9605
XP_005267549                                                           15    9605
XP_005267548                                                           15    9605
XP_006720165                                                           15    9605
XP_006720164                                                           15    9605
XP_006720163                                                           15    9605
XP_006720162                                                           15    9605
XP_006720161                                                           15    9605
XP_006719684                                                           15    9605
XP_005265784                                                           15    9605
XP_005265778                                                           15    9605
>NP_004324
          Length = 766

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VSSSASMDS 9
           VSSSASMD+
Sbjct: 111 VSSSASMDT 119
>XP_005250103
          Length = 758

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VSSSASMDS 9
           VSSSASMD+
Sbjct: 111 VSSSASMDT 119
>XP_005250102
          Length = 767

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VSSSASMDS 9
           VSSSASMD+
Sbjct: 111 VSSSASMDT 119
>NP_001035194
          Length = 4493

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 9/9 (100%)

Query: 1    VSSSASMDS 9
            +SSSA++DS
Sbjct: 3732 ISSSATLDS 3740
>NP_659410
          Length = 1274

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SSSASMDS 9
           SSS SMDS
Sbjct: 150 SSSCSMDS 157
>XP_006719103
          Length = 1296

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SSSASMDS 9
           SSS SMDS
Sbjct: 172 SSSCSMDS 179
>XP_005253384
          Length = 1311

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SSSASMDS 9
           SSS SMDS
Sbjct: 150 SSSCSMDS 157
>XP_005253383
          Length = 1333

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 2   SSSASMDS 9
           SSS SMDS
Sbjct: 172 SSSCSMDS 179
>NP_065784
          Length = 1381

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1   VSSSASMDS 9
           VSSSAS++S
Sbjct: 980 VSSSASVNS 988
>XP_005247723
          Length = 1481

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1    VSSSASMDS 9
            VSSSAS++S
Sbjct: 1080 VSSSASVNS 1088
>NP_001273598
          Length = 945

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSSSASMD 8
           V SSA+MD
Sbjct: 703 VKSSATMD 710
>NP_001273599
          Length = 613

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSSSASMD 8
           V SSA+MD
Sbjct: 371 VKSSATMD 378
>NP_861522
          Length = 1098

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSSSASMD 8
           V SSA+MD
Sbjct: 856 VKSSATMD 863
>NP_001273597
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSSSASMD 8
           V SSA+MD
Sbjct: 756 VKSSATMD 763
>NP_001269973
          Length = 2083

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSSSASMDS 9
           V+SSA +DS
Sbjct: 982 VTSSADVDS 990
>NP_065094
          Length = 437

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSSSASMD 8
           VSS +SMD
Sbjct: 377 VSSGSSMD 384
>NP_055805
          Length = 2036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSSSASMDS 9
           V+SSA +DS
Sbjct: 935 VTSSADVDS 943
>NP_001269972
          Length = 2049

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSSSASMDS 9
           V+SSA +DS
Sbjct: 948 VTSSADVDS 956
>NP_110436
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SSASMDS 9
           SSAS+DS
Sbjct: 345 SSASLDS 351
>NP_919277
          Length = 2083

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSSSASMDS 9
           V+SSA +DS
Sbjct: 935 VTSSADVDS 943
>XP_005267555
          Length = 1325

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSSSASMDS 9
           V+SSA +DS
Sbjct: 176 VTSSADVDS 184
>XP_006720167
          Length = 1607

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VSSSASMDS 9
            V+SSA +DS
Sbjct: 1441 VTSSADVDS 1449
>XP_006720166
          Length = 2083

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSSSASMDS 9
           V+SSA +DS
Sbjct: 982 VTSSADVDS 990
>XP_005267550
          Length = 2084

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSSSASMDS 9
           V+SSA +DS
Sbjct: 935 VTSSADVDS 943
>XP_005267549
          Length = 2130

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSSSASMDS 9
           V+SSA +DS
Sbjct: 982 VTSSADVDS 990
>XP_005267548
          Length = 2131

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSSSASMDS 9
           V+SSA +DS
Sbjct: 982 VTSSADVDS 990
>XP_006720165
          Length = 2542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VSSSASMDS 9
            V+SSA +DS
Sbjct: 1441 VTSSADVDS 1449
>XP_006720164
          Length = 2542

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VSSSASMDS 9
            V+SSA +DS
Sbjct: 1441 VTSSADVDS 1449
>XP_006720163
          Length = 2543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VSSSASMDS 9
            V+SSA +DS
Sbjct: 1394 VTSSADVDS 1402
>XP_006720162
          Length = 2589

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VSSSASMDS 9
            V+SSA +DS
Sbjct: 1441 VTSSADVDS 1449
>XP_006720161
          Length = 2590

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1    VSSSASMDS 9
            V+SSA +DS
Sbjct: 1441 VTSSADVDS 1449
>XP_006719684
          Length = 543

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   SSASMDS 9
           SSAS+DS
Sbjct: 345 SSASLDS 351
>XP_005265784
          Length = 721

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSSSASMD 8
           V SSA+MD
Sbjct: 479 VKSSATMD 486
>XP_005265778
          Length = 1098

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VSSSASMD 8
           V SSA+MD
Sbjct: 856 VKSSATMD 863
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.274   0.0837    0.168 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 327,762
Number of extensions: 469
Number of successful extensions: 34
Number of sequences better than 10000.0: 34
Number of HSP's gapped: 34
Number of HSP's successfully gapped: 34
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.6 bits)
S2: 28 (15.4 bits)

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