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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_689716                                                              17    4312
NP_004441                                                              16    7355
NP_059980                                                              16    7355
NP_733793                                                              15    9605
NP_004832                                                              15    9605
XP_005245679                                                           15    9605
XP_005245678                                                           15    9605
XP_005245677                                                           15    9605
XP_006711697                                                           15    9605

>NP_689716
          Length = 1052

 Score = 16.5 bits (31), Expect =  4312
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2    SRTLLLVQ 9
            S+TLLLVQ
Sbjct: 1021 SQTLLLVQ 1028



>NP_004441
          Length = 104

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1 VSRTLLLVQ 9
         +S T+LLVQ
Sbjct: 1 MSHTILLVQ 9



>NP_059980
          Length = 235

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/8 (87%), Positives = 7/8 (87%)

Query: 1  VSRTLLLV 8
          V RTLLLV
Sbjct: 21 VMRTLLLV 28



>NP_733793
          Length = 1280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RTLLLVQ 9
           +TLLLVQ
Sbjct: 817 KTLLLVQ 823



>NP_004832
          Length = 1139

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RTLLLVQ 9
           +TLLLVQ
Sbjct: 676 KTLLLVQ 682



>XP_005245679
          Length = 1142

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RTLLLVQ 9
           +TLLLVQ
Sbjct: 676 KTLLLVQ 682



>XP_005245678
          Length = 1145

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RTLLLVQ 9
           +TLLLVQ
Sbjct: 679 KTLLLVQ 685



>XP_005245677
          Length = 1173

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RTLLLVQ 9
           +TLLLVQ
Sbjct: 707 KTLLLVQ 713



>XP_006711697
          Length = 1179

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   RTLLLVQ 9
           +TLLLVQ
Sbjct: 716 KTLLLVQ 722



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.332    0.140    0.326 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 287,671
Number of extensions: 185
Number of successful extensions: 9
Number of sequences better than 10000.0: 9
Number of HSP's gapped: 9
Number of HSP's successfully gapped: 9
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)