Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

• Home Page
• Cancer-specific
• Epitope Search
• Data Retrieval
• Browse Gene
• Browse Tissue
• Sequence Similarity
• Partially Personalized
• Submit a Protein
• Vaccine Target
• Proteome Data
• NGS data
• BLAST & Predict
• Fully Personalized
• Proteins Pair
• Proteome Pair
• Advanced Tools
• Epitope Mapping
• Cross Reactivity
• Important Information
• Acknowledgement
• Algorithm
• Help
• Developers
• Contact

Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_853559                                                              17    3301
NP_001257348                                                           17    3301
XP_006715908                                                           17    3301
XP_006715907                                                           17    3301
XP_005250182                                                           17    3301
NP_005575                                                              16    5631
NP_000493                                                              16    7355
NP_065833                                                              16    7355
XP_006722191                                                           16    7355
NP_071375                                                              15    9605
XP_005255529                                                           15    9605
XP_005255527                                                           15    9605
XP_005255528                                                           15    9605
XP_005255185                                                           15    9605
NP_653275                                                              15    9605

>NP_853559
          Length = 317

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLKLRIRD 9
           VS+K+RI D
Sbjct: 155 VSIKIRIHD 163



>NP_001257348
          Length = 317

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLKLRIRD 9
           VS+K+RI D
Sbjct: 155 VSIKIRIHD 163



>XP_006715908
          Length = 237

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VSLKLRIRD 9
          VS+K+RI D
Sbjct: 75 VSIKIRIHD 83



>XP_006715907
          Length = 237

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VSLKLRIRD 9
          VS+K+RI D
Sbjct: 75 VSIKIRIHD 83



>XP_005250182
          Length = 317

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLKLRIRD 9
           VS+K+RI D
Sbjct: 155 VSIKIRIHD 163



>NP_005575
          Length = 359

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   LKLRIRD 9
           +KLRIRD
Sbjct: 158 VKLRIRD 164



>NP_000493
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLKLRIRD 9
           VSL LR+R+
Sbjct: 319 VSLSLRLRN 327



>NP_065833
          Length = 505

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLKLRIRD 9
           VSLK R+R+
Sbjct: 179 VSLKQRLRE 187



>XP_006722191
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLKLRIRD 9
           VSL LR+R+
Sbjct: 319 VSLSLRLRN 327



>NP_071375
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LKLRIRD 9
           L+LR+RD
Sbjct: 657 LRLRLRD 663



>XP_005255529
          Length = 557

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LKLRIRD 9
           L+LR+RD
Sbjct: 239 LRLRLRD 245



>XP_005255527
          Length = 859

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LKLRIRD 9
           L+LR+RD
Sbjct: 541 LRLRLRD 547



>XP_005255528
          Length = 1003

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LKLRIRD 9
           L+LR+RD
Sbjct: 685 LRLRLRD 691



>XP_005255185
          Length = 258

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLKLRIRD 9
           +LKLR+R+
Sbjct: 201 TLKLRLRE 208



>NP_653275
          Length = 485

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SLKLRIRD 9
           +LKLR+R+
Sbjct: 428 TLKLRLRE 435



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.334    0.150    0.373 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 358,598
Number of extensions: 447
Number of successful extensions: 15
Number of sequences better than 10000.0: 15
Number of HSP's gapped: 15
Number of HSP's successfully gapped: 15
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.2 bits)
S2: 28 (15.4 bits)