Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           20     509
NP_004561                                                              20     509
XP_005253450                                                           20     509
XP_006719151                                                           20     509
NP_003046                                                              17    3301
NP_001017420                                                           16    5631
XP_006716360                                                           16    5631
NP_004915                                                              16    7355
NP_001245300                                                           16    7355
NP_001095                                                              16    7355
NP_780775                                                              16    7355
NP_001230199                                                           16    7355
NP_001094                                                              16    7355
NP_001265272                                                           16    7355
NP_055176                                                              16    7355
NP_001093                                                              16    7355
NP_001123477                                                           16    7355
NP_001123476                                                           16    7355
NP_060862                                                              16    7355
XP_005259339                                                           16    7355
XP_005259338                                                           16    7355
XP_006723469                                                           16    7355
XP_005263172                                                           16    7355
NP_001073932                                                           15    9605
NP_001368                                                              15    9605
NP_057044                                                              15    9605
NP_115523                                                              15    9605
NP_001035862                                                           15    9605
NP_001265322                                                           15    9605
NP_065770                                                              15    9605
NP_001265043                                                           15    9605
NP_001265042                                                           15    9605
NP_001455                                                              15    9605
XP_006725182                                                           15    9605
XP_006722357                                                           15    9605
XP_005269230                                                           15    9605
XP_005269231                                                           15    9605
XP_005269229                                                           15    9605
XP_006718966                                                           15    9605
XP_005273525                                                           15    9605
>NP_001275701
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  VSLGFEQIV 9
          VSLGF+QIV
Sbjct: 36 VSLGFDQIV 44
>NP_004561
          Length = 1445

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  VSLGFEQIV 9
          VSLGF+QIV
Sbjct: 36 VSLGFDQIV 44
>XP_005253450
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  VSLGFEQIV 9
          VSLGF+QIV
Sbjct: 36 VSLGFDQIV 44
>XP_006719151
          Length = 1486

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  VSLGFEQIV 9
          VSLGF+QIV
Sbjct: 36 VSLGFDQIV 44
>NP_003046
          Length = 532

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SLGFEQI 8
           SLGFEQ+
Sbjct: 445 SLGFEQL 451
>NP_001017420
          Length = 601

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LGFEQIV 9
           LGF+Q+V
Sbjct: 463 LGFQQVV 469
>XP_006716360
          Length = 601

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   LGFEQIV 9
           LGF+Q+V
Sbjct: 463 LGFQQVV 469
>NP_004915
          Length = 911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 127 VSIGAEEIV 135
>NP_001245300
          Length = 944

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 165 VSIGAEEIV 173
>NP_001095
          Length = 901

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 122 VSIGAEEIV 130
>NP_780775
          Length = 1026

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VSLG+E ++
Sbjct: 916 VSLGWEPVI 924
>NP_001230199
          Length = 1100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VSLG+E ++
Sbjct: 990 VSLGWEPVI 998
>NP_001094
          Length = 894

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 115 VSIGAEEIV 123
>NP_001265272
          Length = 894

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 115 VSIGAEEIV 123
>NP_055176
          Length = 1100

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VSLG+E ++
Sbjct: 990 VSLGWEPVI 998
>NP_001093
          Length = 892

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 108 VSIGAEEIV 116
>NP_001123477
          Length = 887

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 108 VSIGAEEIV 116
>NP_001123476
          Length = 914

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 108 VSIGAEEIV 116
>NP_060862
          Length = 2104

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VSLGFEQIV 9
            +SLGFE  +
Sbjct: 1709 LSLGFENFI 1717
>XP_005259339
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 127 VSIGAEEIV 135
>XP_005259338
          Length = 906

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 127 VSIGAEEIV 135
>XP_006723469
          Length = 911

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           VS+G E+IV
Sbjct: 127 VSIGAEEIV 135
>XP_005263172
          Length = 2104

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 7/9 (77%)

Query: 1    VSLGFEQIV 9
            +SLGFE  +
Sbjct: 1709 LSLGFENFI 1717
>NP_001073932
          Length = 4314

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SLGFEQIV 9
           +L FEQI+
Sbjct: 643 ALAFEQII 650
>NP_001368
          Length = 4307

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SLGFEQIV 9
           +L FEQI+
Sbjct: 643 ALAFEQII 650
>NP_057044
          Length = 273

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 9/9 (100%)

Query: 1   VSLGFEQIV 9
           ++LGFE+++
Sbjct: 162 LTLGFERVL 170
>NP_115523
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           V +GFE+I+
Sbjct: 590 VHVGFEEIL 598
>NP_001035862
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           V +GFE+I+
Sbjct: 590 VHVGFEEIL 598
>NP_001265322
          Length = 712

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           V +GFE+I+
Sbjct: 590 VHVGFEEIL 598
>NP_065770
          Length = 2036

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 2   SLGFEQI 8
           SLGFE++
Sbjct: 202 SLGFERL 208
>NP_001265043
          Length = 672

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLGFEQIV 9
           S+GFE ++
Sbjct: 133 SVGFEHVI 140
>NP_001265042
          Length = 716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLGFEQIV 9
           S+GFE ++
Sbjct: 133 SVGFEHVI 140
>NP_001455
          Length = 735

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLGFEQIV 9
           S+GFE ++
Sbjct: 133 SVGFEHVI 140
>XP_006725182
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLGFEQIV 9
           S+GFE ++
Sbjct: 133 SVGFEHVI 140
>XP_006722357
          Length = 347

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 6/7 (85%)

Query: 1   VSLGFEQ 7
           VSLGF Q
Sbjct: 182 VSLGFPQ 188
>XP_005269230
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           V +GFE+I+
Sbjct: 621 VHVGFEEIL 629
>XP_005269231
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           V +GFE+I+
Sbjct: 621 VHVGFEEIL 629
>XP_005269229
          Length = 743

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VSLGFEQIV 9
           V +GFE+I+
Sbjct: 621 VHVGFEEIL 629
>XP_006718966
          Length = 4300

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   SLGFEQIV 9
           +L FEQI+
Sbjct: 643 ALAFEQII 650
>XP_005273525
          Length = 705

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 2   SLGFEQIV 9
           S+GFE ++
Sbjct: 133 SVGFEHVI 140
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.327    0.147    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 482,585
Number of extensions: 505
Number of successful extensions: 40
Number of sequences better than 10000.0: 40
Number of HSP's gapped: 40
Number of HSP's successfully gapped: 40
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.0 bits)
S2: 28 (15.4 bits)

CANCERTOPE    |     Raghava's Group    |     IMTECH    |     CSIR    |     CRDD    |     GPSR Package