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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_036284                                                              18    1935
XP_006710605                                                           18    1935
XP_005250395                                                           16    5631
NP_775926                                                              16    7355
NP_001265229                                                           16    7355
NP_078966                                                              16    7355
NP_001096619                                                           16    7355
NP_115618                                                              16    7355
XP_006723004                                                           16    7355
XP_006713726                                                           16    7355
NP_060685                                                              16    7355
NP_112165                                                              15    9605
NP_000252                                                              15    9605
NP_115272                                                              15    9605
NP_061750                                                              15    9605
NP_001243783                                                           15    9605
NP_001243823                                                           15    9605
NP_001229260                                                           15    9605
NP_001229259                                                           15    9605
NP_001243788                                                           15    9605
NP_001243789                                                           15    9605
NP_001229256                                                           15    9605
NP_001229255                                                           15    9605
NP_001243781                                                           15    9605
NP_001229257                                                           15    9605
NP_001229258                                                           15    9605
NP_001229261                                                           15    9605
NP_001243796                                                           15    9605
NP_001243784                                                           15    9605
NP_001243786                                                           15    9605
NP_001243792                                                           15    9605
NP_001243790                                                           15    9605
XP_006710304                                                           15    9605
NP_958804                                                              15    9605
NP_115268                                                              15    9605
NP_061747                                                              15    9605

>NP_036284
          Length = 353

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   VSKTLTLN 8
           VSKTLT+N
Sbjct: 93  VSKTLTVN 100



>XP_006710605
          Length = 353

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 1   VSKTLTLN 8
           VSKTLT+N
Sbjct: 93  VSKTLTVN 100



>XP_005250395
          Length = 110

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 2  SKTLTLNL 9
          +KTL+LNL
Sbjct: 68 AKTLSLNL 75



>NP_775926
          Length = 334

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KTLTLNL 9
           +TLTLNL
Sbjct: 324 QTLTLNL 330



>NP_001265229
          Length = 398

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 3   KTLTLNL 9
           +TLTLNL
Sbjct: 388 QTLTLNL 394



>NP_078966
          Length = 14507

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3     KTLTLN 8
             KTLTLN
Sbjct: 14075 KTLTLN 14080



>NP_001096619
          Length = 378

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1 VSKTLTLNL 9
         +SKT+ +NL
Sbjct: 1 MSKTMAMNL 9



>NP_115618
          Length = 1896

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    KTLTLN 8
            KTLTLN
Sbjct: 1501 KTLTLN 1506



>XP_006723004
          Length = 6443

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    KTLTLN 8
            KTLTLN
Sbjct: 6011 KTLTLN 6016



>XP_006713726
          Length = 1910

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 3    KTLTLN 8
            KTLTLN
Sbjct: 1519 KTLTLN 1524



>NP_060685
          Length = 439

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1 VSKTLTLNL 9
         +SKT+ +NL
Sbjct: 1 MSKTMAMNL 9



>NP_112165
          Length = 320

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   TLTLNL 9
           TLTLNL
Sbjct: 161 TLTLNL 166



>NP_000252
          Length = 504

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VSKTLTL 7
           +SKTLT+
Sbjct: 459 ISKTLTI 465



>NP_115272
          Length = 808

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKTLTLNL 9
           SKT+TL++
Sbjct: 435 SKTITLHI 442



>NP_061750
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKTLTLNL 9
           SKT+TL++
Sbjct: 435 SKTITLHI 442



>NP_001243783
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001243823
          Length = 559

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001229260
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001229259
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001243788
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001243789
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001229256
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001229255
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001243781
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001229257
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001229258
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001229261
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001243796
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001243784
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001243786
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001243792
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_001243790
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>XP_006710304
          Length = 311

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2  SKTLTLN 8
          SKTLTL+
Sbjct: 45 SKTLTLH 51



>NP_958804
          Length = 530

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/7 (85%), Positives = 7/7 (100%)

Query: 2   SKTLTLN 8
           SKTLTL+
Sbjct: 264 SKTLTLH 270



>NP_115268
          Length = 814

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKTLTLNL 9
           SKT+TL++
Sbjct: 435 SKTITLHI 442



>NP_061747
          Length = 929

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   SKTLTLNL 9
           SKT+TL++
Sbjct: 435 SKTITLHI 442



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.311    0.124    0.293 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 297,752
Number of extensions: 317
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.6 bits)
S2: 28 (15.4 bits)