Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005091                                                              18    1135
NP_653080                                                              18    1135
XP_005267292                                                           18    1135
XP_005267291                                                           18    1135
XP_005267290                                                           18    1135
NP_001019765                                                           17    2528
NP_001136156                                                           17    3301
XP_005266652                                                           17    3301
XP_005266651                                                           17    3301
XP_006719957                                                           17    3301
XP_005266650                                                           17    3301
NP_005549                                                              16    5631
NP_079471                                                              15    9605
NP_001269914                                                           15    9605
NP_056234                                                              15    9605
NP_001129225                                                           15    9605
NP_001129224                                                           15    9605
NP_009196                                                              15    9605
NP_060932                                                              15    9605
XP_005274542                                                           15    9605
XP_006716560                                                           15    9605
XP_006716559                                                           15    9605
NP_001131032                                                           15    9605
>NP_005091
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SEKLTASE 9
           SEKLTASE
Sbjct: 347 SEKLTASE 354
>NP_653080
          Length = 1684

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SEKLTASE 9
           SEKLTASE
Sbjct: 249 SEKLTASE 256
>XP_005267292
          Length = 1677

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SEKLTASE 9
           SEKLTASE
Sbjct: 242 SEKLTASE 249
>XP_005267291
          Length = 1684

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SEKLTASE 9
           SEKLTASE
Sbjct: 249 SEKLTASE 256
>XP_005267290
          Length = 1782

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2   SEKLTASE 9
           SEKLTASE
Sbjct: 347 SEKLTASE 354
>NP_001019765
          Length = 145

 Score = 17.3 bits (33), Expect =  2528
 Identities = 7/9 (77%), Positives = 9/9 (100%)

Query: 1  VSEKLTASE 9
          VSE+LTA+E
Sbjct: 68 VSEELTAAE 76
>NP_001136156
          Length = 669

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SEKLTASE 9
           S+KLTASE
Sbjct: 423 SKKLTASE 430
>XP_005266652
          Length = 487

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SEKLTASE 9
           S+KLTASE
Sbjct: 241 SKKLTASE 248
>XP_005266651
          Length = 669

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SEKLTASE 9
           S+KLTASE
Sbjct: 423 SKKLTASE 430
>XP_006719957
          Length = 669

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SEKLTASE 9
           S+KLTASE
Sbjct: 423 SKKLTASE 430
>XP_005266650
          Length = 669

 Score = 16.9 bits (32), Expect =  3301
 Identities = 7/8 (87%), Positives = 8/8 (100%)

Query: 2   SEKLTASE 9
           S+KLTASE
Sbjct: 423 SKKLTASE 430
>NP_005549
          Length = 517

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSEKLTASE 9
           +SEK+ ASE
Sbjct: 202 ISEKVLASE 210
>NP_079471
          Length = 372

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SEKLTASE 9
           SE+LTA E
Sbjct: 324 SERLTAPE 331
>NP_001269914
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SEKLTASE 9
           SE+LTA E
Sbjct: 158 SERLTAPE 165
>NP_056234
          Length = 2828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    VSEKLTA 7
            +SEK+TA
Sbjct: 2548 ISEKITA 2554
>NP_001129225
          Length = 601

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SEKLTASE 9
           SEK+ ASE
Sbjct: 178 SEKILASE 185
>NP_001129224
          Length = 677

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SEKLTASE 9
           SEK+ ASE
Sbjct: 178 SEKILASE 185
>NP_009196
          Length = 644

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SEKLTASE 9
           SEK+ ASE
Sbjct: 178 SEKILASE 185
>NP_060932
          Length = 75

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1  VSEKLTASE 9
          ++EKLTA E
Sbjct: 50 LNEKLTALE 58
>XP_005274542
          Length = 2853

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1    VSEKLTA 7
            +SEK+TA
Sbjct: 2573 ISEKITA 2579
>XP_006716560
          Length = 206

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SEKLTASE 9
           SE+LTA E
Sbjct: 158 SERLTAPE 165
>XP_006716559
          Length = 262

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   SEKLTASE 9
           SE+LTA E
Sbjct: 214 SERLTAPE 221
>NP_001131032
          Length = 566

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 8/8 (100%)

Query: 1  VSEKLTAS 8
          VSE+LTA+
Sbjct: 24 VSEELTAA 31
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.280   0.0987    0.206 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 327,610
Number of extensions: 191
Number of successful extensions: 23
Number of sequences better than 10000.0: 23
Number of HSP's gapped: 23
Number of HSP's successfully gapped: 23
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 18 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (14.7 bits)
S2: 28 (15.4 bits)

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