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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000236                                                              17    2528
NP_001120972                                                           17    2528
XP_006716054                                                           17    2528
XP_006716053                                                           17    2528
XP_006716052                                                           17    2528
XP_006716051                                                           17    2528
NP_689957                                                              16    5631
XP_005247977                                                           16    7355
NP_055594                                                              16    7355
XP_006719294                                                           15    9605
NP_001138947                                                           15    9605

>NP_000236
          Length = 1390

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSARSVSST 9
           VSARSVS T
Sbjct: 984 VSARSVSPT 992



>NP_001120972
          Length = 1408

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VSARSVSST 9
            VSARSVS T
Sbjct: 1002 VSARSVSPT 1010



>XP_006716054
          Length = 960

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSARSVSST 9
           VSARSVS T
Sbjct: 554 VSARSVSPT 562



>XP_006716053
          Length = 960

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VSARSVSST 9
           VSARSVS T
Sbjct: 554 VSARSVSPT 562



>XP_006716052
          Length = 1409

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VSARSVSST 9
            VSARSVS T
Sbjct: 1003 VSARSVSPT 1011



>XP_006716051
          Length = 1427

 Score = 17.3 bits (33), Expect =  2528
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1    VSARSVSST 9
            VSARSVS T
Sbjct: 1021 VSARSVSPT 1029



>NP_689957
          Length = 2213

 Score = 16.2 bits (30), Expect =  5631
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1    VSARSVSST 9
            VSA +VSST
Sbjct: 1283 VSAEAVSST 1291



>XP_005247977
          Length = 756

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   VSARSVS 7
           VSARSVS
Sbjct: 108 VSARSVS 114



>NP_055594
          Length = 780

 Score = 15.8 bits (29), Expect =  7355
 Identities = 7/7 (100%), Positives = 7/7 (100%)

Query: 1   VSARSVS 7
           VSARSVS
Sbjct: 108 VSARSVS 114



>XP_006719294
          Length = 2348

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSARSVSST 9
           +SA+S SST
Sbjct: 769 ISAKSESST 777



>NP_001138947
          Length = 2351

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/9 (66%), Positives = 8/9 (88%)

Query: 1   VSARSVSST 9
           +SA+S SST
Sbjct: 769 ISAKSESST 777



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.287   0.0918    0.186 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 174,424
Number of extensions: 101
Number of successful extensions: 11
Number of sequences better than 10000.0: 11
Number of HSP's gapped: 11
Number of HSP's successfully gapped: 11
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 17 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.1 bits)
S2: 28 (15.4 bits)