Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_005742                                                              19     665
NP_671714                                                              19     665
XP_006715890                                                           19     665
XP_005250160                                                           19     665
XP_005250159                                                           19     665
NP_004167                                                              16    5631
NP_004590                                                              16    5631
NP_001005291                                                           16    5631
XP_006724373                                                           16    5631
XP_006721633                                                           16    5631
XP_005256829                                                           16    5631
NP_009090                                                              15    9605
NP_001138766                                                           15    9605
NP_001257545                                                           15    9605
NP_060767                                                              15    9605
NP_001138765                                                           15    9605
NP_002754                                                              15    9605
NP_003038                                                              15    9605
NP_783863                                                              15    9605
NP_944489                                                              15    9605
NP_114102                                                              15    9605
XP_005259182                                                           15    9605
XP_006719749                                                           15    9605
XP_005269275                                                           15    9605
XP_005265529                                                           15    9605
XP_006713389                                                           15    9605
XP_005265528                                                           15    9605
XP_005265527                                                           15    9605
XP_005265525                                                           15    9605
XP_005265524                                                           15    9605
XP_005265523                                                           15    9605
XP_005265522                                                           15    9605
XP_005265521                                                           15    9605
XP_005265520                                                           15    9605
XP_005265519                                                           15    9605
XP_005265518                                                           15    9605
XP_005265517                                                           15    9605
XP_005265516                                                           15    9605
XP_005265515                                                           15    9605
XP_005265514                                                           15    9605
XP_005265513                                                           15    9605
XP_005265373                                                           15    9605
XP_006711518                                                           15    9605
XP_005273252                                                           15    9605
XP_005273253                                                           15    9605
XP_005273251                                                           15    9605
NP_062538                                                              15    9605
>NP_005742
          Length = 3907

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    VRQSINDEI 9
            VRQSI+DEI
Sbjct: 1561 VRQSIHDEI 1569
>NP_671714
          Length = 3899

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    VRQSINDEI 9
            VRQSI+DEI
Sbjct: 1561 VRQSIHDEI 1569
>XP_006715890
          Length = 3860

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    VRQSINDEI 9
            VRQSI+DEI
Sbjct: 1561 VRQSIHDEI 1569
>XP_005250160
          Length = 3939

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    VRQSINDEI 9
            VRQSI+DEI
Sbjct: 1561 VRQSIHDEI 1569
>XP_005250159
          Length = 3952

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1    VRQSINDEI 9
            VRQSI+DEI
Sbjct: 1561 VRQSIHDEI 1569
>NP_004167
          Length = 1147

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           R SIND+I
Sbjct: 336 RSSINDKI 343
>NP_004590
          Length = 1141

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           R SIND+I
Sbjct: 343 RSSINDKI 350
>NP_001005291
          Length = 1177

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           R SIND+I
Sbjct: 366 RSSINDKI 373
>XP_006724373
          Length = 1111

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           R SIND+I
Sbjct: 313 RSSINDKI 320
>XP_006721633
          Length = 1123

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           R SIND+I
Sbjct: 312 RSSINDKI 319
>XP_005256829
          Length = 1145

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           R SIND+I
Sbjct: 334 RSSINDKI 341
>NP_009090
          Length = 811

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 758 KQSITDEL 765
>NP_001138766
          Length = 612

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RQSIND 7
           RQS+ND
Sbjct: 592 RQSLND 597
>NP_001257545
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           RQ+IND +
Sbjct: 640 RQAINDGV 647
>NP_060767
          Length = 654

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RQSIND 7
           RQS+ND
Sbjct: 634 RQSLND 639
>NP_001138765
          Length = 636

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RQSIND 7
           RQS+ND
Sbjct: 616 RQSLND 621
>NP_002754
          Length = 737

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           RQ+IND +
Sbjct: 640 RQAINDGV 647
>NP_003038
          Length = 815

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RQSINDEI 9
           ++SIN+EI
Sbjct: 515 KRSINEEI 522
>NP_783863
          Length = 1027

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRQSINDE 8
           VR+ +NDE
Sbjct: 125 VREFLNDE 132
>NP_944489
          Length = 976

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRQSINDE 8
           VR+ +NDE
Sbjct: 125 VREFLNDE 132
>NP_114102
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SINDEI 9
           SINDE+
Sbjct: 155 SINDEV 160
>XP_005259182
          Length = 275

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 4   SINDEI 9
           SINDE+
Sbjct: 155 SINDEV 160
>XP_006719749
          Length = 977

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRQSINDE 8
           VR+ +NDE
Sbjct: 125 VREFLNDE 132
>XP_005269275
          Length = 1028

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRQSINDE 8
           VR+ +NDE
Sbjct: 125 VREFLNDE 132
>XP_005265529
          Length = 768

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 715 KQSITDEL 722
>XP_006713389
          Length = 770

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 717 KQSITDEL 724
>XP_005265528
          Length = 790

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 737 KQSITDEL 744
>XP_005265527
          Length = 792

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 739 KQSITDEL 746
>XP_005265525
          Length = 806

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 753 KQSITDEL 760
>XP_005265524
          Length = 809

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 756 KQSITDEL 763
>XP_005265523
          Length = 811

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 758 KQSITDEL 765
>XP_005265522
          Length = 813

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 760 KQSITDEL 767
>XP_005265521
          Length = 821

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 771 KQSITDEL 778
>XP_005265520
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 771 KQSITDEL 778
>XP_005265519
          Length = 826

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 773 KQSITDEL 780
>XP_005265518
          Length = 828

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 775 KQSITDEL 782
>XP_005265517
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 777 KQSITDEL 784
>XP_005265516
          Length = 830

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 777 KQSITDEL 784
>XP_005265515
          Length = 844

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 794 KQSITDEL 801
>XP_005265514
          Length = 845

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 792 KQSITDEL 799
>XP_005265513
          Length = 847

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           +QSI DE+
Sbjct: 794 KQSITDEL 801
>XP_005265373
          Length = 599

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 2   RQSIND 7
           RQS+ND
Sbjct: 579 RQSLND 584
>XP_006711518
          Length = 680

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           RQ+IND +
Sbjct: 640 RQAINDGV 647
>XP_005273252
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           RQ+IND +
Sbjct: 640 RQAINDGV 647
>XP_005273253
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           RQ+IND +
Sbjct: 640 RQAINDGV 647
>XP_005273251
          Length = 738

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RQSINDEI 9
           RQ+IND +
Sbjct: 640 RQAINDGV 647
>NP_062538
          Length = 975

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RQSINDEI 9
          RQ+I DE+
Sbjct: 65 RQAIEDEL 72
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.313    0.132    0.323 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 469,538
Number of extensions: 735
Number of successful extensions: 47
Number of sequences better than 10000.0: 47
Number of HSP's gapped: 47
Number of HSP's successfully gapped: 47
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.5 bits)
S2: 28 (15.4 bits)

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