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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_733751                                                              20     509
XP_006716142                                                           20     509
XP_005250088                                                           20     509
XP_006716141                                                           20     509
XP_005250085                                                           20     509
XP_006716140                                                           20     509
XP_005250084                                                           20     509
XP_005250083                                                           20     509
XP_005250082                                                           20     509
NP_055986                                                              18    1935
NP_001257869                                                           18    1935
XP_005255257                                                           18    1935
XP_005255256                                                           18    1935
XP_005255255                                                           18    1935
XP_005255254                                                           18    1935
XP_005255252                                                           18    1935
NP_079106                                                              17    3301
XP_005248430                                                           17    3301
NP_001004755                                                           16    5631
NP_001191339                                                           16    7355
NP_002994                                                              16    7355
NP_001006624                                                           16    7355
NP_060853                                                              16    7355
NP_001006623                                                           16    7355
NP_001137473                                                           16    7355
NP_001137471                                                           16    7355
NP_001137470                                                           16    7355
NP_998821                                                              16    7355
NP_001191337                                                           16    7355
NP_001034662                                                           16    7355
XP_005263754                                                           16    7355
NP_001274273                                                           15    9605
NP_000114                                                              15    9605
NP_000052                                                              15    9605
NP_001001670                                                           15    9605
NP_001191354                                                           15    9605

>NP_733751
          Length = 4911

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RPVFVIRI 9
            RPVFVIRI
Sbjct: 4608 RPVFVIRI 4615



>XP_006716142
          Length = 4927

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RPVFVIRI 9
            RPVFVIRI
Sbjct: 4624 RPVFVIRI 4631



>XP_005250088
          Length = 4928

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RPVFVIRI 9
            RPVFVIRI
Sbjct: 4625 RPVFVIRI 4632



>XP_006716141
          Length = 4959

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RPVFVIRI 9
            RPVFVIRI
Sbjct: 4656 RPVFVIRI 4663



>XP_005250085
          Length = 4979

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RPVFVIRI 9
            RPVFVIRI
Sbjct: 4680 RPVFVIRI 4687



>XP_006716140
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RPVFVIRI 9
            RPVFVIRI
Sbjct: 4679 RPVFVIRI 4686



>XP_005250084
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RPVFVIRI 9
            RPVFVIRI
Sbjct: 4679 RPVFVIRI 4686



>XP_005250083
          Length = 4982

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RPVFVIRI 9
            RPVFVIRI
Sbjct: 4679 RPVFVIRI 4686



>XP_005250082
          Length = 4983

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/8 (100%), Positives = 8/8 (100%)

Query: 2    RPVFVIRI 9
            RPVFVIRI
Sbjct: 4680 RPVFVIRI 4687



>NP_055986
          Length = 1125

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VRPVFVIRI 9
           VRPVF+I I
Sbjct: 688 VRPVFLISI 696



>NP_001257869
          Length = 1111

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VRPVFVIRI 9
           VRPVF+I I
Sbjct: 674 VRPVFLISI 682



>XP_005255257
          Length = 774

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VRPVFVIRI 9
           VRPVF+I I
Sbjct: 337 VRPVFLISI 345



>XP_005255256
          Length = 780

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VRPVFVIRI 9
           VRPVF+I I
Sbjct: 343 VRPVFLISI 351



>XP_005255255
          Length = 951

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VRPVFVIRI 9
           VRPVF+I I
Sbjct: 514 VRPVFLISI 522



>XP_005255254
          Length = 1051

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VRPVFVIRI 9
           VRPVF+I I
Sbjct: 614 VRPVFLISI 622



>XP_005255252
          Length = 1124

 Score = 17.7 bits (34), Expect =  1935
 Identities = 7/9 (77%), Positives = 8/9 (88%)

Query: 1   VRPVFVIRI 9
           VRPVF+I I
Sbjct: 687 VRPVFLISI 695



>NP_079106
          Length = 534

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRPVFVIR 8
           +RPVFV R
Sbjct: 170 IRPVFVSR 177



>XP_005248430
          Length = 486

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRPVFVIR 8
           +RPVFV R
Sbjct: 122 IRPVFVSR 129



>NP_001004755
          Length = 315

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/8 (50%), Positives = 7/8 (87%)

Query: 1  VRPVFVIR 8
          V P+F++R
Sbjct: 10 VEPIFILR 17



>NP_001191339
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RPVFVIRI 9
           RP++V+R+
Sbjct: 339 RPLYVLRL 346



>NP_002994
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RPVFVIRI 9
           RP++V+R+
Sbjct: 339 RPLYVLRL 346



>NP_001006624
          Length = 257

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   PVFVIR 8
           PVFV+R
Sbjct: 121 PVFVVR 126



>NP_060853
          Length = 1336

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   PVFVIR 8
           PVFV+R
Sbjct: 121 PVFVVR 126



>NP_001006623
          Length = 326

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   PVFVIR 8
           PVFV+R
Sbjct: 121 PVFVVR 126



>NP_001137473
          Length = 681

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RPVFVIRI 9
           RP++V+R+
Sbjct: 305 RPLYVLRL 312



>NP_001137471
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RPVFVIRI 9
           RP++V+R+
Sbjct: 339 RPLYVLRL 346



>NP_001137470
          Length = 715

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RPVFVIRI 9
           RP++V+R+
Sbjct: 339 RPLYVLRL 346



>NP_998821
          Length = 491

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRPVFVIR 8
           V+P F+IR
Sbjct: 480 VQPCFIIR 487



>NP_001191337
          Length = 719

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RPVFVIRI 9
           RP++V+R+
Sbjct: 339 RPLYVLRL 346



>NP_001034662
          Length = 719

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 2   RPVFVIRI 9
           RP++V+R+
Sbjct: 339 RPLYVLRL 346



>XP_005263754
          Length = 1133

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   PVFVIR 8
           PVFV+R
Sbjct: 121 PVFVVR 126



>NP_001274273
          Length = 693

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   RPVFVI 7
           RP+F+I
Sbjct: 502 RPIFII 507



>NP_000114
          Length = 1186

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1  VRPVFV 6
          +RP+FV
Sbjct: 70 IRPIFV 75



>NP_000052
          Length = 659

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 2   RPVFVI 7
           RP+F+I
Sbjct: 468 RPIFII 473



>NP_001001670
          Length = 1576

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   PVFVIRI 9
           P+F+IRI
Sbjct: 595 PLFLIRI 601



>NP_001191354
          Length = 1640

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 1   VRPVFV 6
           +RP+FV
Sbjct: 524 IRPIFV 529



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.355    0.169    0.507 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 489,751
Number of extensions: 701
Number of successful extensions: 36
Number of sequences better than 10000.0: 36
Number of HSP's gapped: 36
Number of HSP's successfully gapped: 36
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 14 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.9 bits)
S2: 28 (15.4 bits)