Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_008835                                                              19     665
NP_001075109                                                           19     665
NP_064544                                                              17    2528
NP_001241878                                                           17    2528
NP_006234                                                              17    4312
NP_001186685                                                           17    4312
NP_001155197                                                           17    4312
NP_848702                                                              17    4312
NP_001155198                                                           17    4312
NP_848701                                                              17    4312
NP_002710                                                              17    4312
NP_001269109                                                           17    4312
NP_006237                                                              17    4312
NP_001269111                                                           17    4312
NP_001269110                                                           17    4312
NP_001269108                                                           17    4312
XP_005267884                                                           17    4312
XP_005267883                                                           17    4312
XP_005267881                                                           17    4312
XP_005267880                                                           17    4312
XP_005267879                                                           17    4312
XP_005267877                                                           17    4312
XP_005267876                                                           17    4312
NP_060587                                                              16    5631
XP_005270020                                                           16    5631
XP_005270019                                                           16    5631
NP_001275964                                                           16    7355
NP_001275963                                                           16    7355
NP_612352                                                              16    7355
NP_958930                                                              16    7355
NP_958929                                                              16    7355
NP_958931                                                              16    7355
NP_001121902                                                           15    9605
NP_001121901                                                           15    9605
NP_001012977                                                           15    9605
NP_001012975                                                           15    9605
NP_001158016                                                           15    9605
NP_001158014                                                           15    9605
NP_001158013                                                           15    9605
NP_001158012                                                           15    9605
NP_001158011                                                           15    9605
NP_001158010                                                           15    9605
NP_001001850                                                           15    9605
NP_061132                                                              15    9605
NP_001158009                                                           15    9605
XP_006721093                                                           15    9605
XP_006721091                                                           15    9605
XP_006714339                                                           15    9605
XP_006714338                                                           15    9605
XP_005247513                                                           15    9605
XP_005247512                                                           15    9605
>NP_008835
          Length = 4128

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  VRKSLNNIE 9
          VRKSLN+IE
Sbjct: 69 VRKSLNSIE 77
>NP_001075109
          Length = 4097

 Score = 19.2 bits (38), Expect =   665
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1  VRKSLNNIE 9
          VRKSLN+IE
Sbjct: 69 VRKSLNSIE 77
>NP_064544
          Length = 1026

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VRKSLNNIE 9
           ++KS+NN+E
Sbjct: 758 LKKSINNLE 766
>NP_001241878
          Length = 596

 Score = 17.3 bits (33), Expect =  2528
 Identities = 5/9 (55%), Positives = 9/9 (100%)

Query: 1   VRKSLNNIE 9
           ++KS+NN+E
Sbjct: 328 LKKSINNLE 336
>NP_006234
          Length = 486

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 225 IRKQINNI 232
>NP_001186685
          Length = 429

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 168 IRKQINNI 175
>NP_001155197
          Length = 555

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 231 IRKQINNI 238
>NP_848702
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 200 IRKQINNI 207
>NP_001155198
          Length = 540

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 255 IRKQINNI 262
>NP_848701
          Length = 485

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 200 IRKQINNI 207
>NP_002710
          Length = 524

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 200 IRKQINNI 207
>NP_001269109
          Length = 462

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 217 IRKQINNI 224
>NP_006237
          Length = 467

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 217 IRKQINNI 224
>NP_001269111
          Length = 391

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 141 IRKQINNI 148
>NP_001269110
          Length = 391

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 141 IRKQINNI 148
>NP_001269108
          Length = 467

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 217 IRKQINNI 224
>XP_005267884
          Length = 449

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 125 IRKQINNI 132
>XP_005267883
          Length = 500

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 176 IRKQINNI 183
>XP_005267881
          Length = 539

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 260 IRKQINNI 267
>XP_005267880
          Length = 545

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 260 IRKQINNI 267
>XP_005267879
          Length = 556

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 260 IRKQINNI 267
>XP_005267877
          Length = 579

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 255 IRKQINNI 262
>XP_005267876
          Length = 584

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           +RK +NNI
Sbjct: 260 IRKQINNI 267
>NP_060587
          Length = 1224

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VRKSLNNI 8
           VR+S NNI
Sbjct: 316 VRRSYNNI 323
>XP_005270020
          Length = 965

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VRKSLNNI 8
          VR+S NNI
Sbjct: 57 VRRSYNNI 64
>XP_005270019
          Length = 965

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1  VRKSLNNI 8
          VR+S NNI
Sbjct: 57 VRRSYNNI 64
>NP_001275964
          Length = 542

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VRKSLNNIE 9
          +R+S+ NIE
Sbjct: 71 LRRSMENIE 79
>NP_001275963
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VRKSLNNIE 9
          +R+S+ NIE
Sbjct: 71 LRRSMENIE 79
>NP_612352
          Length = 315

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VRKSLNNIE 9
          +R+S+ NIE
Sbjct: 71 LRRSMENIE 79
>NP_958930
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VRKSLNNIE 9
          +R+S+ NIE
Sbjct: 71 LRRSMENIE 79
>NP_958929
          Length = 619

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VRKSLNNIE 9
          +R+S+ NIE
Sbjct: 71 LRRSMENIE 79
>NP_958931
          Length = 637

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1  VRKSLNNIE 9
          +R+S+ NIE
Sbjct: 89 LRRSMENIE 97
>NP_001121902
          Length = 1028

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VRKSLNNI 8
           ++KS+NN+
Sbjct: 758 LKKSINNL 765
>NP_001121901
          Length = 1028

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VRKSLNNI 8
           ++KS+NN+
Sbjct: 758 LKKSINNL 765
>NP_001012977
          Length = 678

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRKSLNNIE 9
           +RK +N+IE
Sbjct: 574 LRKKVNDIE 582
>NP_001012975
          Length = 832

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRKSLNNIE 9
           +RK +N+IE
Sbjct: 574 LRKKVNDIE 582
>NP_001158016
          Length = 662

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRKSLNNIE 9
           +RK +N+IE
Sbjct: 574 LRKKVNDIE 582
>NP_001158014
          Length = 681

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRKSLNNIE 9
           +RK +N+IE
Sbjct: 574 LRKKVNDIE 582
>NP_001158013
          Length = 695

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRKSLNNIE 9
           +RK +N+IE
Sbjct: 574 LRKKVNDIE 582
>NP_001158012
          Length = 732

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRKSLNNIE 9
           +RK +N+IE
Sbjct: 452 LRKKVNDIE 460
>NP_001158011
          Length = 755

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRKSLNNIE 9
           +RK +N+IE
Sbjct: 574 LRKKVNDIE 582
>NP_001158010
          Length = 803

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRKSLNNIE 9
           +RK +N+IE
Sbjct: 574 LRKKVNDIE 582
>NP_001001850
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSLNNIE 9
           +S+NNIE
Sbjct: 245 ESINNIE 251
>NP_061132
          Length = 854

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRKSLNNIE 9
           +RK +N+IE
Sbjct: 574 LRKKVNDIE 582
>NP_001158009
          Length = 886

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRKSLNNIE 9
           +RK +N+IE
Sbjct: 606 LRKKVNDIE 614
>XP_006721093
          Length = 220

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RKSLNNIE 9
           RKS+N+I+
Sbjct: 207 RKSINSIK 214
>XP_006721091
          Length = 280

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 8/8 (100%)

Query: 2   RKSLNNIE 9
           RKS+N+I+
Sbjct: 267 RKSINSIK 274
>XP_006714339
          Length = 795

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VRKSLNNI 8
           ++KS+NN+
Sbjct: 525 LKKSINNL 532
>XP_006714338
          Length = 998

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VRKSLNNI 8
           ++KS+NN+
Sbjct: 728 LKKSINNL 735
>XP_005247513
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSLNNIE 9
           +S+NNIE
Sbjct: 245 ESINNIE 251
>XP_005247512
          Length = 294

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 3   KSLNNIE 9
           +S+NNIE
Sbjct: 245 ESINNIE 251
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.307    0.127    0.305 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 413,292
Number of extensions: 667
Number of successful extensions: 51
Number of sequences better than 10000.0: 51
Number of HSP's gapped: 51
Number of HSP's successfully gapped: 51
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.4 bits)
S2: 28 (15.4 bits)

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