Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              20     509
NP_444254                                                              20     509
NP_444253                                                              20     509
NP_444255                                                              20     509
XP_005247551                                                           20     509
XP_005247550                                                           20     509
XP_006713712                                                           20     509
XP_005247549                                                           20     509
NP_055921                                                              16    5631
XP_006713125                                                           16    5631
XP_006713124                                                           16    5631
NP_001242904                                                           16    7355
NP_001008496                                                           16    7355
NP_005742                                                              15    9605
NP_671714                                                              15    9605
NP_056477                                                              15    9605
NP_001268451                                                           15    9605
NP_001191055                                                           15    9605
NP_001268449                                                           15    9605
NP_006143                                                              15    9605
NP_001191056                                                           15    9605
NP_877954                                                              15    9605
NP_001268450                                                           15    9605
NP_115874                                                              15    9605
NP_003557                                                              15    9605
XP_005260916                                                           15    9605
XP_006719694                                                           15    9605
XP_005253431                                                           15    9605
XP_006719140                                                           15    9605
XP_006719139                                                           15    9605
XP_005253429                                                           15    9605
XP_006719138                                                           15    9605
XP_005253428                                                           15    9605
XP_005253427                                                           15    9605
XP_005269821                                                           15    9605
XP_006715890                                                           15    9605
XP_005250160                                                           15    9605
XP_005250159                                                           15    9605
XP_006712050                                                           15    9605
XP_005264311                                                           15    9605
XP_006712049                                                           15    9605
NP_001121687                                                           15    9605
>NP_444256
          Length = 1794

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VRKEVTNLI 9
           VRKEVTN+I
Sbjct: 271 VRKEVTNVI 279
>NP_444254
          Length = 1845

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VRKEVTNLI 9
           VRKEVTN+I
Sbjct: 271 VRKEVTNVI 279
>NP_444253
          Length = 1914

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VRKEVTNLI 9
           VRKEVTN+I
Sbjct: 271 VRKEVTNVI 279
>NP_444255
          Length = 1863

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VRKEVTNLI 9
           VRKEVTN+I
Sbjct: 271 VRKEVTNVI 279
>XP_005247551
          Length = 1855

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VRKEVTNLI 9
           VRKEVTN+I
Sbjct: 281 VRKEVTNVI 289
>XP_005247550
          Length = 1856

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VRKEVTNLI 9
           VRKEVTN+I
Sbjct: 281 VRKEVTNVI 289
>XP_006713712
          Length = 1923

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VRKEVTNLI 9
           VRKEVTN+I
Sbjct: 281 VRKEVTNVI 289
>XP_005247549
          Length = 1924

 Score = 19.6 bits (39), Expect =   509
 Identities = 8/9 (88%), Positives = 9/9 (100%)

Query: 1   VRKEVTNLI 9
           VRKEVTN+I
Sbjct: 281 VRKEVTNVI 289
>NP_055921
          Length = 1467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKEVTNLI 9
           RKEV NL+
Sbjct: 900 RKEVENLL 907
>XP_006713125
          Length = 1467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKEVTNLI 9
           RKEV NL+
Sbjct: 900 RKEVENLL 907
>XP_006713124
          Length = 1467

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RKEVTNLI 9
           RKEV NL+
Sbjct: 900 RKEVENLL 907
>NP_001242904
          Length = 873

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRKEVTN 7
           +R+EVTN
Sbjct: 310 IREEVTN 316
>NP_001008496
          Length = 882

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/7 (71%), Positives = 7/7 (100%)

Query: 1   VRKEVTN 7
           +R+EVTN
Sbjct: 310 IREEVTN 316
>NP_005742
          Length = 3907

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    KEVTNL 8
            KE+TNL
Sbjct: 2203 KEITNL 2208
>NP_671714
          Length = 3899

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    KEVTNL 8
            KE+TNL
Sbjct: 2195 KEITNL 2200
>NP_056477
          Length = 1749

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVTNL 8
           KE+TNL
Sbjct: 281 KEITNL 286
>NP_001268451
          Length = 793

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EVTNLI 9
           EVTNLI
Sbjct: 133 EVTNLI 138
>NP_001191055
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRKEVTNLI 9
           ++KE+TN +
Sbjct: 190 LKKEITNCL 198
>NP_001268449
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EVTNLI 9
           EVTNLI
Sbjct: 133 EVTNLI 138
>NP_006143
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRKEVTNLI 9
           ++KE+TN +
Sbjct: 190 LKKEITNCL 198
>NP_001191056
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRKEVTNLI 9
           ++KE+TN +
Sbjct: 190 LKKEITNCL 198
>NP_877954
          Length = 824

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EVTNLI 9
           EVTNLI
Sbjct: 133 EVTNLI 138
>NP_001268450
          Length = 880

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EVTNLI 9
           EVTNLI
Sbjct: 133 EVTNLI 138
>NP_115874
          Length = 840

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EVTNLI 9
           EVTNLI
Sbjct: 133 EVTNLI 138
>NP_003557
          Length = 1411

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    KEVTNL 8
            KE+TNL
Sbjct: 1156 KEITNL 1161
>XP_005260916
          Length = 581

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EVTNLI 9
           EVTNLI
Sbjct: 133 EVTNLI 138
>XP_006719694
          Length = 1465

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    KEVTNL 8
            KE+TNL
Sbjct: 1210 KEITNL 1215
>XP_005253431
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRKEVTNLI 9
           ++KE+TN +
Sbjct: 190 LKKEITNCL 198
>XP_006719140
          Length = 446

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRKEVTNLI 9
           ++KE+TN +
Sbjct: 137 LKKEITNCL 145
>XP_006719139
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRKEVTNLI 9
           ++KE+TN +
Sbjct: 190 LKKEITNCL 198
>XP_005253429
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRKEVTNLI 9
           ++KE+TN +
Sbjct: 190 LKKEITNCL 198
>XP_006719138
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRKEVTNLI 9
           ++KE+TN +
Sbjct: 190 LKKEITNCL 198
>XP_005253428
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRKEVTNLI 9
           ++KE+TN +
Sbjct: 190 LKKEITNCL 198
>XP_005253427
          Length = 499

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRKEVTNLI 9
           ++KE+TN +
Sbjct: 190 LKKEITNCL 198
>XP_005269821
          Length = 398

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EVTNLI 9
           EVTNLI
Sbjct: 346 EVTNLI 351
>XP_006715890
          Length = 3860

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    KEVTNL 8
            KE+TNL
Sbjct: 2156 KEITNL 2161
>XP_005250160
          Length = 3939

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    KEVTNL 8
            KE+TNL
Sbjct: 2235 KEITNL 2240
>XP_005250159
          Length = 3952

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3    KEVTNL 8
            KE+TNL
Sbjct: 2248 KEITNL 2253
>XP_006712050
          Length = 1716

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVTNL 8
           KE+TNL
Sbjct: 281 KEITNL 286
>XP_005264311
          Length = 1727

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVTNL 8
           KE+TNL
Sbjct: 281 KEITNL 286
>XP_006712049
          Length = 1728

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   KEVTNL 8
           KE+TNL
Sbjct: 260 KEITNL 265
>NP_001121687
          Length = 423

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/6 (100%), Positives = 6/6 (100%)

Query: 4   EVTNLI 9
           EVTNLI
Sbjct: 371 EVTNLI 376
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.320    0.136    0.332 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 446,871
Number of extensions: 742
Number of successful extensions: 42
Number of sequences better than 10000.0: 42
Number of HSP's gapped: 42
Number of HSP's successfully gapped: 42
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.8 bits)
S2: 28 (15.4 bits)

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