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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_000537                                                              18    1135
NP_001263689                                                           18    1135
NP_001119584                                                           18    1135
NP_001119586                                                           18    1135
NP_001263625                                                           18    1135
NP_001119590                                                           18    1135
NP_001263690                                                           18    1135
NP_001119585                                                           18    1135
NP_001263624                                                           18    1135
NP_001119588                                                           18    1135
NP_001119587                                                           18    1135
NP_001119589                                                           18    1135
NP_001008844                                                           16    5631
NP_004406                                                              16    5631
NP_004082                                                              16    7355
XP_005245806                                                           16    7355
XP_005245805                                                           16    7355
NP_055448                                                              16    7355
NP_742024                                                              15    9605
NP_689540                                                              15    9605
XP_006720086                                                           15    9605
XP_006720085                                                           15    9605
XP_006720084                                                           15    9605
XP_006713834                                                           15    9605
XP_006713833                                                           15    9605
XP_006713832                                                           15    9605
XP_006713831                                                           15    9605

>NP_000537
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           VRAMA YKQ
Sbjct: 157 VRAMAIYKQ 165



>NP_001263689
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           VRAMA YKQ
Sbjct: 118 VRAMAIYKQ 126



>NP_001119584
          Length = 393

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           VRAMA YKQ
Sbjct: 157 VRAMAIYKQ 165



>NP_001119586
          Length = 341

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           VRAMA YKQ
Sbjct: 157 VRAMAIYKQ 165



>NP_001263625
          Length = 302

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           VRAMA YKQ
Sbjct: 118 VRAMAIYKQ 126



>NP_001119590
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           VRAMA YKQ
Sbjct: 118 VRAMAIYKQ 126



>NP_001263690
          Length = 354

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           VRAMA YKQ
Sbjct: 118 VRAMAIYKQ 126



>NP_001119585
          Length = 346

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           VRAMA YKQ
Sbjct: 157 VRAMAIYKQ 165



>NP_001263624
          Length = 307

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           VRAMA YKQ
Sbjct: 118 VRAMAIYKQ 126



>NP_001119588
          Length = 209

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VRAMANYKQ 9
          VRAMA YKQ
Sbjct: 25 VRAMAIYKQ 33



>NP_001119587
          Length = 261

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VRAMANYKQ 9
          VRAMA YKQ
Sbjct: 25 VRAMAIYKQ 33



>NP_001119589
          Length = 214

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1  VRAMANYKQ 9
          VRAMA YKQ
Sbjct: 25 VRAMAIYKQ 33



>NP_001008844
          Length = 2272

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           +RA+ +YKQ
Sbjct: 463 LRALCDYKQ 471



>NP_004406
          Length = 2871

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/9 (55%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           +RA+ +YKQ
Sbjct: 463 LRALCDYKQ 471



>NP_004082
          Length = 437

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1   VRAMANYKQ 9
           +RA+ N+K+
Sbjct: 255 IRAVGNFKE 263



>XP_005245806
          Length = 241

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  VRAMANYKQ 9
          +RA+ N+K+
Sbjct: 59 IRAVGNFKE 67



>XP_005245805
          Length = 249

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1  VRAMANYKQ 9
          +RA+ N+K+
Sbjct: 67 IRAVGNFKE 75



>NP_055448
          Length = 1173

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   RAMANYKQ 9
           RA+A YKQ
Sbjct: 631 RALAIYKQ 638



>NP_742024
          Length = 478

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RAMANYKQ 9
          RA+ +YKQ
Sbjct: 83 RALRDYKQ 90



>NP_689540
          Length = 723

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VRAMANYK 8
           +RA++NY+
Sbjct: 554 LRALSNYR 561



>XP_006720086
          Length = 703

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VRAMANYK 8
           +RA++NY+
Sbjct: 527 LRALSNYR 534



>XP_006720085
          Length = 704

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VRAMANYK 8
           +RA++NY+
Sbjct: 528 LRALSNYR 535



>XP_006720084
          Length = 730

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/8 (50%), Positives = 8/8 (100%)

Query: 1   VRAMANYK 8
           +RA++NY+
Sbjct: 554 LRALSNYR 561



>XP_006713834
          Length = 458

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RAMANYKQ 9
          RA+ +YKQ
Sbjct: 63 RALRDYKQ 70



>XP_006713833
          Length = 470

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RAMANYKQ 9
           RA+ +YKQ
Sbjct: 114 RALRDYKQ 121



>XP_006713832
          Length = 480

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2  RAMANYKQ 9
          RA+ +YKQ
Sbjct: 85 RALRDYKQ 92



>XP_006713831
          Length = 509

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   RAMANYKQ 9
           RA+ +YKQ
Sbjct: 114 RALRDYKQ 121



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.322    0.121    0.320 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 355,411
Number of extensions: 263
Number of successful extensions: 27
Number of sequences better than 10000.0: 27
Number of HSP's gapped: 27
Number of HSP's successfully gapped: 27
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.1 bits)
S2: 28 (15.4 bits)