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Blast-Search of mutated query peptide against human proteome

BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_001275701                                                           22     134
NP_004561                                                              22     134
NP_001275703                                                           22     134
XP_005253450                                                           22     134
XP_006719151                                                           22     134
NP_005392                                                              18    1935
XP_005271739                                                           17    3301
XP_005272029                                                           17    3301
NP_003469                                                              17    3301
NP_055221                                                              17    4312
NP_659482                                                              17    4312
XP_005258568                                                           17    4312
NP_789781                                                              17    4312
NP_004516                                                              16    5631
XP_006712590                                                           16    5631
XP_006712589                                                           16    5631
NP_005566                                                              16    7355
NP_787116                                                              16    7355
NP_997191                                                              16    7355
NP_001152956                                                           16    7355
XP_006711682                                                           16    7355
NP_076985                                                              15    9605
NP_001123990                                                           15    9605
NP_060841                                                              15    9605
XP_006716429                                                           15    9605
XP_006716427                                                           15    9605
XP_006716426                                                           15    9605
XP_006716425                                                           15    9605
XP_006716424                                                           15    9605
XP_006716423                                                           15    9605
XP_006716422                                                           15    9605
XP_006716421                                                           15    9605

>NP_001275701
          Length = 1486

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VQAVMFEWN 9
           VQAV FEWN
Sbjct: 802 VQAVKFEWN 810



>NP_004561
          Length = 1445

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VQAVMFEWN 9
           VQAV FEWN
Sbjct: 761 VQAVKFEWN 769



>NP_001275703
          Length = 1264

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VQAVMFEWN 9
           VQAV FEWN
Sbjct: 580 VQAVKFEWN 588



>XP_005253450
          Length = 1486

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VQAVMFEWN 9
           VQAV FEWN
Sbjct: 802 VQAVKFEWN 810



>XP_006719151
          Length = 1486

 Score = 21.6 bits (44), Expect =   134
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VQAVMFEWN 9
           VQAV FEWN
Sbjct: 802 VQAVKFEWN 810



>NP_005392
          Length = 1187

 Score = 17.7 bits (34), Expect =  1935
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 2   QAVMFEWN 9
           QAV++ WN
Sbjct: 248 QAVIYRWN 255



>XP_005271739
          Length = 721

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   AVMFEWN 9
           AV+F WN
Sbjct: 542 AVLFAWN 548



>XP_005272029
          Length = 213

 Score = 16.9 bits (32), Expect =  3301
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 1   VQAVMFEW 8
           VQAV F W
Sbjct: 184 VQAVSFTW 191



>NP_003469
          Length = 780

 Score = 16.9 bits (32), Expect =  3301
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 3   AVMFEWN 9
           AV+F WN
Sbjct: 601 AVLFAWN 607



>NP_055221
          Length = 557

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/6 (83%), Positives = 6/6 (100%)

Query: 3   AVMFEW 8
           AV+FEW
Sbjct: 360 AVVFEW 365



>NP_659482
          Length = 595

 Score = 16.5 bits (31), Expect =  4312
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VQAVMFEW 8
           VQAV+ EW
Sbjct: 128 VQAVIREW 135



>XP_005258568
          Length = 1029

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   VMFEW 8
           VMFEW
Sbjct: 223 VMFEW 227



>NP_789781
          Length = 1048

 Score = 16.5 bits (31), Expect =  4312
 Identities = 5/5 (100%), Positives = 5/5 (100%)

Query: 4   VMFEW 8
           VMFEW
Sbjct: 223 VMFEW 227



>NP_004516
          Length = 4655

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    VQAVMFEWN 9
            +QAV ++W+
Sbjct: 4084 IQAVDYDWD 4092



>XP_006712590
          Length = 3897

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    VQAVMFEWN 9
            +QAV ++W+
Sbjct: 3326 IQAVDYDWD 3334



>XP_006712589
          Length = 4660

 Score = 16.2 bits (30), Expect =  5631
 Identities = 4/9 (44%), Positives = 8/9 (88%)

Query: 1    VQAVMFEWN 9
            +QAV ++W+
Sbjct: 4089 IQAVDYDWD 4097



>NP_005566
          Length = 1025

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   VQAVMFEWN 9
           VQA+   WN
Sbjct: 198 VQALQVTWN 206



>NP_787116
          Length = 1011

 Score = 15.8 bits (29), Expect =  7355
 Identities = 5/9 (55%), Positives = 6/9 (66%)

Query: 1   VQAVMFEWN 9
           VQA+   WN
Sbjct: 184 VQALQVTWN 192



>NP_997191
          Length = 1888

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   VMFEWN 9
           +MFEW+
Sbjct: 167 MMFEWS 172



>NP_001152956
          Length = 1736

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4   VMFEWN 9
           +MFEW+
Sbjct: 167 MMFEWS 172



>XP_006711682
          Length = 1739

 Score = 15.8 bits (29), Expect =  7355
 Identities = 4/6 (66%), Positives = 6/6 (100%)

Query: 4  VMFEWN 9
          +MFEW+
Sbjct: 18 MMFEWS 23



>NP_076985
          Length = 1104

 Score = 15.4 bits (28), Expect =  9605
 Identities = 4/6 (66%), Positives = 5/6 (83%)

Query: 4   VMFEWN 9
           V+F WN
Sbjct: 736 VVFSWN 741



>NP_001123990
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QAVMFEW 8
          QAV+ EW
Sbjct: 58 QAVLQEW 64



>NP_060841
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QAVMFEW 8
          QAV+ EW
Sbjct: 58 QAVLQEW 64



>XP_006716429
          Length = 297

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QAVMFEW 8
          QAV+ EW
Sbjct: 58 QAVLQEW 64



>XP_006716427
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QAVMFEW 8
          QAV+ EW
Sbjct: 58 QAVLQEW 64



>XP_006716426
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QAVMFEW 8
          QAV+ EW
Sbjct: 58 QAVLQEW 64



>XP_006716425
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QAVMFEW 8
          QAV+ EW
Sbjct: 58 QAVLQEW 64



>XP_006716424
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QAVMFEW 8
          QAV+ EW
Sbjct: 58 QAVLQEW 64



>XP_006716423
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QAVMFEW 8
          QAV+ EW
Sbjct: 58 QAVLQEW 64



>XP_006716422
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QAVMFEW 8
          QAV+ EW
Sbjct: 58 QAVLQEW 64



>XP_006716421
          Length = 532

 Score = 15.4 bits (28), Expect =  9605
 Identities = 5/7 (71%), Positives = 6/7 (85%)

Query: 2  QAVMFEW 8
          QAV+ EW
Sbjct: 58 QAVLQEW 64



  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.329    0.128    0.472 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 435,082
Number of extensions: 213
Number of successful extensions: 32
Number of sequences better than 10000.0: 32
Number of HSP's gapped: 32
Number of HSP's successfully gapped: 32
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (16.3 bits)
S2: 28 (15.4 bits)