Cancertope

In silico Platform for designing genome-based
Personalized immunotherapy or Vaccine against Cancer

Blast-Search of mutated query peptide against human proteome

BLAST Search Results
BLASTP 2.2.15 [Oct-15-2006]


Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= User_input
         (9 letters)

Database: HUMAN_PROTEOME_FASTA 
           71,491 sequences; 45,948,801 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

NP_444256                                                              18    1135
NP_444254                                                              18    1135
NP_444253                                                              18    1135
NP_444255                                                              18    1135
XP_005247551                                                           18    1135
XP_005247550                                                           18    1135
XP_006713712                                                           18    1135
XP_005247549                                                           18    1135
NP_004225                                                              16    5631
NP_001020279                                                           16    5631
NP_002276                                                              16    5631
XP_005259469                                                           16    5631
XP_005264000                                                           16    5631
XP_006712586                                                           16    5631
XP_005263999                                                           16    5631
NP_001273661                                                           16    7355
NP_078995                                                              16    7355
NP_001273662                                                           16    7355
XP_006720043                                                           16    7355
NP_079223                                                              15    9605
XP_005269164                                                           15    9605
XP_006713820                                                           15    9605
XP_005247850                                                           15    9605
XP_005247848                                                           15    9605
XP_006713819                                                           15    9605
XP_005247847                                                           15    9605
XP_006713818                                                           15    9605
XP_006713817                                                           15    9605
XP_006713816                                                           15    9605
XP_005247846                                                           15    9605
>NP_444256
          Length = 1794

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VPLQQSARV 9
           VPLQ SARV
Sbjct: 199 VPLQPSARV 207
>NP_444254
          Length = 1845

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VPLQQSARV 9
           VPLQ SARV
Sbjct: 199 VPLQPSARV 207
>NP_444253
          Length = 1914

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VPLQQSARV 9
           VPLQ SARV
Sbjct: 199 VPLQPSARV 207
>NP_444255
          Length = 1863

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VPLQQSARV 9
           VPLQ SARV
Sbjct: 199 VPLQPSARV 207
>XP_005247551
          Length = 1855

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VPLQQSARV 9
           VPLQ SARV
Sbjct: 209 VPLQPSARV 217
>XP_005247550
          Length = 1856

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VPLQQSARV 9
           VPLQ SARV
Sbjct: 209 VPLQPSARV 217
>XP_006713712
          Length = 1923

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VPLQQSARV 9
           VPLQ SARV
Sbjct: 209 VPLQPSARV 217
>XP_005247549
          Length = 1924

 Score = 18.5 bits (36), Expect =  1135
 Identities = 8/9 (88%), Positives = 8/9 (88%)

Query: 1   VPLQQSARV 9
           VPLQ SARV
Sbjct: 209 VPLQPSARV 217
>NP_004225
          Length = 738

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VPLQQSAR 8
           +P+QQS R
Sbjct: 306 IPVQQSVR 313
>NP_001020279
          Length = 1251

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VPLQQSAR 8
           VP+QQS R
Sbjct: 161 VPVQQSKR 168
>NP_002276
          Length = 1226

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VPLQQSAR 8
           VP+QQS R
Sbjct: 136 VPVQQSKR 143
>XP_005259469
          Length = 734

 Score = 16.2 bits (30), Expect =  5631
 Identities = 5/8 (62%), Positives = 7/8 (87%)

Query: 1   VPLQQSAR 8
           +P+QQS R
Sbjct: 302 IPVQQSVR 309
>XP_005264000
          Length = 1226

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VPLQQSAR 8
           VP+QQS R
Sbjct: 136 VPVQQSKR 143
>XP_006712586
          Length = 1262

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VPLQQSAR 8
           VP+QQS R
Sbjct: 188 VPVQQSKR 195
>XP_005263999
          Length = 1278

 Score = 16.2 bits (30), Expect =  5631
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 1   VPLQQSAR 8
           VP+QQS R
Sbjct: 188 VPVQQSKR 195
>NP_001273661
          Length = 344

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VPLQQSARV 9
          VPL+  ARV
Sbjct: 69 VPLEHRARV 77
>NP_078995
          Length = 336

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VPLQQSARV 9
          VPL+  ARV
Sbjct: 69 VPLEHRARV 77
>NP_001273662
          Length = 236

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VPLQQSARV 9
          VPL+  ARV
Sbjct: 69 VPLEHRARV 77
>XP_006720043
          Length = 190

 Score = 15.8 bits (29), Expect =  7355
 Identities = 6/9 (66%), Positives = 7/9 (77%)

Query: 1  VPLQQSARV 9
          VPL+  ARV
Sbjct: 69 VPLEHRARV 77
>NP_079223
          Length = 995

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLQQSARV 9
           P+QQSA V
Sbjct: 480 PVQQSALV 487
>XP_005269164
          Length = 598

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 6/8 (75%)

Query: 2  PLQQSARV 9
          PL Q ARV
Sbjct: 8  PLHQRARV 15
>XP_006713820
          Length = 597

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLQQSARV 9
           P+QQSA V
Sbjct: 480 PVQQSALV 487
>XP_005247850
          Length = 614

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLQQSARV 9
           P+QQSA V
Sbjct: 480 PVQQSALV 487
>XP_005247848
          Length = 897

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLQQSARV 9
           P+QQSA V
Sbjct: 480 PVQQSALV 487
>XP_006713819
          Length = 925

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLQQSARV 9
           P+QQSA V
Sbjct: 394 PVQQSALV 401
>XP_005247847
          Length = 983

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLQQSARV 9
           P+QQSA V
Sbjct: 468 PVQQSALV 475
>XP_006713818
          Length = 999

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLQQSARV 9
           P+QQSA V
Sbjct: 468 PVQQSALV 475
>XP_006713817
          Length = 999

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLQQSARV 9
           P+QQSA V
Sbjct: 468 PVQQSALV 475
>XP_006713816
          Length = 999

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLQQSARV 9
           P+QQSA V
Sbjct: 468 PVQQSALV 475
>XP_005247846
          Length = 1011

 Score = 15.4 bits (28), Expect =  9605
 Identities = 6/8 (75%), Positives = 7/8 (87%)

Query: 2   PLQQSARV 9
           P+QQSA V
Sbjct: 480 PVQQSALV 487
  Database: HUMAN_PROTEOME_FASTA
    Posted date:  May 26, 2014 10:46 AM
  Number of letters in database: 45,948,801
  Number of sequences in database:  71,491
  
Lambda     K      H
   0.318    0.125    0.325 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 71491
Number of Hits to DB: 445,439
Number of extensions: 421
Number of successful extensions: 30
Number of sequences better than 10000.0: 30
Number of HSP's gapped: 30
Number of HSP's successfully gapped: 30
Length of query: 9
Length of database: 45,948,801
Length adjustment: 0
Effective length of query: 9
Effective length of database: 45,948,801
Effective search space: 413539209
Effective search space used: 413539209
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 28 (15.9 bits)
S2: 28 (15.4 bits)

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